2012 Bsl3 User Refresher Quiz

22 Questions | Total Attempts: 66

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2012 Bsl3 User Refresher Quiz

Questions and Answers
  • 1. 
    When can one take samples out of the BSL3?
    • A. 

      When inactivated with PFA for a final concentration of 1% PFA

    • B. 

      When inactivated with PFA for a final concentration of 0.1% PFA

    • C. 

      When inactivated by incubation for 10 min at 95°C

    • D. 

      When lysed in 0.5 % SDS

    • E. 

      When cells are pelleted and killed by freezing

    • F. 

      When virus preparation are lysed for 10 min in 0.5% triton

  • 2. 
    What should one do when a biohazardous sort is in progress?
    • A. 

      One can enter the room if one is a trained BSL3 user

    • B. 

      One should not enter the room when a sign is posted on the door

  • 3. 
    This year, on several occasions, a vial containing infectious virus (NL4-3) was found on the floor near the -80˚C freezer.  Will you make an effort and retrieve your vials if they fall on the floor?
    • A. 

      Yes, I will because I respect my colleagues and want to minimize their risk of exposure

    • B. 

      No, I won’t because people should just wear close toe shoes in the BSL3

  • 4. 
    Arrange the following steps in the correct order when handling a spill.  Do not use commas or spaces when answering (example - abcdef).
    1. Add 10% bleach to the absorbent material and let it sit for 30 minutes.
    2. Place absorbent material (paper towels, absorbent mats, etc) over the spill to control the spread of spill.
    3. Move to the ante room and remove personnel protective equipment (PPE), wash hands, and exit the BSL3.
    4. Carefully remove soiled absorbent material and place into doubled biohazard autoclave bags.
    5. Dispose the materials.
    6. Decontaminate all exposed surfaces with 10% bleach and rinse with 70% ethanol.
  • 5. 
    Is Herpes B possibly present in our BSL3?
    • A. 

      Yes

    • B. 

      No

  • 6. 
    A special training, including a Herpes B training, is required for users who handle non-human primate samples.
    • A. 

      True

    • B. 

      False

  • 7. 
    What is the approximate fatality rate if a human is infected with Herpes B?
    • A. 

      50%

    • B. 

      0.5%

    • C. 

      80%

    • D. 

      15%

  • 8. 
    What should be completed before one ships or transports biohazardous material out of the BSL3?
    • A. 

      Read the UCSF Shipment of Biological Materials Manual

    • B. 

      Possess a current ‘Bloodborn Pathogen’ training certificate from OEH&S

    • C. 

      Have a current ‘Safe Shipping of Biohazards’ training

    • D. 

      Have the BSL3 manager as well as ones PI approve the shipment

    • E. 

      Nothing. One can ship or transport materials at anytime

  • 9. 
    What needs to be written on the outside of the container when shipping biohazardous materials out of the BSL3?
    • A. 

      Name of individual shipping the materials

    • B. 

      Address of individual

    • C. 

      Name and address of individual receiving the materials

    • D. 

      Emergency contact name and number

    • E. 

      Biohazard class

    • F. 

      Name of PI

    • G. 

      A happy face

    • H. 

      Biohazard sticker

  • 10. 
    What materials or supplies can one take out of the BSL3?
    • A. 

      Inactivated samples

    • B. 

      Activated samples

    • C. 

      Supplies that are in sterile packaging and sprayed with ethanol

    • D. 

      No materials or supplies can be taken out of the BSL3

    • E. 

      Both A and C

  • 11. 
    What devices generate aerosols?
    • A. 

      Centrifuge

    • B. 

      Vortex

    • C. 

      Pipetting samples

    • D. 

      Sneezing

    • E. 

      Opening and closing doors

  • 12. 
    Who is allowed to use the ultracentrifuge?
    • A. 

      Anyone

    • B. 

      All BSL3 users

    • C. 

      Trained BSL3 users that have completed the ultracentrifuge training

    • D. 

      Flow core staff

  • 13. 
    What items go into the bleach bucket?
    • A. 

      Emptied eppendorf tubes

    • B. 

      Tips

    • C. 

      96w plates without lid

    • D. 

