Restriction Enzyme Assessment
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This will test your knowledge of restriction enzymes and gel electrophoresis.
- 1.Which option BEST describes sticky ends.
- A.
Sticky ends are DNA fragments that carry a higher charge than normal after they have been cleaved by restriction enzymes.
- B.
Sticky ends are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other.
- C.
Sticky ends are DNA fragments cleaved by a restriction enzyme so that both strands are the same length.
- D.
Sticky ends are DNA fragments that attract a carbohydrate molecule to one end after being cleaved by a restriction enzyme.
- 2.
- 3.
- A.
Protect the bacterium from virus attack by not allowing the virus to attach to the cell wall.
- B.
Protect the bacterium from the DNA of other organisms the bacterium infects.
- C.
Protect the bacterium from replicating its DNA at the wrong time.
- D.
Protect the bacterium from virus attack by cutting up foreign DNA.
- 4.Restriction enzymes
- A.
Bind together strands of DNA.
- B.
Bind RNA fragments together.
- C.
Cut DNA at specific sites.
- D.
Stop transcription.
- 5.Gel electrophoresis sorts DNA molecules on the basis of their
- A.
Ability to bind to mRNA.
- B.
Size.
- C.
Solubility in water.
- D.
Solubility in the gel.
- 6.Cutting DNA with a particular restriction enzyme produces ___________ ____________ that can be separated by gel electrophoresis.
- 7.DNA ligase binds
- A.
Introns together.
- B.
Exons together.
- C.
Polymerase to the promoter.
- D.
Nucleotides together.
- 8.
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Based upon the picture provided, which of these are most likely to be true.- A.
The defendant may be not guilty. The recovered blood has too many bands.
- B.
The defendant may be not guilty. The defendant's blood has one band not seen in the blood sample from the clothes.
- C.
The defendant may be guilty. The bands from the blood on the clothes matches with the ones from the victim's blood.
- D.
The defendant is may be guilty. The defendant's blood has one band not seen in the blood sample from the clothes.
- 9.
- A.
A Southern blot is required to properly align the bands for analysis by technicians.
- B.
The gel is difficult to handle; the Southern blot allows for analysis by technicians.
- C.
The gel electrophoresis technique is messy; the Southern blot allows technicians to see the sample better.
- D.
The gel evaporates quickly during electrophoresis; the Southern blot slows down evaporation.
- 10.BamHI, HindIII, and EcoRI are all examples of
- A.
Genes.
- B.
DNA ligands.
- C.
Restriction enzymes.
- D.
Southern blots.
- 11.The gel in a gel electrophoresis is
- A.
Sucrolose.
- B.
Pectrolose.
- C.
Pentarose.
- D.
Agarose.
- 12.A vector is
- A.
Used to carry foreign genes or DNA fragments.
- B.
Divided by the charge of the DNA.
- C.
Carries restriction enzymes to their restriction sites.
- D.
Only used in matching DNA samples.
- 13.________________ ends are required to create a recombinant DNA molecule.
- 14.
- A.
Are never read the same forward and backward.
- B.
End with CGT.
- C.
Begin with CGT.
- D.
Are read the same forward and backward.
- 15.____________________ is a diagram of DNA showing the cut sites of a series of restriction enzymes. (The answer may be more than one word.)
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