Why won't this experiment work? - ProProfs Discuss
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Why won't this experiment work?

For a science fair project, two students decided to repeat the  Hershey and Chase experiment, with modifications. They decided to label the nitrogen of the DNA, rather than the phosphate. They reasoned that each nucleotide has only one phosphate and two to five nitrogens. Thus, labeling the nitrogens would provide a stronger signal than labeling the phosphates.

Asked by Muitran, Last updated: Mar 03, 2024

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2 Answers

Matz Lewis Clark

Matz Lewis Clark

Traveler and writer by profession.

Matz Lewis Clark
Matz Lewis Clark, College student, Graduation, Orlando

Answered Feb 28, 2019

It should be noted that amino acids are also equipped with nitrogen atoms which means that it also contains proteins. The main goal of the experiment is to distinguish between the DNA and proteins but this is not possible if both of them have it.

The radioactivity will not be useful in checking what’s wrong. It is best if the two students would think of another project that may produce more productive results. It will also be advisable if they would also think of making other changes or modifications to the experiment that they want to re-do.

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John Smith

John Smith

John Smith
John Smith

Answered Dec 19, 2016

Amino acids (and thus proteins) also have nitrogen atoms; thus, the radioactivity would not distinguish between DNA and proteins.
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