Restriction Enzyme Assessment

15 Questions

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Restriction Enzyme Assessment

This will test your knowledge of restriction enzymes and gel electrophoresis.


Questions and Answers
  • 1. 
    Which option BEST describes sticky ends.
    • A. 

      Sticky ends are DNA fragments that carry a higher charge than normal after they have been cleaved by restriction enzymes.

    • B. 

      Sticky ends are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other.

    • C. 

      Sticky ends are DNA fragments cleaved by a restriction enzyme so that both strands are the same length.

    • D. 

      Sticky ends are DNA fragments that attract a carbohydrate molecule to one end after being cleaved by a restriction enzyme.

  • 2. 
  • 3. 
    • A. 

      Protect the bacterium from virus attack by not allowing the virus to attach to the cell wall.

    • B. 

      Protect the bacterium from the DNA of other organisms the bacterium infects.

    • C. 

      Protect the bacterium from replicating its DNA at the wrong time.

    • D. 

      Protect the bacterium from virus attack by cutting up foreign DNA.

  • 4. 
    Restriction enzymes
    • A. 

      Bind together strands of DNA.

    • B. 

      Bind RNA fragments together.

    • C. 

      Cut DNA at specific sites.

    • D. 

      Stop transcription.

  • 5. 
    Gel electrophoresis sorts DNA molecules on the basis of their
    • A. 

      Ability to bind to mRNA.

    • B. 

      Size.

    • C. 

      Solubility in water.

    • D. 

      Solubility in the gel.

  • 6. 
    Cutting DNA with a particular restriction enzyme produces ___________  ____________ that can be separated by gel electrophoresis.
  • 7. 
    DNA ligase binds
    • A. 

      Introns together.

    • B. 

      Exons together.

    • C. 

      Polymerase to the promoter.

    • D. 

      Nucleotides together.

  • 8. 
    Based upon the picture provided, which of these are most likely to be true.
    • A. 

      The defendant may be not guilty. The recovered blood has too many bands.

    • B. 

      The defendant may be not guilty. The defendant's blood has one band not seen in the blood sample from the clothes.

    • C. 

      The defendant may be guilty. The bands from the blood on the clothes matches with the ones from the victim's blood.

    • D. 

      The defendant is may be guilty. The defendant's blood has one band not seen in the blood sample from the clothes.

  • 9. 
    • A. 

      A Southern blot is required to properly align the bands for analysis by technicians.

    • B. 

      The gel is difficult to handle; the Southern blot allows for analysis by technicians.

    • C. 

      The gel electrophoresis technique is messy; the Southern blot allows technicians to see the sample better.

    • D. 

      The gel evaporates quickly during electrophoresis; the Southern blot slows down evaporation.

  • 10. 
    BamHI, HindIII, and EcoRI are all examples of
    • A. 

      Genes.

    • B. 

      DNA ligands.

    • C. 

      Restriction enzymes.

    • D. 

      Southern blots.

  • 11. 
    The gel in a gel electrophoresis is
    • A. 

      Sucrolose.

    • B. 

      Pectrolose.

    • C. 

      Pentarose.

    • D. 

      Agarose.

  • 12. 
    A vector is
    • A. 

      Used to carry foreign genes or DNA fragments.

    • B. 

      Divided by the charge of the DNA.

    • C. 

      Carries restriction enzymes to their restriction sites.

    • D. 

      Only used in matching DNA samples.

  • 13. 
    ________________ ends are required to create a recombinant DNA molecule.
  • 14. 
    • A. 

      Are never read the same forward and backward.

    • B. 

      End with CGT.

    • C. 

      Begin with CGT.

    • D. 

      Are read the same forward and backward.

  • 15. 
    ____________________ is a diagram of DNA showing the cut sites of a series of restriction enzymes. (The answer may be more than one word.)