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Nucleic Acid Hybridization
Nucleic Acid Amplification
Nucleic Acid Sequencing
Microarrays/nanoarrays
MALDI-TOF MS
Solid support hybridization
In Situ Hybridization
In-Solution Hybridization
None of the above
Solid support hybridization
In Situ Hybridization
In-Solution Hybridization
None of the above
Most commonly method used in laboratories
Targets are detected by probes in a liquid solution
Chemiluminescence based
Target DNA or RNA is detected by probes directly in the cell or tissue
Target nucleic acid is transferred to a solid medium, probe is added to detect the target
Nucleic Acid Hybridization
Nucleic Acid Amplification
Nucleic Acid Sequencing
Microarrays / nanoarrays
MALDI-TOF MS
PCR amplicons are loaded in a vertical agarose gel
PCR amplicons are loaded in a horizontal agarose gel
An electric current is passed through the gel
Positively charged DNA will migrate from the cathode (-) to the anode (+), separated by size
Negatively charged DNA will migrate from the cathode (-) to the anode (+), separated by size
Quantitative
Qualitative
Semi-quantitative
DNA is stained and visualized under infrared light
DNA is stained and visualized under UV light
Real-time PCR (qPCR)
Reverse Transcription Polymerase Chain Reaction
Multiplex Polymerase Chain Reaction
Nested Polymerase Chain Reaction
True
False
Real-time PCR (qPCR)
Reverse Transcription Polymerase Chain Reaction
Multiplex Polymerase Chain Reaction
Nested Polymerase Chain Reaction
Uses reverse transcriptase to synthesize DNA from RNA transcripts
DNA is then amplified using conventional PCR or qPCR
PCR involving 2 or more targets which are detected simultaneously
Two consecutive PCR reactions (second reaction uses the products of the first reaction)
Produces more specific and sensitive test results
Uses reverse transcriptase to synthesize RNA from DNA transcripts
DNA is then sequenced using conventional PCR or qPCR
PCR involving 2 or more targets which are detected one at a time
Two consecutive PCR reactions (first reaction uses the products of the first reaction)
Uses reverse transcriptase to synthesize DNA from RNA transcripts
DNA is then amplified using conventional PCR or qPCR
PCR involving 2 or more targets which are detected simultaneously
Two consecutive PCR reactions (second reaction uses the products of the first reaction)
Produces more specific and sensitive test results
Uses reverse transcriptase to synthesize RNA from DNA transcripts
DNA is then sequenced using conventional PCR or qPCR
PCR involving 2 or more targets which are detected one at a time
Two consecutive PCR reactions (first reaction uses the products of the first reaction)
Uses reverse transcriptase to synthesize DNA from RNA transcripts
DNA is then amplified using conventional PCR or qPCR
PCR involving 2 or more targets which are detected simultaneously
Two consecutive PCR reactions (second reaction uses the products of the first reaction)
Produces more specific and sensitive test results
Uses reverse transcriptase to synthesize RNA from DNA transcripts
DNA is then sequenced using conventional PCR or qPCR
PCR involving 2 or more targets which are detected one at a time
Two consecutive PCR reactions (first reaction uses the products of the first reaction)
Strain typing
DNA fingerprinting
Genetic fingerprinting
All of the Above
None of the above
Strain typing
DNA fingerprinting
Genetic fingerprinting
Plasmid profile analysis
None of the above
Nucleic Acid Hybridization
Nucleic Acid Amplification
Nucleic Acid Sequencing
Microarrays/nanoarrays
MALDI-TOF MS
In high concentrations
Conjugated with fluorescent dyes
In low concentrations
Chain terminating nucleotides
In high concentrations
Conjugated with fluorescent dyes
In low concentrations
Chain terminating nucleotides
DNA fragments of varying lengths are created
Fragments are separated according to size
The fluorescent peaks in the chromatogram correspond to the terminal nucleotide
Rapid technique used to sequence shorter lengths of DNA
Template DNA to be sequenced is immobilized in a well
DNTPs are added one at a time
High-throughput and accuracy
Usually used for whole genome sequencing
DNA fragments of varying lengths are created
Fragments are separated according to size
The fluorescent peaks in the chromatogram correspond to the terminal nucleotide
Rapid technique used to sequence shorter lengths of DNA
Template DNA to be sequenced is immobilized in a well
DNTPs are added one at a time
High-throughput and accuracy
Usually used for whole genome sequencing
DNA fragments of varying lengths are created
Fragments are separated according to size
The fluorescent peaks in the chromatogram correspond to the terminal nucleotide
Rapid technique used to sequence shorter lengths of DNA
Template DNA to be sequenced is immobilized in a well
DNTPs are added one at a time
High-throughput and accuracy
Usually used for whole genome sequencing
Nucleic Acid Hybridization
Nucleic Acid Amplification
Nucleic Acid Sequencing
Microarrays/nanoarrays
MALDI-TOF MS