      Pipettes

    • E. 

      Liquid

    • F. 

      Recapped falcon tubes

    • G. 

      T175 flasks

    • H. 

      Ultracentrifuge tubes

  • 14. 
    What items go into the sharps container?
    • A. 

      Emptied eppendorf tubes

    • B. 

      Tips

    • C. 

      96w plates without lid

    • D. 

      Pipettes

    • E. 

      Liquid

    • F. 

      Recapped falcon tubes

    • G. 

      T175 flasks

    • H. 

      Ultracentrifuge tubes without caps

  • 15. 
    What items go into the large solid waste container?
    • A. 

      Emptied eppendorf tubes

    • B. 

      Tips

    • C. 

      96w plates without lid

    • D. 

      Pipettes

    • E. 

      Liquid

    • F. 

      Recapped falcon tubes

    • G. 

      Recapped T175 flasks

    • H. 

      Ultracentrifuge tubes without caps

    • I. 

      Flasks previously treated with 10% bleach for 30 minutes

  • 16. 
    What items go into the aspiration jar?
    • A. 

      Emptied eppendorf tubes

    • B. 

      Tips

    • C. 

      96w plates without lid

    • D. 

      Pipettes

    • E. 

      Liquid

    • F. 

      Recapped falcon tubes

    • G. 

      Recapped T175 flasks

    • H. 

      Ultracentrifuge tubes without caps

    • I. 

      Flasks previously treated with 10% bleach for 30 minutes

  • 17. 
    Why should one label and organize their samples into boxes and metal racks in the -80°C and -30°C freezers?
    • A. 

      Samples are easy to find

    • B. 

      Metal racks keep the freezer organized so others can have space to store their samples

    • C. 

      If labeled, others will not take ones sample

    • D. 

      Samples will not get lost or thrown away

    • E. 

      All of the above

  • 18. 
    Marielle, the BSL3 Director, is responsible for which of the following tasks:
    • A. 

      Training and consulting

    • B. 

      Equipment maintenance/repair

    • C. 

      Daily decontamination of BSC

    • D. 

      Disposal of solid biohazardous waste and bleach buckets

    • E. 

      Disposal of sharp containers and vacuum jars

    • F. 

      Ordering supplies

    • G. 

      Writing supplies on board

    • H. 

      Stocking materials

    • I. 

      Bringing pipette tips in from the ante room

  • 19. 
    Jaime, the BSL3 Manager, is responsible for which of the following tasks:
    • A. 

      Training and consulting

    • B. 

      Equipment maintenance/repair

    • C. 

      Daily decontamination of BSC

    • D. 

      Disposal of solid biohazardous waste and bleach buckets

    • E. 

      Disposal of sharp containers and vacuum jars

    • F. 

      Ordering supplies

    • G. 

      Writing supplies on board

    • H. 

      Stocking materials

    • I. 

      Bringing pipette tips in from the ante room

  • 20. 
    Nick, the Lab Aide, is responsible for which of the following tasks:
    • A. 

      Training and consulting

    • B. 

      Equipment maintenance/repair

    • C. 

      Daily decontamination of BSC

    • D. 

      Disposal of solid biohazardous waste and bleach buckets

    • E. 

      Disposal of sharp containers and vacuum jars

    • F. 

      Ordering supplies

    • G. 

      Writing supplies on board

    • H. 

      Stocking materials

    • I. 

      Bringing pipette tips in from the ante room

  • 21. 
    The BSL3 User is responsible for which of the following tasks:
    • A. 

      Training and consulting

    • B. 

      Equipment maintenance/repair

    • C. 

      Daily decontamination of BSC

    • D. 

      Disposal of solid biohazardous waste and bleach buckets

    • E. 

      Disposal of sharp containers and vacuum jars

    • F. 

      Ordering supplies

    • G. 

      Writing supplies on board

    • H. 

      Stocking materials

    • I. 

      Bringing pipette tips in from the ante room

  • 22. 
    How many ultracentrifuges are in the BSL3?  Is training required for each ultracentrifuge?
    • A. 

      0

    • B. 

      2 (XL-90 & Beckman 6R)

    • C. 

      No

    • D. 

      Yes

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