RNA Metabolism II

27 Questions

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RNA Quizzes & Trivia

Questions and Answers
  • 1. 
    Of the following eukaryotic RNA polymerases which is the only one not located in the nucleoplasm?
    • A. 

      Type I

    • B. 

      Type II

    • C. 

      Type III

  • 2. 
    Which type of RNA polymerase produces tRNA and 5S rRNA?
    • A. 

      Type I

    • B. 

      Type II

    • C. 

      Type III

  • 3. 
    Suppose while looking for magical mushrooms to go on a magic carpet ride, an amateur mycologist ingested a Death Cap mushroom which contains a-amanitin, which RNA polymerase would be STRONGLY inhibited?
    • A. 

      Type I

    • B. 

      Type II

    • C. 

      Type III

  • 4. 
    In eukaryotes, promoter regions like the _______ box recruit transcription factors to bind in order to initiate transcription.
  • 5. 
    Which two crucial enzymatic activities does TFIIH carry out?
    • A. 

      Kinase

    • B. 

      Phosphorylase

    • C. 

      Helicase

    • D. 

      Endonuclease

    • E. 

      Isomerase

  • 6. 
    RNA polymerase can bind to the DNA and start transcription without general transcription factors such as TBP, TFIIB, and TFIIH
    • A. 

      True

    • B. 

      False

  • 7. 
    After synthesis of 60-70 nucleotides, which two transcription factors are released from the complex?
    • A. 

      TFIIE

    • B. 

      TFIID

    • C. 

      TFIIJ

    • D. 

      TFIIB

    • E. 

      TFIIH

  • 8. 
    How is RNA Pol II recycled?
    • A. 

      Hydrolysis

    • B. 

      Phosphorylation

    • C. 

      Dephosphorylation

    • D. 

      Dehydration

  • 9. 
    The carboxy-terminal domain (CTD) of RNA polymerase II (RNAPII) which undergoes extensive posttranslational modification, and is essential for transcriptional initiation and elongation is comprised of 52 repeats of...
    • A. 

      SPSTPSY

    • B. 

      TSPRSPS

    • C. 

      YSPTSPS

    • D. 

      AAUUAAA

  • 10. 
    _______ is a form of RNA end modification that is carried out by the nuclear enzyme __________ that protects the RNA from degradation and is needed for initiation of translation
    • A. 

      Splicing;guanylyltransferase

    • B. 

      Capping; methyltransferase

    • C. 

      Poly-A tail addition; methyltransferase

    • D. 

      Capping; guanylyltransferase

  • 11. 
    What is the polyadenylation signal sequence used in the addition of a poly-A tail which stabilizes the mRNA and facilitates the nuclear export of mRNA 
    • A. 

      YSPTSPS

    • B. 

      AAUAAA

    • C. 

      UAGAAA

    • D. 

      TATAAA

  • 12. 
    Removal of ________ splices the flanking _______ together.
    • A. 

      Introns; exons

    • B. 

      Exons; introns

  • 13. 
    The spliceosome is the machinery to carry out splicing and consists of ______ transesterfications.
    • A. 

      1

    • B. 

      2

    • C. 

      3

    • D. 

      4

  • 14. 
    All eukaryotic introns start with ____ and end with _____.
    • A. 

      GU; AG

    • B. 

      AG; GU

    • C. 

      UA; AG

    • D. 

      CA; UA

  • 15. 
    At the 5' splice site AGGUAAGU, which pair of bases are part of the upstream exon?
    • A. 

      GU

    • B. 

      AA

    • C. 

      AG

    • D. 

      UA

  • 16. 
    In lariat formation, the ____ of a specific adenosine acts as a nucleophile and attacks the _____  splice site to form a lariat.
    • A. 

      3'OH; 5'

    • B. 

      2'OH; 3'

    • C. 

      2'OH; 5'

    • D. 

      5'OH; 3'

  • 17. 
    The 3'OH of the 5' exon acts as a nucleophile to complete the splicing reaction.
    • A. 

      True

    • B. 

      False

  • 18. 
    SnRNPs complex with other proteins to form the ______________.
  • 19. 
    A preassembled complex of which snRNPs form to complete the spliceosome?
    • A. 

      U1

    • B. 

      U2

    • C. 

      U3

    • D. 

      U4

    • E. 

      U5

    • F. 

      U6

    • G. 

      U7

  • 20. 
    A typical fully processed mRNA would not possess which on the following?
    • A. 

      5' Cap

    • B. 

      Poly (A) tail

    • C. 

      Coding region

    • D. 

      Exons

    • E. 

      Introns

  • 21. 
    The method of degradation of mRNA in bacteria is due to....
    • A. 

      Cuts in mRNA by an endoribonuclease followed by 5' to 3' exoribonuclease action

    • B. 

      Poly A shortening that acts as a timer

    • C. 

      Cuts in mRNA by an endoribonuclease followed by 3' to 5' exoribonuclease action

    • D. 

      Decapping followed by 5' to 3' degradation

  • 22. 
    In the processing of pre-RNA transcripts in eukaryotes, chemical modifications are mostly methylations and isomerations of uridine to _____________.
  • 23. 
    The antibiotic that inhibits bacterial protein synthesis by binding to the 23S rRNA inhibiting translocation is called?
    • A. 

      Rifampin

    • B. 

      Actinomycin

    • C. 

      Ciprofloxacin

    • D. 

      Erythromycin

  • 24. 
    Resistance to the above mentioned antibiotic in question #23 (The antibiotic that inhibits bacterial protein synthesis by binding to the 23S rRNA) is mediated by a plasmid borne...
    • A. 

      RNA kinase

    • B. 

      DNA methylase

    • C. 

      RNA methylase

    • D. 

      Protein synthetase

  • 25. 
    Bacterial and eukaryotic tRNA are similar in that they both have a CCA sequence at the 3' end, but in eukaryotes this is added
    • A. 

      Pretranscriptionally

    • B. 

      Posttranscriptionally

  • 26. 
    Which one of these is not one of the 3 enzymatic activities of reverse transcriptase?
    • A. 

      DNA degradation

    • B. 

      RNA dependent DNA synthesis (RNase H like activity)

    • C. 

      RNA degradation

    • D. 

      DNA-dependent DNA synthesis

  • 27. 
    __________ is a special reverse transcriptase that consists of protein and an RNA strand which it uses as a template to elongate telomeres that are shortened after each replication cycle.