PBS and HBSS are two forms of saline solution. PBS is the short form of Phosphate buffered saline, while HBSS is the short form of Hank’s balanced salt solution. Most times, people tend to confuse the two simply because they are both used as a solution when it comes to biological research and experiments.
Both solutions are good for biological experiments because they always strive to ensure a neutral solution. The first difference between the two solutions is that they are made with different ingredients.
Ingredients such as sodium phosphate, sodium chloride, and water are used in making Phosphate buffered saline (PBS), while materials such as magnesium, potassium, calcium, sodium, and chloride, are used in making Hank’s balanced salt solution. Hank’s balanced salt solution can be made to look like a powder, and it can also come in liquid form. On the other hand, PBS comes only in liquid form, and it is easier to make compared to HBSS.