Microbiology Lab Midterm

48 Questions | Total Attempts: 540

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Microbiology Lab Midterm

A quiz to help prep for Microbio midterm on Tues. :(


Questions and Answers
  • 1. 
    • A. 

      The clearance distance between the front of the object and the uppersurface of the coverglass

    • B. 

      The ability to discriminate between two discrete objects seperated by a minute distance

    • C. 

      Abiility of detail to stand out against the background or other adjacent details

    • D. 

      Individual magnifying power of the objective multiplied by that of the eyepiece

  • 2. 
    What is the formula for the Numerical aperature (N.A.)?
    • A. 

      N.A. = 1/n*sin(alpha)

    • B. 

      N.A. = n*sin(alpha)

    • C. 

      N.A. = n* sing (1/alpha)

    • D. 

      N.A.=1/n*sin (1/alpha)

  • 3. 
    Define Resolving Power.
    • A. 

      The ability to discriminate between two discrete objects seperated by a minute distance

    • B. 

      Individual magnifying power of the objective multiplied by that of the eyepiece

    • C. 

      Abiility of detail to stand out against the background or other adjacent details

    • D. 

      The clearance distance between the front of the object and the uppersurface of the coverglass

  • 4. 
    Which N.A. would have a brighter image and higher resolution? N.A.= 1.25 or N.A. = 0.1?
    • A. 

      N.A. = 1.25

    • B. 

      N.A. =0.1

  • 5. 
    Label all the parts of the Microscope.
  • 6. 
    What on the microscope is used to move the objectives?
  • 7. 
    Define Contrast.
  • 8. 
    TRUE OR FALSE.The shorter the working distance, the lower the magnifying power.
    • A. 

      FALSE

    • B. 

      TRUE

  • 9. 
    What is the purpose of the CAD (condenser aperature diaphragm) lever?
    • A. 

      Centers the light through the specimen

    • B. 

      Controls the width of the light beam

    • C. 

      Controls the angle of the light beam

    • D. 

      Centers the specimen under the light beam

  • 10. 
    What is the purpose of the Field Diaphragm lever?
    • A. 

      Centers the light through the specimen

    • B. 

      Controls the width of the light beam

    • C. 

      Controls the angle of the light beam

    • D. 

      Centers the specimen under the light beam

  • 11. 
    What is the purpose of the Condenser Focusing Knob?
    • A. 

      Centers the light through the specimen

    • B. 

      Controls the width of the light beam

    • C. 

      Controls the angle of the light beam

    • D. 

      Centers the specimen under the light beam

  • 12. 
    What is the purpose of Koehler Illumination?
    • A. 

      Increase contrast, decrease resolution, & achieve even illumination

    • B. 

      Increase contrast, decrease resolution, & achieve uneven illumination

    • C. 

      Decrease contrast, decrease resolution, & achieve even illumination

    • D. 

      Decrase contrast, decrease resolution, & achieve uneven illumination

    • E. 

      Increase contrast, increase resolution, & achieve even illumination

  • 13. 
    List the steps of Koehler Illumination.
  • 14. 
    Why do we use Immersion Oil instead of no oil when viewing specimens under a microscope?
  • 15. 
    Name and describe the 3 states of media & give an example of each
  • 16. 
    The definition of subculture is?
    • A. 

      Transfer microbes from one media to another, ASEPTICALLY (i.e. without contamination)

    • B. 

      Isolation of organisms for identification

    • C. 

      Isolation of organism for counting

  • 17. 
    Explain the different purposes between a spread plate and a streak plate.
  • 18. 
    What shapes do spores have? Where are spores located on the cell?
    • A. 

      Shape: spherical, circular, cylindrical; location: central, subcentral, terminal

    • B. 

      Shape: spherical, ellipsoidal, cylindrical; location: central, terminal, subterminal

    • C. 

      Shape: spherical, ellipsoidal, subellipsoidal; location: central, terminal, suterminal

  • 19. 
    1. If you had 8 plates and 30 mL of water was needed per plate, how much water total is needed?2. This is equivalent to how many Liters?
    • A. 

      24 mL, 2.4 L

    • B. 

      240 mL, 24 L

    • C. 

      .24 mL, .240 L

    • D. 

      240 mL, .24 L

  • 20. 
    Now that you have calculated that 240 mL of water is needed for 8 plates. 30 grams of powdered TSB/L of water equals _______ grams of TSB powder required.
  • 21. 
    Describe the Morphological shapes of organisms.
  • 22. 
    Which of the following is NOT a type of microscope?
    • A. 

      Brightfield, fluroescent, TEM, SEM

    • B. 

      Phase contrast, phase contract, darkfield

    • C. 

      SEM, darkfield, brightfield, TEM

  • 23. 
    Which of the following types of microscopes does NOT have a resolving power of 0.2 micrometeres?
    • A. 

      Brightfield

    • B. 

      Darkfield

    • C. 

      Fluorescent

    • D. 

      TEM

  • 24. 
    What is the difference between Brightfield and Darkfield?
  • 25. 
    Name the types of Electron microscopes and the differences between them.