The Toughest Trivia Quiz On Microbiology
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As we continue without preparation for the Microbiology 130 final exam, I have prepared the next exam in the series to ensure that you are adequately prepared. This quiz is made of 70 questions from different chapters. Give it a shot and see what chances you stand at acing the upcoming exam and is considered one of the toughest trivia quiz on microbiology. Give it a try!
- 1.Which nutritional requirement is “iron (Fe2+)†considered to be a part of?
- A.
The major nutritional elements
- B.
Trace elements
- C.
Organic growth factors
- D.
Vitamins
- 2.Cells that live in high salt environments are called ________.
- A.
Plasmophiles
- B.
Saltophiles
- C.
Tonophiles
- D.
Halophiles
- 3.For a mesophile, the maximum growth temperature would be ____ C.
- A.
30
- B.
37
- C.
45
- D.
60
- 4.For a thermophile, the minimum growth temperature would be ____C.
- A.
0
- B.
10
- C.
37
- D.
45
- 5.Which of these is a good method of preserving microbial cultures?
- A.
Lyophilization
- B.
Heating
- C.
Salting
- D.
Pickling
- 6.Which of these organisms would not be able to grow in water that has oxygen pumped into it (aerated)?
- A.
Obligate aerobe
- B.
Facultative aerobe
- C.
Obligate anaerobe
- D.
Aerotolerant anaerobe
- 7.Mannitol salt agar contains 7.5% salt which only allows the growth of microbes associated with the human body, such as Staphylococcus species. Furthermore, those that are able to ferment mannitol appear yellow in color. What type of medium is Mannitol salt agar?
- A.
Selective
- B.
Differential
- C.
Selective and Differential
- D.
Enrichment
- 8.Protein synthesis during lag phase is not occuring because cells are not actively dividing.
- A.
True
- B.
False
- 9.Which of these microbes is most problematic to humans?
- A.
Psychrophile
- B.
Psychrotroph
- C.
Mesophile
- D.
Thermophile
- 10.Salts and sugars work to preserve foods by creating a ____.
- A.
Lower osmotic pressure
- B.
Hypotonic environment
- C.
Lower pH
- D.
Hypertonic environment
- 11.The large intestines is an environment that lacks any oxygen. Which of the following types of organisms would most likely be found there?
- A.
Obligate aerobe
- B.
Obligate anaerobe
- C.
Microaerophile
- D.
Aerotolerant anaerobe
- 12.At which stage of bacterial growth is cell division the most rapid?
- A.
Lag
- B.
Log
- C.
Stationary
- D.
Death
- 13.Which of these microbes is most likely to grow at room temperature (25 C)?
- A.
Psychrophile
- B.
Psychrotroph
- C.
Thermophile
- D.
Hyperthermophile
- 14.____ is used if you want to study obligate anaerobic microbes.
- A.
Enrichment media
- B.
Selective media
- C.
Reducing media
- D.
Differential media
- 15.You want to quantify a culture of motile bacteria. Which method of quantifying would you use if you needed the most accurate information as soon as possible?
- A.
Plate count
- B.
MPN
- C.
Turbidity
- D.
Microscopic count
- E.
Dry weight
- 16.Motility medium is a growth agar medium that contains a low agar concentration. Cells that are motile will be able to grow throughout the medium whereas non-motile cells will only grow where they were inoculated. What type of medium is motility medium.
- A.
Differential
- B.
Selective
- C.
Differential and selective
- D.
Enrichment
- 17.Which of these organisms would not be able to grow in water that has oxygen pumped into it (aerated)?
- A.
Obligate aerobe
- B.
Facultative aerobe
- C.
Obligate anaerobe
- D.
Aerotolerant anaerobe
- 18.Most human pathogens are _________.
- A.
Psychrophiles
- B.
Mesophiles
- C.
Thermophiles
- D.
Psychrotrophs
- 19.Which method of quantifying bacterial numbers would be most effective for measuring the numbers of bacteria in drinking water, when you expect the numbers to be relatively low?
- A.
Filtration
- B.
Plate count
- C.
Direct microscopic count
- D.
Turbidity
- 20.Which of the following is a limitation of the autoclave?
- A.
Requires a long time to achieve sterilization.
- B.
Cannot inactivate viruses.
- C.
Cannot kill endospores.
- D.
Cannot be used with heat-sensitive materials.
- 21.Which of the following treatments does not yield a sterile product?
- A.
Ionizing radiation
- B.
Autoclaving
- C.
0.45 um filtration
- D.
Gamma radiation
- 22.A microbial culture contains 1 million cells. If it takes 25 minutes to kill all microbes in a culture, the DRT is ______.
- A.
25 minutes
- B.
1 million cells
- C.
Not enough information
- 23.Which one of the following would be the most likely to spoil a jar of jam in your fridge?
- A.
Eurotium, a mold
- B.
Pseudomonas, a psychrotrophic bacterium
- C.
Thibacillus, an acidophile
- D.
Streptococcus pyogenes, a human pathogen
- 24.Based upon the information provided, which of these disinfectant treatments is more effective, a higher concentration for a shorter time, or a lower concentration for a longer time?
- A.
High concentration, short time.
- B.
Low concentration, long time.
- C.
Equally effective.
- 25.In the disk-diffusion method, a ____ zone of inhibition around a filter soaked with a chemical indicates that the test bacteria is resistant to the chemical.
- A.
Small
- B.
Large
- C.
Colored
- D.
Clear
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