Bsl-3 Quiz - Staff
All staff members using the BSL-3 must pass this quiz prior to working in the BSL-3.
Questions and Answers
- 1.
If you are exposed to blood or OPIM that has been contaminated with HBV, your risk of acquiring serological evidence of an infection is:
- Slightly less than_____%
Explanation
If you are exposed to blood or OPIM (other potentially infectious materials) that has been contaminated with HBV (Hepatitis B virus), your risk of acquiring serological evidence of an infection is slightly less than 40%. This means that there is a high chance of acquiring an infection, but it is not guaranteed. Taking precautionary measures such as using personal protective equipment and following proper infection control protocols can help reduce the risk further.Rate this question:
- 2.
If you are exposed to blood or OPIM that has been contaminated with HCV, your risk of acquiring serological evidence of an infection is:
- ______%
Explanation
The answer 2% suggests that if you are exposed to blood or OPIM contaminated with HCV (Hepatitis C Virus), there is a 2% chance that you will acquire serological evidence of an infection. This means that out of every 100 people exposed to contaminated blood or OPIM, approximately 2 individuals will show signs of being infected with HCV.Rate this question:
- 3.
If you are exposed to blood or OPIM that has been contaminated with HIV, your risk of acquiring serological evidence of an infection is:
- Slightly less than ____%
Explanation
If you are exposed to blood or OPIM (other potentially infectious materials) that has been contaminated with HIV (Human Immunodeficiency Virus), your risk of acquiring serological evidence of an infection is slightly less than 0.3%. This means that there is a very low chance of getting infected with HIV if you come into contact with contaminated blood or OPIM. It is important to take proper precautions and follow safety protocols to minimize the risk of exposure to HIV and other bloodborne pathogens.Rate this question:
- 4.
What is an effective treatment/prevention method against HBV?
- A.
Antibiotics
- B.
HBV Vaccine
- C.
Alcohol
Correct Answer
B. HBV VaccineExplanation
The most effective treatment and prevention method against HBV (Hepatitis B Virus) is the HBV Vaccine. This vaccine stimulates the body's immune system to produce antibodies against the virus, providing long-term protection against infection. Antibiotics are not effective against viral infections like HBV, as they only target bacterial infections. Alcohol does not have any direct effect on the prevention or treatment of HBV.Rate this question:
-
- 5.
The needlestick hotline (415) 353-7842 provides expedited access to post-exposure treatment, follow up and consulting.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The given statement is true. The needlestick hotline (415) 353-7842 is a resource that provides quick access to post-exposure treatment, follow-up care, and consulting for individuals who have experienced needlestick injuries. This hotline is designed to ensure that individuals receive prompt medical attention and support after such incidents.Rate this question:
-
- 6.
When do you call the needlestick hotline?
- A.
Immediately, because the effectiveness of the treatment is time sensitive.
- B.
When you are done with your experiment.
- C.
The next day after a sleepless night.
Correct Answer
A. Immediately, because the effectiveness of the treatment is time sensitive.Explanation
It is important to call the needlestick hotline immediately because the effectiveness of the treatment for a needlestick injury is time-sensitive. The sooner the appropriate treatment is initiated, the better the chances of preventing any potential infections or complications. Delaying the call or waiting until the next day can result in a delay in receiving necessary medical attention, which can increase the risk of infection or other adverse outcomes.Rate this question:
-
- 7.
It is OK to give access to the BSL-3 to anyone who asks.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Giving access to the BSL-3 (Biosafety Level 3) to anyone who asks is not okay. BSL-3 is a high-containment facility designed for work with potentially dangerous pathogens. It requires specialized training, proper protocols, and strict adherence to safety measures. Allowing unrestricted access to such a facility would pose a significant risk to both the individuals entering the facility and the surrounding environment. Therefore, it is not appropriate to grant access to the BSL-3 to anyone who asks.Rate this question:
-
- 8.
Minors can access the BSL-3 if accompanied by an adult.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Minors cannot access the BSL-3 even if accompanied by an adult.Rate this question:
-
- 9.
