The History Of Histology Ppt (Exam 1)

24 Questions | Total Attempts: 206

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The History Of Histology Ppt (Exam 1)

This Quiz is created by a student of HLAB 1401 at HCCS Coleman. It reviews the Histotechnology student's understanding of the history and development of histotech procedures. This Quiz is helpful for HLAB 1401 Exam #1.


Questions and Answers
  • 1. 
     This simple, hand-held microscope was invented by a man known as the "Father of Microscopy," who studied bacteria, yeast, protozoans and RBCs with it.  What is his name?
    • A. 

      Robert Hooke

    • B. 

      Anton van Leeuwenhoek

    • C. 

      Friedrich Henle

    • D. 

      Johannes Muller

  • 2. 
    What did Clark find that the compound fixative - alcohol and acetic acid - was good at?
    • A. 

      Fixing details of the nucleus.

    • B. 

      Fixing details of collagen.

    • C. 

      Fixing muscle tissue.

    • D. 

      I give up!

  • 3. 
    A fixative preserves tissue. Who accidentally discovered the fixative properties of formalin when parts of his hands became stiff?
    • A. 

      Blum

    • B. 

      Clark

    • C. 

      Hooke

    • D. 

      None of the bove.

  • 4. 
    Which is not one of the three mentioned Simple Fixatives?
    • A. 

      Alcohol

    • B. 

      Potassium dichromate

    • C. 

      Formalin

    • D. 

      Chromic acid

  • 5. 
    Why is Robert Hooke known as the "British father of microscopy"?
    • A. 

      He extensively revised the first simple microscope and supported Leeuwenhoek's findings.

    • B. 

      He invented the first simple microscope, which helped Galileo develop the telescope.

    • C. 

      He improved the magnification of the microscope to x1250.

    • D. 

      He revised the first simple microscope which used electron beams as his light source.

  • 6. 
    What is special about Bausch & Lomb's Sliding microtome and what is special about Bausch & Lomb's Rotary microtome? 
    • A. 

      The Sliding microtome sections whole organs and bones, while the Rotary microtome moves tissue closer to the blade with a rotating wheel.

    • B. 

      The Rotary microtome sections whole organs and bone, while the Sliding microtome moves tissue closer to the blade with a rotating wheel.

    • C. 

      Umm.. ?

  • 7. 
    Who invented the electron microscope?
    • A. 

      Knott and Ruska

    • B. 

      Mayer

    • C. 

      Scleiden and Scwhann

    • D. 

      None of the above

  • 8. 
    The Electron microscope used electron beams as its light source, giving it the ability to:
    • A. 

      See protein structures of organelles and membranes

    • B. 

      Distinguish objects that are 10 angstroms apart from one another

    • C. 

      Distinguish objects that are 10^(-10) meters apart from one another

    • D. 

      All of the above.

  • 9. 
    Which answer(s) is(are) false?*Hint: find out who developed the first cryostat (a microtome inside a freezer).
    • A. 

      Nachet developed a small handheld microtome.

    • B. 

      Spencer Lens Co. developed a CO2 canister attachment to freeze tissues right on the microtome.

    • C. 

      Linderstrom-Lang a small handheld microtome.

    • D. 

      Baush & Lomb developed the sliding microtome.

    • E. 

      All of the above.

  • 10. 
    What was Bouin's fixative - Picric acid, Formaldehyde, and Acetic acid - good for?
    • A. 

      Decalcification of bone.

    • B. 

      Defferentiation of muscle and collagen stains.

    • C. 

      Enhancing nuclear details.

    • D. 

      Nothing.

  • 11. 
    The manufacturers Technicon, Sakura and Miles Co, McKormic Ind., and Feather Industrires all contributed to histotech procedures.  Who did what?
    • A. 

      Technicon invented the first automated OPEN tissue processor.

    • B. 

      McKormic Ind. invented "Tissue Tek" embedding units.

    • C. 

      Sakura and Miles Co. invented the first CLOSED tissue processor with computer programming.

    • D. 

      Feather Industries invented the first modern day disposable blades, replacing tweazers and steel blades.

    • E. 

      A, B, C, and D.

  • 12. 
    Who invented the first microtome in order to study timber?
    • A. 

      John Hill

    • B. 

      Nachet

    • C. 

      Spencer Lens Co.

    • D. 

      What's a microtome?

  • 13. 
    Each scientist is paired with his contribution to histotech procedures.  Which is false?
    • A. 

      Stilling developed frozen tissue sectioning.

    • B. 

      Quekett developed decalcification of fixed bone with HCl.

    • C. 

