Quiz 2 of 4 covers Molecular Techniques. Topics include separation and detection, nucleic acid amplification, DNA sequence analysis and other techniques. Refer to Part II in ASCP Exam Content Outline for a list of subtopics.

5 Sample Questions

Which statement(s) is true about protein chain reaction (PCR)?

  • A. PCR produces identical DNA strands.
  • B. PCR requires forward and reverse primers to amplify target sequence.
  • C. PCR requires thermocyclers to stabilize the temperature at the denaturing, annealing and extension steps.
  • D. PCR components include DNA polymerase and DNA ligase.

The mixture for Sanger sequencing contains single-stranded DNA, DNA polymerase, deoxyribonucleotides, primer and dideoxyribonucleotide. Which component in the mixture when inserted terminates replication?

  • A. DNA polymerase
  • B. deoxyribonucleotides
  • C. primer
  • D. dideoxyribonucleotide

Several blotting methods are used to transfer DNA to nitrocellulose prior to hybridization with probes. Why is the capillary transfer method preferred over electrophoretic and vacuum transfer methods?

  • A. relatively inexpensive to use
  • B. low maintenance cost on required equipment
  • C. rapid reaction (short run time)
  • D. uniform staining

Which condition of the membrane gives low signal interference ("background noise") when analyzing an electrophoresis result?

  • A. positive charge
  • B. neutral charge
  • C. dry spots
  • D. exposure to UV light

Large fragments of resolved DNA must undergo which processes before blotting? (Select all that apply.)

  • A. Depurination
  • B. Denaturation
  • C. Depyrimidation
  • D. Dehydration

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