Who is responsible for the safety of maintenance personnel or anyone else that you have given access to the BSL-3?
- A.
The person who gives him/her access
- B.
The BSL-3 Manager
- C.
The Facilities Department
Correct Answer
A. The person who gives him/her accessExplanation
The person who gives maintenance personnel or anyone else access to the BSL-3 is responsible for their safety. This includes ensuring that they have proper training, knowledge of safety protocols, and necessary protective equipment. It is their responsibility to oversee and supervise the individuals while they are in the BSL-3 to ensure their safety and the safety of others in the facility.Rate this question:
-
- 10.
It is OK to use a cell phone or an iPod in the BSL3 if a user removes their outer gloves when holding it.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Using a cell phone or an iPod in a BSL3 facility is not allowed, even if the user removes their outer gloves. BSL3 facilities are designed for working with potentially dangerous pathogens, and strict protocols are in place to prevent any contamination or release of these pathogens. Allowing personal electronic devices in the BSL3 facility could pose a risk of contamination and compromise the safety of the facility and its occupants. Therefore, the statement is false.Rate this question:
-
- 11.
What is the personal protective equipment for the BSL3?
- A.
Double gloves, gown and goggles
- B.
Gloves and lab coat
Correct Answer
A. Double gloves, gown and gogglesExplanation
Personal protective equipment (PPE) for BSL3 (Biosafety Level 3) includes double gloves, a gown, and goggles. This level of biosafety requires a higher level of protection due to the handling of potentially hazardous biological agents. Double gloves provide an extra layer of protection for the hands, a gown helps cover the body, and goggles protect the eyes from any potential splashes or aerosols. This combination of PPE helps minimize the risk of exposure and ensures the safety of individuals working in BSL3 environments.Rate this question:
-
- 12.
To better notice potential defects or tears in outer gloves, the inside gloves should be of a darker color.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The statement suggests that using inside gloves of a darker color can help in identifying any defects or tears in the outer gloves more easily. This is because a darker color would create a higher contrast, making it easier to spot any irregularities or damages on the outer gloves. Therefore, the statement is true as it provides a practical solution to enhance the detection of potential defects or tears in outer gloves.Rate this question:
-
- 13.
What are the two liquids used as decontaminants in the BSL-3?
- A.
Bleach
- B.
Soapy water
- C.
Ethanol 70
- D.
Antibiotic gel
Correct Answer(s)
A. Bleach
C. Ethanol 70Explanation
Bleach and Ethanol 70 are the two liquids used as decontaminants in the BSL-3. Bleach is a powerful disinfectant that can kill a wide range of microorganisms. It is commonly used to sanitize surfaces and equipment in laboratories. Ethanol 70, also known as isopropyl alcohol, is another effective decontaminant. It has strong antimicrobial properties and is often used to disinfect medical instruments and surfaces. Both bleach and ethanol 70 are crucial in maintaining a sterile and safe environment in BSL-3 laboratories.Rate this question:
-
- 14.
Double decontamination of your wastes is recommended for highly infectious material. To do so, add bleach from the squirt bottle for a final concentration of 10%, decontaminate for 30 minutes, aspirate all liquids, discard in the appropriate dry waste container
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
Double decontamination of highly infectious material is recommended to ensure thorough disinfection. The process involves adding bleach to achieve a final concentration of 10%, decontaminating for 30 minutes, aspirating all liquids, and discarding the waste in the appropriate dry waste container. This multi-step approach helps to minimize the risk of contamination and ensure proper disposal of the infectious material. Therefore, the statement is true.Rate this question:
-
- 15.
Once the bleach bucket is filled with pipettes,
- A.
Wait 30 minutes before draining the pipettes and discarding them in the solid waste containers
- B.
Leave it aside in the BSC and bring a new bleach bucket filled half way with 10% bleach
Correct Answer
B. Leave it aside in the BSC and bring a new bleach bucket filled half way with 10% bleach -
- 16.
