When inactivated with PFA for a final concentration of 1% PFA
When inactivated with PFA for a final concentration of 0.1% PFA
When inactivated by incubation for 10 min at 95°C
When lysed in 0.5 % SDS
When cells are pelleted and killed by freezing
When virus preparation are lysed for 10 min in 0.5% triton
One can enter the room if one is a trained BSL3 user
One should not enter the room when a sign is posted on the door
Yes, I will because I respect my colleagues and want to minimize their risk of exposure
No, I won’t because people should just wear close toe shoes in the BSL3
Yes
No
True
False
50%
0.5%
80%
15%
Read the UCSF Shipment of Biological Materials Manual
Possess a current ‘Bloodborn Pathogen’ training certificate from OEH&S
Have a current ‘Safe Shipping of Biohazards’ training
Have the BSL3 manager as well as ones PI approve the shipment
Nothing. One can ship or transport materials at anytime
Name of individual shipping the materials
Address of individual
Name and address of individual receiving the materials
Emergency contact name and number
Biohazard class
Name of PI
A happy face
Biohazard sticker
Inactivated samples
Activated samples
Supplies that are in sterile packaging and sprayed with ethanol
No materials or supplies can be taken out of the BSL3
Both A and C
Centrifuge
Vortex
Pipetting samples
Sneezing
Opening and closing doors
Anyone
All BSL3 users
Trained BSL3 users that have completed the ultracentrifuge training
Flow core staff
Emptied eppendorf tubes
Tips
96w plates without lid
Pipettes
Liquid
Recapped falcon tubes
T175 flasks
Ultracentrifuge tubes
Emptied eppendorf tubes
Tips
96w plates without lid
Pipettes
Liquid
Recapped falcon tubes
T175 flasks
Ultracentrifuge tubes without caps
Emptied eppendorf tubes
Tips
96w plates without lid
Pipettes
Liquid
Recapped falcon tubes
Recapped T175 flasks
Ultracentrifuge tubes without caps
Flasks previously treated with 10% bleach for 30 minutes
Emptied eppendorf tubes
Tips
96w plates without lid
Pipettes
Liquid
Recapped falcon tubes
Recapped T175 flasks
Ultracentrifuge tubes without caps
Flasks previously treated with 10% bleach for 30 minutes
Samples are easy to find
Metal racks keep the freezer organized so others can have space to store their samples
If labeled, others will not take ones sample
Samples will not get lost or thrown away
All of the above
Training and consulting
Equipment maintenance/repair
Daily decontamination of BSC
Disposal of solid biohazardous waste and bleach buckets
Disposal of sharp containers and vacuum jars
Ordering supplies
Writing supplies on board
Stocking materials
Bringing pipette tips in from the ante room
Training and consulting
Equipment maintenance/repair
Daily decontamination of BSC
Disposal of solid biohazardous waste and bleach buckets
Disposal of sharp containers and vacuum jars
Ordering supplies
Writing supplies on board
Stocking materials
Bringing pipette tips in from the ante room
Training and consulting
Equipment maintenance/repair
Daily decontamination of BSC
Disposal of solid biohazardous waste and bleach buckets
Disposal of sharp containers and vacuum jars
Ordering supplies
Writing supplies on board
Stocking materials
Bringing pipette tips in from the ante room
Training and consulting
Equipment maintenance/repair
Daily decontamination of BSC
Disposal of solid biohazardous waste and bleach buckets
Disposal of sharp containers and vacuum jars
Ordering supplies
Writing supplies on board
Stocking materials
Bringing pipette tips in from the ante room
0
2 (XL-90 & Beckman 6R)
No
Yes