The correct answer to this question is D, Increase the temperature by 10 C. At first, when a substrate concentration increases, so will the rate of the enzyme-catalyzed reaction, when substrate saturates the enzyme molecules, an increase in the reaction rate decreases. Enzyme-catalyzed reactions are known to increase when the enzyme concentration increases.
A low temperature, like 10 C, will increase the rate of the response. The high temperature would decrease the frequency of reaction, which will also limit its activity. Many activities can be done to understand this concept, and the substrate must be present at a high amount for it to be effective.
Increasing the temperature will make the molecules move faster, thus increasing the amount of collisions between substrate and enzyme. all the other three decrease rate: reducing the amount of substrate or enzyme reduces chances of collison, while changing the pH more than 1 number will most likely cause the enzyme to denature.