Order the step of the to clean a biohazard spill a. Add 10% bleach to soak the spill control pad and let it react for 30 minutes. b. Place the spill control pad over liquid to absorb and control the spread of liquid. c. Carefully remove pad, place into doubled autoclave bags. d. Dispose of clean-up materials appropriately. e. Decontaminate all exposed surfaces with 10% bleach disinfectant and rinse with 70% ethanol. f. Remove personnel protective equipment (PPE), wash hands, and exit BSL-3 laboratory to the anteroom.
- A.
A, b, c, d, e, and f
- B.
B, a, c, d, e, and f
Correct Answer
B. B, a, c, d, e, and fExplanation
The correct order of steps to clean a biohazard spill is b, a, c, d, e, and f. First, the spill control pad should be placed over the liquid to absorb and control the spread of the liquid. Then, 10% bleach should be added to soak the spill control pad and left to react for 30 minutes. Next, the pad should be carefully removed and placed into doubled autoclave bags. After that, the clean-up materials should be disposed of appropriately. All exposed surfaces should be decontaminated with 10% bleach disinfectant and rinsed with 70% ethanol. Finally, personnel protective equipment (PPE) should be removed, hands should be washed, and the BSL-3 laboratory should be exited to the anteroom.Rate this question:
-
- 17.
What do you do if you want to ship or transport non-inactivated samples out of the BSL3?
- A.
Read the UCSF Shipment of Biological Materials Manual.
- B.
Have a current bloodborne pathogen training certification from OEH&S.
- C.
A current “Safe Shipping of Biohazards” training
- D.
Have the approval of the BSL3 Lab Manager and the user's PI.
Correct Answer(s)
A. Read the UCSF Shipment of Biological Materials Manual.
B. Have a current bloodborne pathogen training certification from OEH&S.
C. A current “Safe Shipping of Biohazards” training
D. Have the approval of the BSL3 Lab Manager and the user's PI.Explanation
To ship or transport non-inactivated samples out of the BSL3, it is necessary to follow the guidelines provided in the UCSF Shipment of Biological Materials Manual. Additionally, one must have a current bloodborne pathogen training certification from OEH&S and a current "Safe Shipping of Biohazards" training. Finally, it is important to have the approval of both the BSL3 Lab Manager and the user's PI. These steps ensure that proper protocols are followed to maintain safety and prevent any potential risks during transportation.Rate this question:
-
- 18.
You need to get the approval from the BSL-3 Manager to bring new items into the BSL-3.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
In order to bring new items into the BSL-3, it is necessary to obtain approval from the BSL-3 Manager. This implies that there are certain protocols and regulations in place for the introduction of new items into the BSL-3 facility. Without the approval from the BSL-3 Manager, it would not be permitted to bring in new items, highlighting the importance of obtaining the necessary authorization before introducing any items into the BSL-3 environment.Rate this question:
-
- 19.
Is it OK to leave some liquid in the solid waste container? Why?
- A.
No, it could leak on the floor. Then it is slippery.
- B.
Yes, the plastic bags contain it.
- C.
Yes, a small amount of liquid is not a danger.
Correct Answer
A. No, it could leak on the floor. Then it is slippery.Explanation
Leaving liquid in the solid waste container could lead to leakage, which can cause the floor to become slippery. This poses a safety hazard as people may slip and fall. Therefore, it is not okay to leave liquid in the container.Rate this question:
-
- 20.
The lid of the sharps containers must be clicked on it before use.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The lid of sharps containers must be clicked on before use to ensure that it is securely closed and will not open accidentally. This is important for safety reasons, as sharps containers are used to dispose of sharp objects such as needles and blades that can cause injury. By clicking the lid on, it prevents any potential spillage or exposure to these sharp objects, reducing the risk of accidents or infections.Rate this question:
-
- 21.
There are 5 waste containers around the BSC:
- bleach bucket
- aspiration jar
- small solid waste
- large solid waste
- sharps containers
- A.
Emptied eppendorf tubes
- B.
