Transcription Sample Questions

10 Questions

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Protein Quizzes & Trivia

Questions and Answers
  • 1. 
    • A. 

      500 bp

    • B. 

      400 bp

    • C. 

      300 bp

    • D. 

      100 bp

    • E. 

      70 bp

  • 2. 
    The hypothetical “stimulin” gene with two exons encoding a protein of 100 amino acids is found to have abnormal expression in cell culture.  Which of the following mutations would produce a 500-bp stimulin mRNA and a 133-amino acid peptide that reacts with antibodies to stimulin protein?
    • A. 

      Splice junction mutation preventing RNA splicing

    • B. 

      Frame-shift mutation is codon #2

    • C. 

      Silent point mutation in the third nucleotide of codon #50

    • D. 

      Nonsense mutation at codon #2

    • E. 

      Deletion of exon 1

  • 3. 
    In contrast to DNA polymerase, RNA polymerase
    • A. 

      Fills in the gap between Okazaki fragments

    • B. 

      Works only in a 5’ to 3’ direction

    • C. 

      Edits as it synthesizes

    • D. 

      Synthesizes RNA primer to initiate DNA synthesis

    • E. 

      Adds nucleoside monophosphates to the growing polynucleotides

  • 4. 
    The removal of introns and subsequent self-splicing of adjacent exons occurs in some portions of primary ribosomal RNA transcripts.  The splicing of introns in messenger RNA precursors is
    • A. 

      RNA-catalyzed in the absence of protein

    • B. 

      Self-splicing

    • C. 

      Carried out by spliceosomes

    • D. 

      Controlled by RNA polymerase

    • E. 

      Regulated by RNA helicase

  • 5. 
    A promoter site on DNA
    • A. 

      Transcribes repressor

    • B. 

      Initiates transcription

    • C. 

      Codes for RNA polymerase

    • D. 

      Regulates termination

    • E. 

      Translates specific proteins

  • 6. 
    The  o factor found in many bacteria is best described as a
    • A. 

      Subunit of RNA polymerase responsible for the specificity of the initiation of transcription of RNA to DNA

    • B. 

      Subunit of DNA polymerase that allows for synthesis in both 5’ to 3’and 3’ to 5’ directions

    • C. 

      Subunit of the 50S ribosome that catalyzes peptide bond synthesis

    • D. 

      Subunit of the 30S ribosome to which mRNA binds

    • E. 

      Factor that forms the bridge between 30S and 50S particles constituting the 70S ribosome

  • 7. 
    An immigrant from Eastern Europe is rushed into the emergency room with nausea, vomiting, diarrhea, and abdominal pain.  His family indicates he has eaten wild mushrooms.  They have brought a bag of fresh, uncooked mushrooms from a batch he had not yet prepared.  You note the presence of Amanita phalloides, the death-cap mushroom.  Care is supportive.  A major toxin of the death-cap mushroom is the hepatotoxic octapeptide a-amanitin, which inhibits
    • A. 

      DNA primase

    • B. 

      RNA nuclease

    • C. 

      DNA ligase

    • D. 

      RNA polymerase

    • E. 

      RNA/DNA endonuclease

  • 8. 
    The consensus sequence 5’TATAAAA 3’ found in eukaryotic genes is quite similar to a consensus sequence observed in prokaryotes.  It is important as the
    • A. 

      Only site of binding of RNA polymerase III

    • B. 

      Promoter for all RNA polymerases

    • C. 

      Termination site for RNA polymerase II

    • D. 

      Major binding site of RNA polymerase I

    • E. 

      First site of binding of a transcription factor for RNA polymerase II

  • 9. 
    The so-called caps of RNA molecules
    • A. 

      Allow tRNA to be processed

    • B. 

      Occur at the 3’ end of tRNA

    • C. 

      Are composed of poly A

    • D. 

      Are unique to eukaryotic mRNA

    • E. 

      Allow correct translation of prokaryotic mRNA

  • 10. 
    In bacterial RNA synthesis, the function of factor p is to
    • A. 

      Bind catabolie repressor to the promoter region

    • B. 

      Increase the rate of RNA synthesis

    • C. 

      Eliminate the binding of RNA polymerase to the promoter

    • D. 

      Participate in the proper termination of transcription

    • E. 

      Allow proper initiation of transcription