Which of the following hybridoma production steps is NOT correct? The aim of monoclonal antibody production is to produce cells that only secrete immunoglobulin directed against the antigen used in immunization.
A. Immunize a mouse with the antigen of choice then remove the spleen when the mouse is making an antibody response. B. Fuse the immune spleen cells with a myeloma tumor cell C. The cells are cultured in a selective medium allowing fused and non-fused cells to survive. D. Cells are grown in individual culture plate wells, and culture supernatants from wells contain growing hybrid cells are screened for the presence of the desired antibody by an enzyme-linked immunosorbent assay (ELISA) E. This clone (hybridoma) is an immortal producer of the desired monoclonalantibody