Tips
- C.
Pipettes
- D.
Liquid
- E.
Recapped falcon tubes
- F.
96v plates without liquid
- G.
T175 previously treated for 30 minutes with 10% bleach
- H.
Ultracentrifugation tubes
Correct Answer(s)
C. Pipettes
D. LiquidExplanation
A bleach bucket is used to dispose of pipettes and liquids.Rate this question:
- 22.
There are 5 waste containers around the BSC:
- bleach bucket
- aspiration jar
- small solid waste
- large solid waste
- sharps containers
- A.
Emptied eppendorf tubes
- B.
Tips
- C.
96v plates without liquid
- D.
Liquid
- E.
Recapped falcon tubes
- F.
Ultracentrifugation tubes that have no cap
- G.
T175 previously treated for 30 minutes with 10% bleach
- H.
Pipettes
Correct Answer(s)
A. Emptied eppendorf tubes
B. Tips
C. 96v plates without liquid
F. Ultracentrifugation tubes that have no capExplanation
Sharps containers are specifically designed for the disposal of sharp objects such as needles, syringes, scalpels, and other medical instruments that may cause injury or puncture wounds. In this case, the items listed in the answer options are all sharp objects that should be disposed of in a sharps container for safety purposes.Rate this question:
- 23.
There are 5 waste containers around the BSC:
- bleach bucket
- aspiration jar
- small solid waste
- large solid waste
- sharps containers
- A.
Pipettes
- B.
Recapped falcon tubes
- C.
96v plates without liquid
- D.
Ultracentrifugation tubes
- E.
Tips
- F.
Liquid
- G.
Flasks previously treated for 30 minutes with 10% bleach
- H.
Emptied eppendorf tubes
- I.
Recapped Flasks
Correct Answer(s)
B. Recapped falcon tubes
G. Flasks previously treated for 30 minutes with 10% bleach
I. Recapped FlasksExplanation
The items that go into a large solid waste container are recapped falcon tubes, flasks previously treated for 30 minutes with 10% bleach, and recapped flasks.Rate this question:
- 24.
There are 5 waste containers around the BSC:
- bleach bucket
- aspiration jar
- small solid waste
- large solid waste
- sharps containers
- A.
Ultracentrifugation tubes
- B.
T175 previously treated for 30 minutes with 10% bleach
- C.
96v plates without liquid
- D.
Pipettes
- E.
Tips
- F.
Liquid
- G.
Recapped falcon tubes
- H.
Emptied eppendorf tubes
Correct Answer
F. LiquidExplanation
An aspiration jar is used to collect and dispose of liquid waste. Therefore, any items that contain liquid, such as pipettes, tips, liquid in tubes, recapped falcon tubes, and emptied eppendorf tubes, should be placed in the aspiration jar.Rate this question:
- 25.
Removing the maximum amount of bleach from the waste being autoclaved avoids corrosion of the autoclave.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
Removing the maximum amount of bleach from the waste being autoclaved helps prevent corrosion of the autoclave. Bleach is a strong oxidizing agent that can cause damage to the autoclave chamber and components over time. By removing as much bleach as possible from the waste, the risk of corrosion is minimized, ensuring the longevity and proper functioning of the autoclave equipment.Rate this question:
-
- 26.
You are considered an amazing BSL-3 user if you shoot pipette tips into the bleach bucket at a 99% success rate.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
The statement suggests that being considered an amazing BSL-3 user is determined by shooting pipette tips into the bleach bucket at a 99% success rate. However, this is not a valid criterion for determining someone's proficiency as a BSL-3 user. Being skilled at shooting pipette tips into a bleach bucket does not necessarily reflect one's overall competence in handling hazardous materials or following proper safety protocols in a BSL-3 laboratory. Therefore, the statement is false.Rate this question:
-
- 27.
How long do you wait until you can take the bucket out of the BSC and place it on the cart near the autoclave?
- A.
5 minutes
- B.
30 minutes
- C.
1 hour
- D.
