Molecular Methods In Microbiology

39 Questions

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Microbiology Quizzes & Trivia

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Questions and Answers
  • 1. 
    Formation of hydrogen bonds between single stranded (ss)DNA and/or RNA that are complementary to each other
    • A. 

      Nucleic Acid Hybridization

    • B. 

      Nucleic Acid Amplification

    • C. 

      Nucleic Acid Sequencing

    • D. 

      Microarrays/nanoarrays

    • E. 

      MALDI-TOF MS

  • 2. 
    Target nucleic acid is transferred to a solid medium, probe is added to detect the target
    • A. 

      Solid support hybridization

    • B. 

      In Situ Hybridization

    • C. 

      In-Solution Hybridization

    • D. 

      None of the above

  • 3. 
    Target DNA or RNA is detected by probes directly in the cell or tissue
    • A. 

      Solid support hybridization

    • B. 

      In Situ Hybridization

    • C. 

      In-Solution Hybridization

    • D. 

      None of the above

  • 4. 
    What applies to In-Solution Hybridization?
    • A. 

      Most commonly method used in laboratories

    • B. 

      Targets are detected by probes in a liquid solution

    • C. 

      Chemiluminescence based

    • D. 

      Target DNA or RNA is detected by probes directly in the cell or tissue

    • E. 

      Target nucleic acid is transferred to a solid medium, probe is added to detect the target

  • 5. 
    Simulates the in vivo DNA synthesis
    • A. 

      Nucleic Acid Hybridization

    • B. 

      Nucleic Acid Amplification

    • C. 

      Nucleic Acid Sequencing

    • D. 

      Microarrays / nanoarrays

    • E. 

      MALDI-TOF MS

  • 6. 
    Which of the choices applies to Agarose Gel Electrophoresis?
    • A. 

      PCR amplicons are loaded in a vertical agarose gel

    • B. 

      PCR amplicons are loaded in a horizontal agarose gel

    • C. 

      An electric current is passed through the gel

    • D. 

      Positively charged DNA will migrate from the cathode (-) to the anode (+), separated by size

    • E. 

      Negatively charged DNA will migrate from the cathode (-) to the anode (+), separated by size

    • F. 

      Quantitative

    • G. 

      Qualitative

    • H. 

      Semi-quantitative

    • I. 

      DNA is stained and visualized under infrared light

    • J. 

      DNA is stained and visualized under UV light

  • 7. 
    [Blank] prevents dye from fluorescing
  • 8. 
    [Blank] is activated as the probe is fragmented during the extension phase
  • 9. 
    Uses fluorescent-conjugated probes
    • A. 

      Real-time PCR (qPCR)

    • B. 

      Reverse Transcription Polymerase Chain Reaction

    • C. 

      Multiplex Polymerase Chain Reaction

    • D. 

      Nested Polymerase Chain Reaction

  • 10. 
    Fluorescence is inversely proportional with number of DNA copies synthesized
    • A. 

      True

    • B. 

      False

  • 11. 
    Allows for real-time quantification of DNA
    • A. 

      Real-time PCR (qPCR)

    • B. 

      Reverse Transcription Polymerase Chain Reaction

    • C. 

      Multiplex Polymerase Chain Reaction

    • D. 

      Nested Polymerase Chain Reaction

  • 12. 
    Which of the choices applies to Reverse Transcription Polymerase Chain Reaction?
    • A. 

      Uses reverse transcriptase to synthesize DNA from RNA transcripts

    • B. 

      DNA is then amplified using conventional PCR or qPCR

    • C. 

      PCR involving 2 or more targets which are detected simultaneously

    • D. 

      Two consecutive PCR reactions (second reaction uses the products of the first reaction)

    • E. 

      Produces more specific and sensitive test results

    • F. 

      Uses reverse transcriptase to synthesize RNA from DNA transcripts

    • G. 

      DNA is then sequenced using conventional PCR or qPCR

    • H. 

      PCR involving 2 or more targets which are detected one at a time

    • I. 

      Two consecutive PCR reactions (first reaction uses the products of the first reaction)

  • 13. 
    Which of the choices applies to Multiplex Polymerase Chain Reaction?
    • A. 

      Uses reverse transcriptase to synthesize DNA from RNA transcripts

    • B. 

      DNA is then amplified using conventional PCR or qPCR

    • C. 

      PCR involving 2 or more targets which are detected simultaneously

    • D. 

      Two consecutive PCR reactions (second reaction uses the products of the first reaction)

    • E. 

      Produces more specific and sensitive test results

    • F. 

