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Which of the following strategies would be most effective in achieving this? An experiment was designed to obtained nonspecific transcription from both strands of a DNA molecule.



A. Include the RNA holoenzyme in the reaction.
B. Use the core enzyme of RNA polymerase
C. Enrich the preparation with sigma subunit
D. Use intact DNA.
E. Include the RNA holoenzyme in the reaction and use the core enzyme of RNA polymerase are both effective

This question is part of Molecular Biology Exam 2
Asked by Ekanye, Last updated: Feb 12, 2020

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John Smith

John Smith

Answered Mar 16, 2017

Use the core enzyme of RNA polymerase
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NABEELAMAJID

NABEELAMAJID

Replied on May 13, 2017

I think its option e bcoz for transcription to star sigma factor is needed for recognition of promoter sequence and that is the component of holoenzyme.

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