There is no need to wait.
Correct Answer
B. 30 minutesExplanation
The correct answer is 30 minutes because it is a standard waiting time for the bucket to cool down after being in the BSC. This waiting period allows the bucket to reach a safe temperature before it can be handled and placed on the cart near the autoclave. Waiting for 30 minutes ensures that the bucket is cool enough to prevent any potential accidents or injuries.Rate this question:
-
- 28.
When replacing the aspiration jar, 400ml of what type of bleach should be added in the aspiration jar?
- A.
Undiluted bleach
- B.
Diluted bleach
Correct Answer
A. Undiluted bleachExplanation
When replacing the aspiration jar, it is necessary to add undiluted bleach. This means that the bleach should be used in its pure form, without any dilution or mixing with water. Adding undiluted bleach ensures that the concentration of bleach is strong enough to effectively disinfect and clean the aspiration jar. Diluted bleach, on the other hand, would have water mixed in, which would reduce its effectiveness in killing germs and bacteria. Therefore, undiluted bleach is the correct choice for maintaining proper sanitation in the aspiration jar.Rate this question:
-
- 29.
How long do you wait until you can take the nalgen aspiration jar out of the BSC and place it on the cart near the autoclave?
- A.
5 minutes
- B.
30 minutes
- C.
1 hour
- D.
2 hours
Correct Answer
B. 30 minutesExplanation
The correct answer is 30 minutes because it is important to wait for an adequate amount of time to allow any potential aerosols or contaminants to settle before removing the nalgen aspiration jar from the Biological Safety Cabinet (BSC). This waiting period helps to minimize the risk of exposure to harmful substances and ensures the safety of the individual handling the jar.Rate this question:
-
- 30.
Cite 3 common procedures that generate aerosols:
- A.
Vortexing
- B.
Centrifuging
- C.
Talking on the BSL-3 phone
- D.
Pipetting
- E.
Entering and exiting the BSL-3
Correct Answer(s)
A. Vortexing
B. Centrifuging
D. PipettingExplanation
The correct answer is vortexing, centrifuging, and pipetting. These three procedures are known to generate aerosols. Vortexing involves the rapid mixing of a sample using a vortex mixer, which can create aerosols by agitating the liquid. Centrifuging involves spinning samples at high speeds, causing the formation of aerosols as the liquid is forced out of the tubes. Pipetting, especially when done forcefully or with a large volume, can also generate aerosols by creating droplets in the air. These procedures are commonly performed in laboratories and require proper safety precautions to minimize the risk of aerosol exposure.Rate this question:
-
- 31.
When centrifuging infectious materials, each user must use the safety buckets for tubes with lids, or plate sealers and lids for plates.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
When centrifuging infectious materials, it is important for each user to use safety buckets for tubes with lids, or plate sealers and lids for plates. This is because centrifugation can cause the materials to become aerosolized, potentially spreading infectious particles into the surrounding environment. Using safety buckets or plate sealers with lids helps to contain and prevent the release of these particles, reducing the risk of exposure and contamination. Therefore, the statement "True" is correct.Rate this question:
-
- 32.
What are the only things that you should find in the BSC?
- A.
A bleach bucket, and pipette tips
- B.
A bleach bucket, a bleach squeeze bottle, and pipette tips
- C.
Pipette tips
- D.
A bleach bucket, an aspiration tray with 2 nalgen bottles and a bleach squeeze bottle, a reservoir, and pipette tips
Correct Answer
D. A bleach bucket, an aspiration tray with 2 nalgen bottles and a bleach squeeze bottle, a reservoir, and pipette tipsExplanation
The BSC (Biological Safety Cabinet) should contain a bleach bucket, an aspiration tray with 2 nalgen bottles and a bleach squeeze bottle, a reservoir, and pipette tips. These items are necessary for maintaining a clean and sterile working environment in the BSC. The bleach bucket is used for disinfecting surfaces, while the aspiration tray, nalgen bottles, and bleach squeeze bottle are used for containing and disposing of contaminated liquids. The reservoir is used for holding liquids during experiments, and pipette tips are essential for accurate and precise liquid handling.Rate this question:
-
- 33.