      Born and Stickler developed paraffin(routine today) for tissue embedding.

    • D. 

      Duval developed celloidin for excellent processing of brain tissues.

    • E. 

      None of the Above.

  • 14. 
    There are 5 microtome images in the powerpoint.  Identify their names in the sequence given.1:   2: 3:                    4:  5:
    • A. 

      1: Cambridge Instrument Co.large sledge microtome, 2: Sledge chain driven microtome by Leitz, 3: Spencer Freezing Microtome, 4: Zimmerman Rotary Microtome, 5: Cambridge “Rocking” Microtome

    • B. 

      1: Cambridge “Rocking” Microtome, 2: Zimmerman Rotary Microtome, 3: Spencer Freezing Microtome, 4: Sledge chain driven microtome by Leitz, 5: Cambridge Instrument Co.large sledge microtome

    • C. 

      They all look the same!

  • 15. 
    Which Natural Dye(s) is(are) not matched with its(their) correct source of origin and color?
    • A. 

      Hematoxylin: insect (blue)

    • B. 

      Carmine: logwood tree (red)

    • C. 

      Saffron: plant (yellow)

    • D. 

      A and B

    • E. 

      B and C

  • 16. 
    Which is not true about Perkins?
    • A. 

      Perkins was a chemist in the textile industry.

    • B. 

      Perkins synthesized the first aniline-based dyes.

    • C. 

      The two dyes Perkins developed are Analine blue and Alcian blue

    • D. 

      The synthetic dyes Perkins developed are widely used in histology.

  • 17. 
    Which of the following is not a synthetic dye?
    • A. 

      Aniline blue

    • B. 

      Hematoxylin

    • C. 

      Methylene blue

  • 18. 
    The MacManus PAS stains red in the diagnosis of two different diseases.  What are the two diseases?
    • A. 

      Abnormal fibrillar deposits & Mucin secreting tumors.

    • B. 

      Pneumonia & Amyloidosis

    • C. 

      Glycogen storage disease & Disease of kidney glomeruli.

  • 19. 
    In order to destinguish between the cell's nucleus and cytoplasm, two dyes are used - one stains the nucleus blue, while the other stains the cytoplasm pink.  What are these two dyes called?
    • A. 

      Hematoxylin & Eosin

    • B. 

      Methylene Blue & Eosin

    • C. 

      Hematoxylin & Congo Red

    • D. 

      I'm confused..

  • 20. 
    In immunohistochemistry, tagged antibodies are used to localize specific tissue antigens.    Coons, Creech, and Jones used a flourochrome for use with a floursecent microscope, while Nakane and Pierce used an enzyme for use with a light microscope.  True or Flase?
    • A. 

      True

    • B. 

      False

  • 21. 
    What word did Mayer coin that also meant "web"?
    • A. 

      "heme"

    • B. 

      "microtome"

    • C. 

      "histos"

    • D. 

      "angstrom"

  • 22. 
    The Ziehl Neelsen carbol fuschin stain & the Bacterial Gram stain are both used to detect infectious microbes.  Tissues with acid-fast organisms, TB, leprosy, and Gram - /negative bacteria stain differently from Gram + /positive bacteria.  What color should the first group stain and what color should Gram + /positive stain?
    • A. 

      Apple green for the big group vs. red apple for Gram + /positive

    • B. 

      Blue for the big group vs. Red for Gram + /positive

    • C. 

      Red for the big group vs. Blue for Gram + /positive

  • 23. 
    In muscle tissue, enzyme histochemistry is utilized.  Why should you snap freeze muscle tissue with liquid nitrogen?  And why should you treat muscle tissue with alkaline phosphate like Takamtsu and Gomori did?
    • A. 

      Snap freezing preserves enzyme activity. Alkaline phosphate helps release ATP energy in muscle.

    • B. 

      Snap freezing increases enzyme activity. Alkaline phosphate

    • C. 

      Snap freezig produces aerobic energy (sugar breakdown in the mitochondria). Alkaline phosphate produces anaerobic energy (glycogen breakdown/glycolysis).

    • D. 

      Both diagnose enzyme storage difficiencies.

  • 24. 
    There are many Silver impregnation techniques (stains black) that serve different purposes.  Which two Silver impregnation techniques are mispaired with their primary puposes?  
    • A. 

      Fromman: nerve axons Maresc: reticulin fibers of liver and spleen

    • B. 

      Gossett and Masson: carcinoid tumors of bowel and colon Gomori methenamine silver: carbohydrates

    • C. 

      Gomori methenamine silver: fungus Grocott methenamine silver: carbohydrates