2012 Bsl3 User Refresher Quiz
- 1.When can one take samples out of the BSL3?
- A.
When inactivated with PFA for a final concentration of 1% PFA
- B.
When inactivated with PFA for a final concentration of 0.1% PFA
- C.
When inactivated by incubation for 10 min at 95°C
- D.
When lysed in 0.5 % SDS
- E.
When cells are pelleted and killed by freezing
- F.
When virus preparation are lysed for 10 min in 0.5% triton
- 2.What should one do when a biohazardous sort is in progress?
- A.
One can enter the room if one is a trained BSL3 user
- B.
One should not enter the room when a sign is posted on the door
- 3.This year, on several occasions, a vial containing infectious virus (NL4-3) was found on the floor near the -80˚C freezer. Will you make an effort and retrieve your vials if they fall on the floor?
- A.
Yes, I will because I respect my colleagues and want to minimize their risk of exposure
- B.
No, I won’t because people should just wear close toe shoes in the BSL3
- 4.Arrange the following steps in the correct order when handling a spill. Do not use commas or spaces when answering (example - abcdef).
- Add 10% bleach to the absorbent material and let it sit for 30 minutes.
- Place absorbent material (paper towels, absorbent mats, etc) over the spill to control the spread of spill.
- Move to the ante room and remove personnel protective equipment (PPE), wash hands, and exit the BSL3.
- Carefully remove soiled absorbent material and place into doubled biohazard autoclave bags.
- Dispose the materials.
- Decontaminate all exposed surfaces with 10% bleach and rinse with 70% ethanol.
- 5.Is Herpes B possibly present in our BSL3?
- A.
Yes
- B.
No
- 6.A special training, including a Herpes B training, is required for users who handle non-human primate samples.
- A.
True
- B.
False
- 7.What is the approximate fatality rate if a human is infected with Herpes B?
- A.
50%
- B.
0.5%
- C.
80%
- D.
15%
- 8.What should be completed before one ships or transports biohazardous material out of the BSL3?
- A.
Read the UCSF Shipment of Biological Materials Manual
- B.
Possess a current ‘Bloodborn Pathogen’ training certificate from OEH&S
- C.
Have a current ‘Safe Shipping of Biohazards’ training
- D.
Have the BSL3 manager as well as ones PI approve the shipment
- E.
Nothing. One can ship or transport materials at anytime
- 9.What needs to be written on the outside of the container when shipping biohazardous materials out of the BSL3?
- A.
Name of individual shipping the materials
- B.
Address of individual
- C.
Name and address of individual receiving the materials
- D.
Emergency contact name and number
- E.
Biohazard class
- F.
Name of PI
- G.
A happy face
- H.
Biohazard sticker
- 10.What materials or supplies can one take out of the BSL3?
- A.
Inactivated samples
- B.
Activated samples
- C.
Supplies that are in sterile packaging and sprayed with ethanol
- D.
No materials or supplies can be taken out of the BSL3
- E.
Both A and C
- 11.What devices generate aerosols?
- A.
Centrifuge
- B.
Vortex
- C.
Pipetting samples
- D.
Sneezing
- E.
Opening and closing doors
- 12.Who is allowed to use the ultracentrifuge?
- A.
Anyone
- B.
All BSL3 users
- C.
Trained BSL3 users that have completed the ultracentrifuge training
- D.
Flow core staff
- 13.What items go into the bleach bucket?
- A.
Emptied eppendorf tubes
- B.
Tips
- C.
96w plates without lid
- D.
Pipettes
- E.
Liquid
- F.
Recapped falcon tubes
- G.
T175 flasks
- H.
Ultracentrifuge tubes
- 14.What items go into the sharps container?
- A.
Emptied eppendorf tubes
- B.
Tips
- C.
96w plates without lid
- D.
Pipettes
- E.
Liquid
- F.
Recapped falcon tubes
- G.
T175 flasks
- H.
Ultracentrifuge tubes without caps
- 15.What items go into the large solid waste container?
- A.
Emptied eppendorf tubes
- B.
Tips
- C.
96w plates without lid
- D.
Pipettes
- E.
Liquid
- F.
Recapped falcon tubes
- G.
Recapped T175 flasks
- H.
Ultracentrifuge tubes without caps
- I.
Flasks previously treated with 10% bleach for 30 minutes
- 16.What items go into the aspiration jar?
- A.
Emptied eppendorf tubes
- B.
Tips
- C.
96w plates without lid
- D.
Pipettes
- E.
Liquid
- F.
Recapped falcon tubes
- G.
Recapped T175 flasks
- H.
Ultracentrifuge tubes without caps
- I.
Flasks previously treated with 10% bleach for 30 minutes
- 17.Why should one label and organize their samples into boxes and metal racks in the -80°C and -30°C freezers?
- A.
Samples are easy to find
- B.
Metal racks keep the freezer organized so others can have space to store their samples
- C.
If labeled, others will not take ones sample
- D.
Samples will not get lost or thrown away
- E.
All of the above
- 18.Marielle, the BSL3 Director, is responsible for which of the following tasks:
- A.
Training and consulting
- B.
Equipment maintenance/repair
- C.
Daily decontamination of BSC
- D.
Disposal of solid biohazardous waste and bleach buckets
- E.
Disposal of sharp containers and vacuum jars
- F.
Ordering supplies
- G.
Writing supplies on board
- H.
Stocking materials
- I.
Bringing pipette tips in from the ante room
- 19.Jaime, the BSL3 Manager, is responsible for which of the following tasks:
- A.
Training and consulting
- B.
Equipment maintenance/repair
- C.
Daily decontamination of BSC
- D.
Disposal of solid biohazardous waste and bleach buckets
- E.
Disposal of sharp containers and vacuum jars
- F.
Ordering supplies
- G.
Writing supplies on board
- H.
Stocking materials
- I.
Bringing pipette tips in from the ante room
- 20.Nick, the Lab Aide, is responsible for which of the following tasks:
- A.
Training and consulting
- B.
Equipment maintenance/repair
- C.
Daily decontamination of BSC
- D.
Disposal of solid biohazardous waste and bleach buckets
- E.
Disposal of sharp containers and vacuum jars
- F.
Ordering supplies
- G.
Writing supplies on board
- H.
Stocking materials
- I.
Bringing pipette tips in from the ante room
- 21.The BSL3 User is responsible for which of the following tasks:
- A.
Training and consulting
- B.
Equipment maintenance/repair
- C.
Daily decontamination of BSC
- D.
Disposal of solid biohazardous waste and bleach buckets
- E.
Disposal of sharp containers and vacuum jars
- F.
Ordering supplies
- G.
Writing supplies on board
- H.
Stocking materials
- I.
Bringing pipette tips in from the ante room
- 22.How many ultracentrifuges are in the BSL3? Is training required for each ultracentrifuge?
- A.
0
- B.
2 (XL-90 & Beckman 6R)
- C.
No
- D.
Yes
Questions: 15 | Attempts: 104 | Last updated: May 1, 2015
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