RNA Metabolism I

15 Questions

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RNA Quizzes & Trivia

Questions and Answers
  • 1. 
    Which of the following does not occur in the cytoplasm
    • A. 

      Prokaryotic transcription

    • B. 

      Eukaryotic transcription

    • C. 

      Prokaryotic translation

    • D. 

      Eukaryotic translation

  • 2. 
    The ___________ strand is written the same as the RNA strand except for T's being read as U's
  • 3. 
    The binding of what creates a holoenzyme in bacterial transcription that confers specificity for the promoter site
    • A. 

      Pi factor

    • B. 

      Rho factor

    • C. 

      Sigma factor

    • D. 

      Tau factor

  • 4. 
    The region to which RNA polymerase can bind in order to initiate transcription extends from ____ base pairs before the start site and ____ base pairs after the transcription start site
    • A. 

      8; 9

    • B. 

      30; 70

    • C. 

      10; 35

    • D. 

      70; 30

  • 5. 
    What does RNA polymerase use to carry out proofreading?
    • A. 

      ATP

    • B. 

      GTP

    • C. 

      A primer

    • D. 

      Unable to proofread

  • 6. 
    RNA polymerase __________ supercoils DNA to make it easier for transcription
  • 7. 
    Please identify the four consensus sequences mentioned in class
    • A. 

      UP element

    • B. 

      - 35 region

    • C. 

      +10 region

    • D. 

      Pribnow box

    • E. 

      A at -1

    • F. 

      A at +1

  • 8. 
    Which is not one of the factors that increases promoter strength
    • A. 

      The consensus sequences

    • B. 

      The distance of the sequences from the transcription start site

    • C. 

      The spacing between the consensus sequences

    • D. 

      Positive supercoiling by topoisomerases

    • E. 

      Negative supercoiling by topoisomerases

  • 9. 
    Going from a closed complex to an open complex in step two of transcription does not require ATP consumption
    • A. 

      True

    • B. 

      False

  • 10. 
    In the fourth step of transcription, the sigma factor is released after the first ____ nucleotides.
    • A. 

      8-9

    • B. 

      15-17

    • C. 

      7

    • D. 

      35

  • 11. 
    The Rho factor is an ATP ______ helicase that binds to a _____ rich region in the nascent RNA chain upstream of the termination site and eventually unwinds the RNA/DNA hybrid.
    • A. 

      Independent; GC

    • B. 

      Dependent; UA

    • C. 

      Dependent; CA

    • D. 

      Independent; CA

  • 12. 
    The Rho factor independent mechanism causes termination by the combined action of the _____ UA bonding in the hybrid and the _______ GC bonding in the newly formed RNA
    • A. 

      Strong; weak

    • B. 

      Weak; weak

    • C. 

      Strong; strong

    • D. 

      Weak; strong

  • 13. 
    This antibiotic works by binding to RNA polymerase and changing it's conformation so it cannot initiate RNA synthesis
    • A. 

      Actinomycin

    • B. 

      Alpha-aminitin

    • C. 

      Rifampin

    • D. 

      Acridine

  • 14. 
    Actinomycin's mechanism of action is that it intercalates between two successive ______ basepairs to prevent the double stranded DNA from being an effective template for RNA synthesis
    • A. 

      AT

    • B. 

      AU

    • C. 

      GC

  • 15. 
    _______ basepairs remain unwound during transcription and the RNA/DNA hybrid stays limited to about _____ basepairs
    • A. 

      8-9;7

    • B. 

      15-17; 7

    • C. 

      7;15-17

    • D. 

      30; 70