Bsl-3 Quiz - Staff

50 Questions | Total Attempts: 106

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Bsl-3 Quiz - Staff - Quiz

All staff members using the BSL-3 must pass this quiz prior to working in the BSL-3.


Questions and Answers
  • 1. 
    If you are exposed to blood or OPIM that has been contaminated with HBV, your risk of acquiring serological evidence of an infection is:
    • Slightly less than_____%
  • 2. 
    If you are exposed to blood or OPIM that has been contaminated with HCV, your risk of acquiring serological evidence of an infection is:
    • ______%
  • 3. 
    If you are exposed to blood or OPIM that has been contaminated with HIV, your risk of acquiring serological evidence of an infection is:
    • Slightly less than ____%
  • 4. 
    What is an effective treatment/prevention method against HBV?
    • A. 

      Antibiotics

    • B. 

      HBV Vaccine

    • C. 

      Alcohol

  • 5. 
    The needlestick hotline (415) 353-7842 provides expedited access to post-exposure treatment, follow up and consulting.
    • A. 

      True

    • B. 

      False

  • 6. 
    When do you call the needlestick hotline?
    • A. 

      Immediately, because the effectiveness of the treatment is time sensitive.

    • B. 

      When you are done with your experiment.

    • C. 

      The next day after a sleepless night.

  • 7. 
    It is OK to give access to the BSL-3 to anyone who asks.
    • A. 

      True

    • B. 

      False

  • 8. 
    Minors can access the BSL-3 if accompanied by an adult.
    • A. 

      True

    • B. 

      False

  • 9. 
    Who is responsible for the safety of maintenance personnel or anyone else that you have given access to the BSL-3?
    • A. 

      The person who gives him/her access

    • B. 

      The BSL-3 Manager

    • C. 

      The Facilities Department

  • 10. 
    It is OK to use a cell phone or an iPod in the BSL3 if a user removes their outer gloves when holding it.
    • A. 

      True

    • B. 

      False

  • 11. 
    What is the personal protective equipment for the BSL3?
    • A. 

      Double gloves, gown and goggles

    • B. 

      Gloves and lab coat

  • 12. 
    To better notice potential defects or tears in outer gloves, the inside gloves should be of a darker color.
    • A. 

      True

    • B. 

      False

  • 13. 
    What are the two liquids used as decontaminants in the BSL-3?
    • A. 

      Bleach

    • B. 

      Soapy water

    • C. 

      Ethanol 70

    • D. 

      Antibiotic gel

  • 14. 
    Double decontamination of your wastes is recommended for highly infectious material. To do so, add bleach from the squirt bottle for a final concentration of 10%, decontaminate for 30 minutes, aspirate all liquids, discard in the appropriate dry waste container
    • A. 

      True

    • B. 

      False

  • 15. 
    Once the bleach bucket is filled with pipettes,
    • A. 

      Wait 30 minutes before draining the pipettes and discarding them in the solid waste containers

    • B. 

      Leave it aside in the BSC and bring a new bleach bucket filled half way with 10% bleach

  • 16. 
    Order the step of the to clean a biohazard spill a.     Add 10% bleach to soak the spill control pad and let it react for 30 minutes. b.     Place the spill control pad over liquid to absorb and control the spread of liquid. c.     Carefully remove pad, place into doubled autoclave bags. d.     Dispose of clean-up materials appropriately. e.    Decontaminate all exposed surfaces with 10% bleach disinfectant and rinse with 70% ethanol. f.     Remove personnel protective equipment (PPE), wash hands, and exit BSL-3 laboratory to the anteroom.
    • A. 

      A, b, c, d, e, and f

    • B. 

      B, a, c, d, e, and f

  • 17. 
    What do you do if you want to ship or transport non-inactivated samples out of the BSL3?
    • A. 

      Read the UCSF Shipment of Biological Materials Manual.

    • B. 

      Have a current bloodborne pathogen training certification from OEH&S.

    • C. 

      A current “Safe Shipping of Biohazards” training

    • D. 

      Have the approval of the BSL3 Lab Manager and the user's PI.

  • 18. 
    You need to get the approval from the BSL-3 Manager to bring new items into the BSL-3.
    • A. 

      True

    • B. 

      False

  • 19. 
    Is it OK to leave some liquid in the solid waste container? Why?
    • A. 

      No, it could leak on the floor. Then it is slippery.

    • B. 

      Yes, the plastic bags contain it.

    • C. 

      Yes, a small amount of liquid is not a danger.

  • 20. 
    The lid of the sharps containers must be clicked on it before use.
    • A. 

      True

    • B. 

      False

  • 21. 
    There are 5 waste containers around the BSC:
    1. bleach bucket
    2. aspiration jar
    3. small solid waste
    4. large solid waste
    5. sharps containers
    What items go into a bleach bucket?
    • A. 

      Emptied eppendorf tubes

    • B. 

      Tips

    • C. 

      Pipettes

    • D. 

      Liquid

    • E. 

      Recapped falcon tubes

    • F. 

      96v plates without liquid

    • G. 

      T175 previously treated for 30 minutes with 10% bleach

    • H. 

      Ultracentrifugation tubes

  • 22. 
    There are 5 waste containers around the BSC:
    1. bleach bucket
    2. aspiration jar
    3. small solid waste
    4. large solid waste
    5. sharps containers
    What items go into a sharps container?
    • A. 

      Emptied eppendorf tubes

    • B. 

      Tips

    • C. 

      96v plates without liquid

    • D. 

      Liquid

    • E. 

      Recapped falcon tubes

    • F. 

      Ultracentrifugation tubes that have no cap

    • G. 

      T175 previously treated for 30 minutes with 10% bleach

    • H. 

      Pipettes

  • 23. 
    There are 5 waste containers around the BSC:
    1. bleach bucket
    2. aspiration jar
    3. small solid waste
    4. large solid waste
    5. sharps containers
    What items go into a large solid waste container?
    • A. 

      Pipettes

    • B. 

      Recapped falcon tubes

    • C. 

      96v plates without liquid

    • D. 

      Ultracentrifugation tubes

    • E. 

      Tips

    • F. 

      Liquid

    • G. 

      Flasks previously treated for 30 minutes with 10% bleach

    • H. 

      Emptied eppendorf tubes

    • I. 

      Recapped Flasks

  • 24. 
    There are 5 waste containers around the BSC:
    1. bleach bucket
    2. aspiration jar
    3. small solid waste
    4. large solid waste
    5. sharps containers
    What items go into an aspiration jar?
    • A. 

      Ultracentrifugation tubes

    • B. 

      T175 previously treated for 30 minutes with 10% bleach

    • C. 

      96v plates without liquid

    • D. 

      Pipettes

    • E. 

      Tips

    • F. 

      Liquid

    • G. 

      Recapped falcon tubes

    • H. 

      Emptied eppendorf tubes

  • 25. 
    Removing the maximum amount of bleach from the waste being autoclaved avoids corrosion of the autoclave.
    • A. 

      True

    • B. 

      False

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