Anton van Leeuwenhoek
Fixing details of the nucleus.
Fixing details of collagen.
Fixing muscle tissue.
I give up!
None of the bove.
He extensively revised the first simple microscope and supported Leeuwenhoek's findings.
He invented the first simple microscope, which helped Galileo develop the telescope.
He improved the magnification of the microscope to x1250.
He revised the first simple microscope which used electron beams as his light source.
The Sliding microtome sections whole organs and bones, while the Rotary microtome moves tissue closer to the blade with a rotating wheel.
The Rotary microtome sections whole organs and bone, while the Sliding microtome moves tissue closer to the blade with a rotating wheel.
Knott and Ruska
Scleiden and Scwhann
None of the above
See protein structures of organelles and membranes
Distinguish objects that are 10 angstroms apart from one another
Distinguish objects that are 10^(-10) meters apart from one another
All of the above.
Nachet developed a small handheld microtome.
Spencer Lens Co. developed a CO2 canister attachment to freeze tissues right on the microtome.
Linderstrom-Lang a small handheld microtome.
Baush & Lomb developed the sliding microtome.
All of the above.
Decalcification of bone.
Defferentiation of muscle and collagen stains.
Enhancing nuclear details.
Technicon invented the first automated OPEN tissue processor.
McKormic Ind. invented "Tissue Tek" embedding units.
Sakura and Miles Co. invented the first CLOSED tissue processor with computer programming.
Feather Industries invented the first modern day disposable blades, replacing tweazers and steel blades.
A, B, C, and D.
Spencer Lens Co.
What's a microtome?
Stilling developed frozen tissue sectioning.
Quekett developed decalcification of fixed bone with HCl.
Born and Stickler developed paraffin(routine today) for tissue embedding.
Duval developed celloidin for excellent processing of brain tissues.
None of the Above.
1: Cambridge Instrument Co.large sledge microtome, 2: Sledge chain driven microtome by Leitz, 3: Spencer Freezing Microtome, 4: Zimmerman Rotary Microtome, 5: Cambridge “Rocking” Microtome
1: Cambridge “Rocking” Microtome, 2: Zimmerman Rotary Microtome, 3: Spencer Freezing Microtome, 4: Sledge chain driven microtome by Leitz, 5: Cambridge Instrument Co.large sledge microtome
They all look the same!
Hematoxylin: insect (blue)
Carmine: logwood tree (red)
Saffron: plant (yellow)
A and B
B and C
Perkins was a chemist in the textile industry.
Perkins synthesized the first aniline-based dyes.
The two dyes Perkins developed are Analine blue and Alcian blue
The synthetic dyes Perkins developed are widely used in histology.
Abnormal fibrillar deposits & Mucin secreting tumors.
Pneumonia & Amyloidosis
Glycogen storage disease & Disease of kidney glomeruli.
Hematoxylin & Eosin
Methylene Blue & Eosin
Hematoxylin & Congo Red
Apple green for the big group vs. red apple for Gram + /positive
Blue for the big group vs. Red for Gram + /positive
Red for the big group vs. Blue for Gram + /positive
Snap freezing preserves enzyme activity. Alkaline phosphate helps release ATP energy in muscle.
Snap freezing increases enzyme activity. Alkaline phosphate
Snap freezig produces aerobic energy (sugar breakdown in the mitochondria). Alkaline phosphate produces anaerobic energy (glycogen breakdown/glycolysis).
Both diagnose enzyme storage difficiencies.
Fromman: nerve axons Maresc: reticulin fibers of liver and spleen
Gossett and Masson: carcinoid tumors of bowel and colon Gomori methenamine silver: carbohydrates
Gomori methenamine silver: fungus Grocott methenamine silver: carbohydrates