# Microbiology Lab Midterm

48 Questions | Total Attempts: 540

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A quiz to help prep for Microbio midterm on Tues. :(

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• 1.
• A.

The clearance distance between the front of the object and the uppersurface of the coverglass

• B.

The ability to discriminate between two discrete objects seperated by a minute distance

• C.

Abiility of detail to stand out against the background or other adjacent details

• D.

Individual magnifying power of the objective multiplied by that of the eyepiece

• 2.
What is the formula for the Numerical aperature (N.A.)?
• A.

N.A. = 1/n*sin(alpha)

• B.

N.A. = n*sin(alpha)

• C.

N.A. = n* sing (1/alpha)

• D.

N.A.=1/n*sin (1/alpha)

• 3.
Define Resolving Power.
• A.

The ability to discriminate between two discrete objects seperated by a minute distance

• B.

Individual magnifying power of the objective multiplied by that of the eyepiece

• C.

Abiility of detail to stand out against the background or other adjacent details

• D.

The clearance distance between the front of the object and the uppersurface of the coverglass

• 4.
Which N.A. would have a brighter image and higher resolution? N.A.= 1.25 or N.A. = 0.1?
• A.

N.A. = 1.25

• B.

N.A. =0.1

• 5.
Label all the parts of the Microscope.
• 6.
What on the microscope is used to move the objectives?
• 7.
Define Contrast.
• 8.
TRUE OR FALSE.The shorter the working distance, the lower the magnifying power.
• A.

FALSE

• B.

TRUE

• 9.
What is the purpose of the CAD (condenser aperature diaphragm) lever?
• A.

Centers the light through the specimen

• B.

Controls the width of the light beam

• C.

Controls the angle of the light beam

• D.

Centers the specimen under the light beam

• 10.
What is the purpose of the Field Diaphragm lever?
• A.

Centers the light through the specimen

• B.

Controls the width of the light beam

• C.

Controls the angle of the light beam

• D.

Centers the specimen under the light beam

• 11.
What is the purpose of the Condenser Focusing Knob?
• A.

Centers the light through the specimen

• B.

Controls the width of the light beam

• C.

Controls the angle of the light beam

• D.

Centers the specimen under the light beam

• 12.
What is the purpose of Koehler Illumination?
• A.

Increase contrast, decrease resolution, & achieve even illumination

• B.

Increase contrast, decrease resolution, & achieve uneven illumination

• C.

Decrease contrast, decrease resolution, & achieve even illumination

• D.

Decrase contrast, decrease resolution, & achieve uneven illumination

• E.

Increase contrast, increase resolution, & achieve even illumination

• 13.
List the steps of Koehler Illumination.
• 14.
Why do we use Immersion Oil instead of no oil when viewing specimens under a microscope?
• 15.
Name and describe the 3 states of media & give an example of each
• 16.
The definition of subculture is?
• A.

Transfer microbes from one media to another, ASEPTICALLY (i.e. without contamination)

• B.

Isolation of organisms for identification

• C.

Isolation of organism for counting

• 17.
Explain the different purposes between a spread plate and a streak plate.
• 18.
What shapes do spores have? Where are spores located on the cell?
• A.

Shape: spherical, circular, cylindrical; location: central, subcentral, terminal

• B.

Shape: spherical, ellipsoidal, cylindrical; location: central, terminal, subterminal

• C.

Shape: spherical, ellipsoidal, subellipsoidal; location: central, terminal, suterminal

• 19.
1. If you had 8 plates and 30 mL of water was needed per plate, how much water total is needed?2. This is equivalent to how many Liters?
• A.

24 mL, 2.4 L

• B.

240 mL, 24 L

• C.

.24 mL, .240 L

• D.

240 mL, .24 L

• 20.
Now that you have calculated that 240 mL of water is needed for 8 plates. 30 grams of powdered TSB/L of water equals _______ grams of TSB powder required.
• 21.
Describe the Morphological shapes of organisms.
• 22.
Which of the following is NOT a type of microscope?
• A.

Brightfield, fluroescent, TEM, SEM

• B.

Phase contrast, phase contract, darkfield

• C.

SEM, darkfield, brightfield, TEM

• 23.
Which of the following types of microscopes does NOT have a resolving power of 0.2 micrometeres?
• A.

Brightfield

• B.

Darkfield

• C.

Fluorescent

• D.

TEM

• 24.
What is the difference between Brightfield and Darkfield?
• 25.
Name the types of Electron microscopes and the differences between them.