The solid waste container can remain open only when someone is working in the BSC.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The statement suggests that the solid waste container should only be open when someone is working in the Biological Safety Cabinet (BSC). This is likely because the BSC provides a controlled and safe environment for handling potentially hazardous materials, including solid waste. By keeping the container closed when not in use, it helps to minimize the risk of contamination or exposure to harmful substances. Therefore, the statement is true as it emphasizes the importance of maintaining safety protocols while working in the BSC.Rate this question:
-
- 34.
Give 3 reasons why the vacuum line should be turned off when leaving the BSC?
- A.
To avoid evaporation of the bleach
- B.
To avoid cluttering of the filter
- C.
To save energy
- D.
To reduce the noise
Correct Answer(s)
A. To avoid evaporation of the bleach
B. To avoid cluttering of the filter
C. To save energyExplanation
Turning off the vacuum line when leaving the BSC is important for several reasons. Firstly, it helps to avoid evaporation of the bleach, which can be harmful if inhaled or cause damage to nearby equipment. Secondly, it prevents cluttering of the filter by preventing unnecessary suction and accumulation of debris. Lastly, turning off the vacuum line helps to save energy by reducing power consumption when it is not needed.Rate this question:
-
- 35.
Why should you clean and leave the table on the side free of items?
- A.
To enable everyone to use the space as he/she wants.
- B.
Your samples will get contaminated otherwise.
- C.
To receive extra tickets in the BSL-3 users yearly raffle.
Correct Answer
A. To enable everyone to use the space as he/she wants.Explanation
Cleaning and leaving the table on the side free of items allows for a more flexible and accommodating space for everyone to use as they please. It ensures that individuals have the freedom to utilize the table for their own purposes without any restrictions or obstructions. This promotes inclusivity and allows for a better overall experience for all users of the space.Rate this question:
-
- 36.
What are the 4 pieces of information that must be written on all flasks placed in the incubator?
- A.
Initials
- B.
Media
- C.
Cell Type
- D.
Date
- E.
Lab
- F.
Infectious agent (if applicable drug resistance)
Correct Answer(s)
A. Initials
C. Cell Type
D. Date
F. Infectious agent (if applicable drug resistance)Explanation
The four pieces of information that must be written on all flasks placed in the incubator are initials, cell type, date, and infectious agent (if applicable drug resistance). These pieces of information are essential for proper identification and tracking of the flasks and their contents, ensuring accurate record-keeping and preventing any confusion or mix-ups in the incubator.Rate this question:
-
- 37.
The Deli fridge serves only for storing media and solution and should not be used to store any supernant/virus or other potentially infected suspension. What fridge should be used to store these supernatants?
- A.
Under the tissue centrifuge (BSL3 south wall)
- B.
In the deli Fridge anyway
- C.
In the media/elisa kit fridge
Correct Answer
A. Under the tissue centrifuge (BSL3 south wall)Explanation
The correct answer is "Under the tissue centrifuge (BSL3 south wall)". This is because the tissue centrifuge is located in a BSL3 (Biosafety Level 3) area, which is designed to handle potentially infectious materials. Storing supernatants, viruses, or other potentially infected suspensions in this area ensures proper containment and reduces the risk of contamination or spread of the infectious agents.Rate this question:
-
- 38.
It is the responsibility of the user to discard his/her unwanted culture and remove his/her culture from the incubator prior to the date of incubator cleaning (posted on the incubator)?
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The responsibility of the user is to discard their unwanted culture and remove their culture from the incubator before the scheduled cleaning. This implies that the user should not rely on the incubator cleaning to remove their culture, but should take proactive steps to remove it themselves. Therefore, the statement is true.Rate this question:
-
- 39.
What is the procedure to count cells with a Hemacytometer?
- A.
Prior to counting the cells, the user must add PFA in the trypan blue solution to reach 1% PFA final concentration.
