Trivia Quiz On Molecular Biology Exam!
(15).jpg "Trivia Quiz On Molecular Biology Exam!")
- 1.Which of the following statements is NOT true concerning homologous recombination?
- A.
It is a reciprocal event.
- B.
It can be DNA- biomolecular.
- C.
It can be intra DNA recombination.
- D.
Only one cross over event can occur.
- E.
It results in genetic variation.
- 2.Which of the following techniques was the key in demonstrating the presynaptic and synapsis steps of homologous recombination?
- A.
Southern Blot
- B.
Exonuclease digestion
- C.
Electron microscopy
- D.
S1 nuclease mapping
- E.
DNA footprinting
- 3.Which of the following enzymes cannot catalyze the formation of a phosphodiester bond?
- A.
Endonuclease
- B.
Ligase
- C.
None of the choices are correct
- D.
DNA polymerase
- E.
RNA polymerase
- 4.Which of the following repair mechanisms is a damage bypass mechanism, not an actual repair mechanism?
- A.
DNA photolyase
- B.
Mismatch repair
- C.
Recombination repair
- D.
Base excision repair
- E.
Nonhomologous end joining
- 5.Experiments conducted by Frederick Griffiths laid the foundation for
- A.
Elucidation of tRNA structure.
- B.
The virulence of S. pnuemoniae.
- C.
DNA as the genetic material.
- D.
Elucidation of mRNA structure.
- E.
Discovery of the capsule on S. pneumonia.
- 6.Which of the following is not part of the E. coli primosome?
- A.
DnaB
- B.
DnaA
- C.
TerE
- D.
DnaG
- E.
OriC
- 7.Which of the following techniques is not used to quantify the level of gene expression?
- A.
Northern blotting
- B.
S1 mapping
- C.
RNase protection
- D.
Southern blotting
- E.
Nuclear run-off
- 8.Rapid purification of proteins based on size can best be done by using
- A.
Gel filtration
- B.
Cation-exchange chromotography
- C.
Ion-exchange chromotography
- D.
Native gel electrophoresis
- E.
2-D gel electrophoresis
- 9.A primose can be defined as
- A.
A collection of RNA primers.
- B.
An area of DNA near the replication fork waiting to be replicated.
- C.
A collection of proteins needed for synthesis of primers for replicating DNA.
- D.
The enzyme that synthesizes primers for M13 phage replication.
- E.
A DNA molecule that is primed and ready for replication.
- 10.West and colleagues used gel mobility shift assays to demonstrate that could bind to and resolve Holliday junctions
- A.
RecA
- B.
RecB
- C.
RuvC
- D.
RuvA
- E.
RuvB
- 11.Rapid cooling of DNA following heating at high temperature will cause
- A.
The strands to remain separated.
- B.
The strands to break.
- C.
The double helix to form rapidly.
- D.
Mismatching between the strands.
- E.
Rapid renaturation of the strands.
- 12.Which of the following has no reverse transcriptase gene associated with it?
- A.
Tn3
- B.
L1
- C.
Ty
- D.
Copia
- E.
HIV
- 13.Which of the following is likely yo contribute to a shift in the reading frame in an mRNA molecule?
- A.
Removal of six bases
- B.
Removal of triplet code
- C.
Removal of a single base
- D.
None of the choices below are correct
- E.
Removal of nine bases
- 14.Thea early notion of one-gene/one enzyme was not true because of which of the following?
- A.
An enzyme can be composed of more than one polypeptide, many genes contain the information for making polypeptides that are not enzymes and the end products of some genes are not polypeptides.
- B.
The end products of some genes are not polypeptides
- C.
An enzyme can be more than one polypeptide
- D.
Many genes contain information for making polypeptides that are not enzymes
- 15.Select the component that is mmost critical to the function of a plasmid
- A.
Ligase
- B.
Origin of replication
- C.
EcoRI site
- D.
Ampicillian resistance
- E.
Tetracycline resistance
- 16.One approach to prevent religation of the ends of a restricted plasmid DNA is to
- A.
Treat the plasmid with Bam HI to create gaps
- B.
Treat the plasmid with alkaline phosphatase ti remove phosphate for the sticky ends
- C.
Cut the plasmid with RNase
- D.
Cleave the plasmid with DNase
- 17.A major function of reporter gene assays is to
- A.
Measure transcriptional levels
- B.
Monitor CAT activity
- C.
Measure replication
- D.
Measure mutation rates of a gene
- 18.A new insulin gene was isolated and a study was planned to look at the function of the promoter region which is the BEST choice for this study
- A.
Immunoblotting
- B.
PCR
- C.
Report gene assay
- D.
All best
- 19.Which of the following would probably would not be a characteristic of the Tn3 transposon?
- A.
OriT site
- B.
Resolvase gene
- C.
Inverted repeat sequences
- D.
Transposase gene
- 20.Which of the following is true about wild type organisms?
- A.
They are always found in the wild
- B.
They carry no recessive genes
- C.
They are referred to as standard types
- D.
None of the choices are correct
- 21.Which of the statements is not true concerning DNA replication in prokaryotes?
- A.
There is one active origin of replication
- B.
DNA replication is RNA dependent
- C.
DNA replication is semiconservative
- D.
DNA replication requires only one enzyme
- 22.The ability of two polynucleotide strands to hybridize is based on which of the following principles?
- A.
Presence of 3' and 5' ends
- B.
Complementary base pairing
- C.
Double helical nature of DNA
- D.
Presence of covalent bonds
- 23.Disadvantage of using a prokaryotic expression system for eukaryotic proteins ts that the proteins are
- A.
Improperly folded
- B.
Over expressed
- C.
Highly soluble
- D.
Heavily glycosylated
- 24.Construction of an expression vector, which of the following would you include in order to stimulate a high level of RNA synthesis
- A.
His region
- B.
GFP
- C.
Amp gene
- D.
T7 phage promoter
- E.
Origin of replication
- 25.The messenger hypothesis states that
- A.
New ribosome are made to transmit new genetic information
- B.
Messenger RNAs carry genetic information to ribosomes
- C.
Ribosomal RNAs carry information for making proteins
- D.
None of the choices are correct
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