Microbiology #2
Microbiology quiz for test #2
- 1.What is growth?
- A.
Cell number of entire culture
- B.
Cell number of individual colony
- C.
Increase in the number of cells
- D.
Size of the cells
- 2.What are the correct steps in Binary Fission?
- A.
Chromosome Separates, Septum Forms, Cell Duplicates
- B.
Cell Duplicates, Septum Forms, Chromosome Separates
- C.
Septum Forms, Cell Duplicates, Chromosome Separate
- D.
Septum Forms, Chromosomes Separates, Cell Duplicates
- 3.Process in which a small new cell develops from the surface of an existing cell and subsequently separates from the parent cell?
- A.
Binary Fission
- B.
Mitosis
- C.
Budding
- D.
Meiosis
- 4.Phase that is slow growing and builds ATP?
- A.
Log Phase
- B.
Stationary Phase
- C.
Decline Phase
- D.
Lag Phase
- 5.Phase that is exponential?
- A.
Log Phase
- B.
Lag Phase
- C.
Stationary Phase
- D.
Death Phase
- 6.Stage in which growth continues and the cell numbers level off?
- A.
Lag Phase
- B.
Stationary Phase
- C.
Death Phase
- D.
Log Phase
- 7.The phase which is logarithmic and the number of live cells decreases?
- A.
Lag Phase
- B.
Log Phase
- C.
Death Phase
- D.
Stationary Phase
- 8.What are some ways that we measure bacterial growth?
- A.
Direct Microscopic Counts, Most Probable Number, Temperature, Oxygen Level, Filtration
- B.
Oxygen level, Filtration, Counting the Cells, Turbidity
- C.
Cloudiness, Amount of Growth on the top of the medium, Color of the growth, Thickness of the growth
- D.
Standard Plate Counts, Direct Microscopic Counts, Most Probable Number, Filtration, Turbidity
- 9.Method in which we estimate with dilutions, mix & plate each, count colonies, and multiply by dilution factor?
- A.
Serial Dilution/Standard Plate Counts
- B.
Direct Microscopic Counts
- C.
Most Probable Number
- D.
Filtration
- 10.Method that uses a hemocytometer, has cells in liquid media & trypan blue for 5 minutes, and counts live and dead cells?
- A.
Standard Plate Counts
- B.
Direct Microscopic Counts
- C.
Most Probable Number
- D.
Filtration
- 11.Method normally used in food and water sanitation studies, has low counts, uses 3 dilutions in sets of 5, and pH?
- A.
Turbidity
- B.
Direct Microscopic Counts
- C.
Most Probable Number
- D.
Filtration
- 12.Method that uses pores to collect bacteria and then grows individual colonies on media?
- A.
Turbidity
- B.
Most Probable Number
- C.
Direct Microscopic Counts
- D.
Filtration
- 13.Method that views the cloudiness visualized resulting from growth?
- A.
Turbidity
- B.
Filtration
- C.
Most Probable Number
- D.
Serial Dilution
- 14.Which is not a physical factor affecting growth?
- A.
Hydrogen
- B.
Oxygen
- C.
Moisture
- D.
PH
- 15.What is the pH of Neutrophiles?
- A.
5.4 - 8.0
- B.
0.1 - 5.4
- C.
7.0 - 11.5
- D.
1.9 - 3.7
- 16.What is the pH of acidophiles?
- A.
0.1 - 5.4
- B.
5.4 - 8.0
- C.
7.0 - 11.5
- D.
3.1 - 9.6
- 17.What is the pH of alkaliphiles?
- A.
7.0 - 11.5
- B.
0.1 - 5.4
- C.
5.4 - 8.0
- D.
1.0 - 3.7
- 18.What type of temperature do psychrophiles thrive in?
- A.
15 - 20 C
- B.
25 - 40 C
- C.
50 - 60 C
- D.
70 - 90 C
- 19.What type of temperatures do Mesophiles thrive in?
- A.
25 - 40 C
- B.
15 - 20 C
- C.
50 - 60 C
- D.
70 - 80 C
- 20.Which is an example of an obligate aerobe?
- A.
Pseudomonas
- B.
Tetani
- C.
Botulinum
- D.
Bacteroides
- 21.Which is an example of an obligate anaerobe?
- A.
Botulinum
- B.
Pseudomonas
- C.
Staph
- D.
Coccus
- 22.Moisture requires the presence of what?
- A.
H20
- B.
Oxygen
- C.
Hydrogen
- D.
Helium
- 23.What maintains enzyme conformation?
- A.
Osmotic Pressure
- B.
Hydrostatic Pressure
- C.
Radiation
- D.
Temperature
- 24.What prevents dehydration and rupture and is a common feature of halophiles?
- A.
Hydrostatic Pressure
- B.
Osmotic Pressure
- C.
Oxygen
- D.
Temperature
- 25._____________ and ___________ are two types of radiation and they can cause _______ mutations?
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