- B.
Prior to counting the cells, the user must add PFA in the trypan blue solution to reach 0.1% PFA final concentration.
Correct Answer
A. Prior to counting the cells, the user must add PFA in the trypan blue solution to reach 1% PFA final concentration. -
- 40.
How long must samples be fixed in PFA 1% prior to removal from BSL3?
- A.
30 minutes
- B.
1 hour
- C.
Overnight
Correct Answer
B. 1 hourExplanation
Samples must be fixed in PFA 1% for 1 hour prior to removal from BSL3. Fixation is a process used to preserve the structure and integrity of biological samples for further analysis. In this case, PFA 1% is a commonly used fixative solution that contains 1% paraformaldehyde. By fixing the samples for 1 hour, any potential pathogens or hazardous materials present in the sample can be effectively neutralized, ensuring safe handling and transport outside of the BSL3 facility.Rate this question:
-
- 41.
How long should the incubation at >95˚C temperature be in order to inactivate the samples? How can the temperature be controlled?
- A.
1 minute trusting the heat block settings values
- B.
10 minutes trusting the heat block settings values
- C.
1 minute, verifying the temperature with a thermometer
- D.
10 minute, verifying the temperature with a thermometer
Correct Answer
D. 10 minute, verifying the temperature with a thermometerExplanation
The correct answer is 10 minutes, verifying the temperature with a thermometer. To ensure that the samples are properly inactivated, the incubation should be done for 10 minutes. It is important to verify the temperature with a thermometer to ensure that it is consistently maintained above 95˚C throughout the incubation period. Trusting the heat block settings values alone may not be sufficient as there could be variations or inaccuracies in the settings. Using a thermometer allows for accurate monitoring and control of the temperature.Rate this question:
-
- 42.
What do you do when the alarm of the freezers/liquid nitrogen room goes off?
- A.
Nothing
- B.
Exit the room and call facilities or BSL3 manager
Correct Answer
B. Exit the room and call facilities or BSL3 managerExplanation
When the alarm of the freezers/liquid nitrogen room goes off, the correct action is to exit the room immediately. This is because the alarm indicates that there may be a potential hazard or malfunction in the room, such as a temperature issue or a leak, which could be dangerous. After exiting, it is important to notify the appropriate personnel, such as facilities or the BSL3 manager, who can address the situation and ensure the safety of the area.Rate this question:
-
- 43.
Why should you store all your samples in boxes and a rack in the -80˚C freezer?
- A.
Because it looks nice
- B.
When there is a freezer failure, it is easier to transport the samples and retrieve the samples.
Correct Answer
B. When there is a freezer failure, it is easier to transport the samples and retrieve the samples.Explanation
Storing all samples in boxes and a rack in the -80˚C freezer is recommended because it makes it easier to transport and retrieve the samples in case of a freezer failure. This organization ensures that the samples are properly labeled and grouped together, making it more efficient to locate and handle them during emergencies. Additionally, the use of boxes and racks helps to maximize the storage space in the freezer, allowing for better organization and utilization of the available space.Rate this question:
-
- 44.
What do you do if the negative pressure alarm goes off?
- A.
Close your flasks and tubes
- B.
Call the BSL3 manager or facility
- C.
Exit the room
Correct Answer(s)
A. Close your flasks and tubes
B. Call the BSL3 manager or facility
C. Exit the roomExplanation
If the negative pressure alarm goes off, it indicates a potential issue with the containment system. Closing flasks and tubes helps prevent any potential release of hazardous materials. Calling the BSL3 manager or facility is important to report the alarm and seek guidance on the next steps. Exiting the room is necessary to ensure personal safety and minimize exposure to any potential hazards.Rate this question:
-
- 45.
To use the ultracentrifuge you are required to attend a special training.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The statement suggests that in order to use the ultracentrifuge, special training is necessary. This implies that there are specific skills or knowledge that individuals need to acquire before they can safely and effectively operate the ultracentrifuge. Therefore, the correct answer is true, indicating that attending special training is indeed required to use the ultracentrifuge.Rate this question:
-
- 46.
