Restriction Enzyme Assessment
This will test your knowledge of restriction enzymes and gel electrophoresis.
- 1.
Which option BEST describes sticky ends.
- A.
Sticky ends are DNA fragments that carry a higher charge than normal after they have been cleaved by restriction enzymes.
- B.
Sticky ends are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other.
- C.
Sticky ends are DNA fragments cleaved by a restriction enzyme so that both strands are the same length.
- D.
Sticky ends are DNA fragments that attract a carbohydrate molecule to one end after being cleaved by a restriction enzyme.
Correct Answer
B. Sticky ends are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other. -
- 2.
Restriction enzymes cleave a ___________________ specific sequence of bases.
Correct Answer
specific
certain - 3.
In nature, the purpose of restriction enzymes is to
- A.
Protect the bacterium from virus attack by not allowing the virus to attach to the cell wall.
- B.
Protect the bacterium from the DNA of other organisms the bacterium infects.
- C.
Protect the bacterium from replicating its DNA at the wrong time.
- D.
Protect the bacterium from virus attack by cutting up foreign DNA.
Correct Answer
D. Protect the bacterium from virus attack by cutting up foreign DNA. -
- 4.
Restriction enzymes
- A.
Bind together strands of DNA.
- B.
Bind RNA fragments together.
- C.
Cut DNA at specific sites.
- D.
Stop transcription.
Correct Answer
C. Cut DNA at specific sites. -
- 5.
Gel electrophoresis sorts DNA molecules on the basis of their
- A.
Ability to bind to mRNA.
- B.
Size.
- C.
Solubility in water.
- D.
Solubility in the gel.
Correct Answer
B. Size. -
- 6.
Cutting DNA with a particular restriction enzyme produces ___________ ____________ that can be separated by gel electrophoresis.
Correct Answer
restriction fragments
restriction fragment - 7.
DNA ligase binds
- A.
Introns together.
- B.
Exons together.
- C.
Polymerase to the promoter.
- D.
Nucleotides together.
Correct Answer
D. Nucleotides together. -
- 8.
Based upon the picture provided, which of these are most likely to be true.
- A.
The defendant may be not guilty. The recovered blood has too many bands.
- B.
The defendant may be not guilty. The defendant's blood has one band not seen in the blood sample from the clothes.
- C.
The defendant may be guilty. The bands from the blood on the clothes matches with the ones from the victim's blood.
- D.
The defendant is may be guilty. The defendant's blood has one band not seen in the blood sample from the clothes.
Correct Answer
C. The defendant may be guilty. The bands from the blood on the clothes matches with the ones from the victim's blood. -
- 9.
Why is a gel electrophoresis often followed by a Southern blot?
- A.
A Southern blot is required to properly align the bands for analysis by technicians.
- B.
The gel is difficult to handle; the Southern blot allows for analysis by technicians.
- C.
The gel electrophoresis technique is messy; the Southern blot allows technicians to see the sample better.
- D.
The gel evaporates quickly during electrophoresis; the Southern blot slows down evaporation.
Correct Answer
B. The gel is difficult to handle; the Southern blot allows for analysis by technicians. -
- 10.
BamHI, HindIII, and EcoRI are all examples of
- A.
Genes.
- B.
DNA ligands.
- C.
Restriction enzymes.
- D.
Southern blots.
Correct Answer
C. Restriction enzymes. -
- 11.
The gel in a gel electrophoresis is
- A.
Sucrolose.
- B.
Pectrolose.
- C.
Pentarose.
- D.
Agarose.
Correct Answer
D. Agarose. -
- 12.
A vector is
- A.
Used to carry foreign genes or DNA fragments.
- B.
Divided by the charge of the DNA.
- C.
Carries restriction enzymes to their restriction sites.
- D.
Only used in matching DNA samples.
Correct Answer
A. Used to carry foreign genes or DNA fragments. -
- 13.
________________ ends are required to create a recombinant DNA molecule.
Correct Answer
Sticky - 14.
All restriction enzyme recognition sites
- A.
Are never read the same forward and backward.
- B.
End with CGT.
- C.
Begin with CGT.
- D.
Are read the same forward and backward.
Correct Answer
D. Are read the same forward and backward. -
- 15.
____________________ is a diagram of DNA showing the cut sites of a series of restriction enzymes. (The answer may be more than one word.)
Correct Answer
A restriction map
Restriction map
Restriction maps
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Current Version
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Mar 21, 2022Quiz Edited by
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May 16, 2010Quiz Created by
Jcorban
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