What is the concentration of protein in your milk sample if your unknown milk sample has an absorbance of 0.73 at 595nm? You measure the absorbance values of five standard solutions of protein and you plot absorbance vs. concentration (see graph) and generate a linear trend line for your data.
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Answered Jan 25, 2019
The answer to this is 5.93mM. It can sometimes be challenging to compute for this when you do not know the proper method to use. This is always the first step. Select the best method that you think will be most effective in computing for the protein.
Some of the possible methods are the Bradford Method or the Lowry Method. Another method that you can use is to compare it to a standard curve which means that you are going to create your own method for protein concentration. Once you have already figured out the best method, the computation will not be hard do anymore.