Where can you find the Needlestick hotline phone number?
- A.
On all phones of the BSL-3
- B.
In the BSL3 manual.
- C.
In the restroom
- D.
Posted on the wall of the BSL3 at the entrance and in the back of the room
- E.
Posted on the wall of the ante-room
Correct Answer(s)
A. On all phones of the BSL-3
B. In the BSL3 manual.
D. Posted on the wall of the BSL3 at the entrance and in the back of the room
E. Posted on the wall of the ante-roomExplanation
The correct answer is that you can find the Needlestick hotline phone number on all phones of the BSL-3, in the BSL3 manual, and posted on the wall of the BSL3 at the entrance and in the back of the room, as well as on the wall of the ante-room.Rate this question:
-
- 47.
Who is the only person that can train personnel and give them access to the BSL-3?
- A.
Susan Dzierson, Director of Human Resources
- B.
Mauricio Montano, BSL-3 Manager
- C.
Ruben Oregon, facilities engineer.
Correct Answer
B. Mauricio Montano, BSL-3 ManagerExplanation
The BSL-3 Manager, Mauricio Montano, is the only person who can train personnel and give them access to the BSL-3. This implies that he has the necessary expertise and authority to oversee the training and access procedures for personnel working in the BSL-3 facility. The other individuals mentioned, Susan Dzierson and Ruben Oregon, do not have the specific role or responsibility mentioned in the question.Rate this question:
-
- 48.
What do you do if you see someone that you don’t know in the BSL-3?
- A.
Introduce yourself.
- B.
They got in, so they must have BSL-3 privileges.
- C.
Ask them who they are and what they are doing, or call Mauricio Montano or someone from Facilities.
Correct Answer
C. Ask them who they are and what they are doing, or call Mauricio Montano or someone from Facilities.Explanation
In the BSL-3, it is important to maintain strict security measures and ensure that only authorized personnel have access. Therefore, if someone is seen in the BSL-3 whom you don't know, it is necessary to ask them who they are and what they are doing. This helps to verify their identity and purpose of being in the restricted area. If there are any doubts or concerns, it is advisable to contact Mauricio Montano or someone from the Facilities department who can further investigate the situation and ensure the safety and security of the facility.Rate this question:
-
- 49.
Samples can be taken out of the BSL-3 if they have been inactivated
- A.
By heat (10 minutes at 95˚C)
- B.
By PFA 0.1% final
- C.
By SDS or NP40 at 0.5% for 30 minutes.
- D.
By PFA at 1% for 1 hour.
Correct Answer(s)
A. By heat (10 minutes at 95˚C)
C. By SDS or NP40 at 0.5% for 30 minutes.
D. By PFA at 1% for 1 hour.Explanation
Samples can be taken out of the BSL-3 if they have been inactivated by heat (10 minutes at 95˚C), by SDS or NP40 at 0.5% for 30 minutes, and by PFA at 1% for 1 hour. These methods of inactivation ensure that any potential pathogens or infectious agents present in the samples are effectively neutralized, making them safe for handling and transport outside of the BSL-3 facility.Rate this question:
-
- 50.
Why should users make an effort to maintain the organization of each BSC and side shelf the same way?
- A.
Because otherwise your experiments won't work.
- B.
So it is easy for users to work and find things in any BSC.
- C.
Adds to the safe practices of the BSL3, and it makes it easy for users to work in any BSC.
- D.
The UCSF EH&S Department requires it.
Correct Answer
C. Adds to the safe practices of the BSL3, and it makes it easy for users to work in any BSC.Explanation
Maintaining the organization of each BSC and side shelf in the same way adds to the safe practices of the BSL3. This is because a consistent organization reduces the risk of cross-contamination and ensures that hazardous materials are properly stored. Additionally, it makes it easy for users to work in any BSC as they can quickly locate and access the necessary equipment and materials.Rate this question:
-
Back to top