Microbiology Module 3

108 Questions | Total Attempts: 66

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Microbiology Quizzes & Trivia

Questions and Answers
  • 1. 
    The source of variation among microoragnism is
    • A. 

      antibiotic resistance antibiotic resistance

    • B. 

      Virulence factors

    • C. 

      Sigma factors

    • D. 

      Mutation

  • 2. 
    The properties of a cell which are determined by ita DNA composition are its
    • A. 

      Phenotype

    • B. 

      Genotype

    • C. 

      Metabolism

    • D. 

      Nucleoid

  • 3. 
    Which would have the least effect on the amino acid sequence?
    • A. 

      Substitution of 1 nucleotide

    • B. 

      Deletion of 2 consecutive nucleotide

    • C. 

      Addition of 1 nucleotide

    • D. 

      Addition deletion of 3 consecutive nucleotides

    • E. 

      Substitution of 1 nucleotide AND addition of 1 nucleotide

  • 4. 
    Segments of DNA capable of moving from one area in the DNA to another are called
    • A. 

      Base analogs

    • B. 

      Intercalating agents

    • C. 

      Transposons

    • D. 

      Palindromic sequences

  • 5. 
    Transposons
    • A. 

      Are informally known as jumping genes

    • B. 

      Cause insertion mutations

    • C. 

      Cause knockout mutations

    • D. 

      Were first recognized as plants

    • E. 

      All of the choices are correct

  • 6. 
    Chemical mutagens often act by altering the
    • A. 

      Alkyl groups of nitrogen bases

    • B. 

      Nitrogen base sequence

    • C. 

      Number of binding sites on the nitrogen bases

    • D. 

      Hydrogen bonding properties of the nitrogen bases

  • 7. 
    The largest group of chemical mutagens consists of
    • A. 

      Radiation

    • B. 

      Base analogs

    • C. 

      Nitrous acid

    • D. 

      Alkylating agents

  • 8. 
    Nitrous acid most often frequently causes mutations by
    • A. 

      Substituting oxygen for hydrogen bonds

    • B. 

      Converting keto groups to amino groups

    • C. 

      Altering the bonding ratios of nitrogen bases

    • D. 

      Converting cytosine to uracil

  • 9. 
    Irradiation of cells with ultraviolet light may cause
    • A. 

      4 nucleotide to covalently bond together

    • B. 

      Thymine dymers

    • C. 

      Adenine complementary base pairing with cytosine

    • D. 

      The addition of uracil

  • 10. 
    The formation of a covalent bond between two adjacent thymines is caused by
    • A. 

      Mustard gas

    • B. 

      Alkylating agents

    • C. 

      Microwave radiation

    • D. 

      UV radiation

  • 11. 
    X-rays
    • A. 

      Have no effect on DNA

    • B. 

      Cause thymine trimers

    • C. 

      Cause single and double strand breaks

    • D. 

      Make the DNA radioactive

  • 12. 
    DNA repair mechanisms occur
    • A. 

      Only in prokaryotes

    • B. 

      Only in eukaryotes

    • C. 

      In both eukaryotes and prokaryotes

    • D. 

      In neither eukaryotes or prokaryotes

  • 13. 
    Which is not true about mismatch repair?
    • A. 

      It utilizes an endonuclease

    • B. 

      It requires DNA polymerase and DNA ligase

    • C. 

      It utilizes the state of methylation of the DNA to differentiate between strands

    • D. 

      It removes both strands in the mismatch area

  • 14. 
    Mutations
    • A. 

      May occur spontaneously

    • B. 

      Occuring at one gene are often coupled to mutation of another gene

    • C. 

      Are more likely to be killed by using antibiotics

    • D. 

      Are always harmful

    • E. 

      May occur spontaneously AND are more likely to be killed by using 2 antibiotics

  • 15. 
    Prokaryotic cell mutations can be observed very quickly because the prokaryotic chromosome is
    • A. 

      Diploid

    • B. 

      Polyploid

    • C. 

      Haploid

    • D. 

      Polysomal

  • 16. 
    The diploid character of euakryotic cells may mask the appearance of a mutation since
    • A. 

      The diploid may be a frame shift

    • B. 

      The mutation is often reversible

    • C. 

      The mutation maybe palindromic

    • D. 

      The matching chromosome may carry the dominant gene

  • 17. 
    Direct selection involves inoculating cells onto growth media on which
    • A. 

      The mutant but not the parental will grow

    • B. 

      The mutation will be reversed

    • C. 

      The nutrients necessary for mutation to occur are present

    • D. 

      The mutagen is present

  • 18. 
    Among the easiest of the mutations to isolate are those which
    • A. 

      Involve the haploid chromosome

    • B. 

      Involve antibiotic resistance

    • C. 

      Allow poplulation to be measured

    • D. 

      Use an indirect method for measurement

    • E. 

      Involve haploid chromosomes AND involve antibiotic resistance

  • 19. 
    Bacteria that have the properties of both the donor and recipient cells are the result of
    • A. 

      UV light

    • B. 

      SOS repair

    • C. 

      Frame shift mutation

    • D. 

      Genetic recombination

  • 20. 
    The mechanism by which genes are transferred into bacteria via viruses is called
    • A. 

      Ellipsis

    • B. 

      Replica plating

    • C. 

      Transformation

    • D. 

      Transduction

    • E. 

      Conjugation

  • 21. 
    In conjugation the donor cell is recognized by the presence of
    • A. 

      An F plasmid

    • B. 

      A Y chromosome

    • C. 

      Diplod chromosome

    • D. 

      An SOS response

    • E. 

      An F plasmid AND diploid chromosomes

  • 22. 
    The F plamid carries the information for
    • A. 

      Antibiotic resistance

    • B. 

      Recipient cell DNA replication

    • C. 

      The Y chromosome

    • D. 

      The sex pilus

    • E. 

      Antibiotic resistance AND the Y chromosome

  • 23. 
    Competent cells
    • A. 

      Are able to take up naked DNA

    • B. 

      Are antibiotic resistant

    • C. 

      Occur naturally

    • D. 

      Can be created in the laboratory

    • E. 

      Are able to take up naked DNA, occur naturally AND can be created in the laboratory

  • 24. 
    The material responsible for transformation was shown to be DNA by
    • A. 

      Watson and Crick

    • B. 

      Avery, MacLeod and McCarty

    • C. 

      Lederberg

    • D. 

      Stanley

  • 25. 
    In conjugation,transformation, or transduction, the recipient bacteria will take donor DNA
    • A. 

      From any source

    • B. 

      From any species of bacteria

    • C. 

      From the same species of bacteria

    • D. 

      Only through plasmids

    • E. 

      From any source AND only through plasmid