      Uses reverse transcriptase to synthesize RNA from DNA transcripts

    • G. 

      DNA is then sequenced using conventional PCR or qPCR

    • H. 

      PCR involving 2 or more targets which are detected one at a time

    • I. 

      Two consecutive PCR reactions (first reaction uses the products of the first reaction)

  • 14. 
    Which of the choices applies to Nested Polymerase Chain Reaction?
    • A. 

      Uses reverse transcriptase to synthesize DNA from RNA transcripts

    • B. 

      DNA is then amplified using conventional PCR or qPCR

    • C. 

      PCR involving 2 or more targets which are detected simultaneously

    • D. 

      Two consecutive PCR reactions (second reaction uses the products of the first reaction)

    • E. 

      Produces more specific and sensitive test results

    • F. 

      Uses reverse transcriptase to synthesize RNA from DNA transcripts

    • G. 

      DNA is then sequenced using conventional PCR or qPCR

    • H. 

      PCR involving 2 or more targets which are detected one at a time

    • I. 

      Two consecutive PCR reactions (first reaction uses the products of the first reaction)

  • 15. 
    Differentiating different strains/species by detecting mutations in their DNA
    • A. 

      Strain typing

    • B. 

      DNA fingerprinting

    • C. 

      Genetic fingerprinting

    • D. 

      All of the Above

    • E. 

      None of the above

  • 16. 
    Uses plasmid genetic DNA instead of chromosomal DNA
    • A. 

      Strain typing

    • B. 

      DNA fingerprinting

    • C. 

      Genetic fingerprinting

    • D. 

      Plasmid profile analysis

    • E. 

      None of the above

  • 17. 
    Determining the order of nucleotides in a fragment of DNA
    • A. 

      Nucleic Acid Hybridization

    • B. 

      Nucleic Acid Amplification

    • C. 

      Nucleic Acid Sequencing

    • D. 

      Microarrays/nanoarrays

    • E. 

      MALDI-TOF MS

  • 18. 
    Most used gene to differentiate bacteria
  • 19. 
    Which applies to the dNTPs of the Sanger Sequence
    • A. 

      In high concentrations

    • B. 

      Conjugated with fluorescent dyes

    • C. 

      In low concentrations

    • D. 

      Chain terminating nucleotides

  • 20. 
    Which applies to the ddNTPs of the Sanger Sequence
    • A. 

      In high concentrations

    • B. 

      Conjugated with fluorescent dyes

    • C. 

      In low concentrations

    • D. 

      Chain terminating nucleotides

  • 21. 
    Which of the choices applies to SANGER SEQUENCING?
    • A. 

      DNA fragments of varying lengths are created

    • B. 

      Fragments are separated according to size

    • C. 

      The fluorescent peaks in the chromatogram correspond to the terminal nucleotide

    • D. 

      Rapid technique used to sequence shorter lengths of DNA

    • E. 

      Template DNA to be sequenced is immobilized in a well

    • F. 

      DNTPs are added one at a time

    • G. 

      High-throughput and accuracy

    • H. 

      Usually used for whole genome sequencing

  • 22. 
    Which of the choices applies to PYROSEQUENCING?
    • A. 

      DNA fragments of varying lengths are created

    • B. 

      Fragments are separated according to size

    • C. 

      The fluorescent peaks in the chromatogram correspond to the terminal nucleotide

    • D. 

      Rapid technique used to sequence shorter lengths of DNA

    • E. 

      Template DNA to be sequenced is immobilized in a well

    • F. 

      DNTPs are added one at a time

    • G. 

      High-throughput and accuracy

    • H. 

      Usually used for whole genome sequencing

  • 23. 
    Which of the choices applies to NEXT-GENERATION SEQUENCING ?
    • A. 

      DNA fragments of varying lengths are created

    • B. 

      Fragments are separated according to size

    • C. 

      The fluorescent peaks in the chromatogram correspond to the terminal nucleotide

    • D. 

      Rapid technique used to sequence shorter lengths of DNA

    • E. 

      Template DNA to be sequenced is immobilized in a well

    • F. 

      DNTPs are added one at a time

    • G. 

      High-throughput and accuracy

    • H. 

      Usually used for whole genome sequencing

  • 24. 
    A [Blank] detects the fluorescence in each segment
  • 25. 
    Used to profile gene expression of single/multiple organism/cells at the same time
    • A. 

      Nucleic Acid Hybridization

    • B. 

      Nucleic Acid Amplification

    • C. 

      Nucleic Acid Sequencing

    • D. 

      Microarrays/nanoarrays

    • E. 

      MALDI-TOF MS