What type of PCR quantifies the rate at which new DNA is synthesized? - ProProfs Discuss
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What type of PCR quantifies the rate at which new DNA is synthesized?





A. Real Time
B. Allele Specific
C. Multiplex
D. Qualitative

This question is part of PCR quiz
Asked by Drcoetzer, Last updated: May 23, 2020

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2 Answers

L. Cooper

Analyst by profession but writer by heart.

L. Cooper, Data Analyst, MCA, Newcastle

Answered Nov 01, 2019

The correct answer to this question is option A – Real time. Just as the name implies, real-time PCR is the type of PCR that quantifies the rate at which new DNA is synthesized. Real-time PCR monitors the progress by using a florescent dyes reporter molecule. The fluorescent emitted by the reporter allows early detection of PCR production early in the reaction.

Compare to the traditional PCR; real-time PCR is less time consuming due to the ability to detect amplification early in each cycle. Real-time PCR does not require post PCR processing. Real-time PCR can be used in all applications, just like the traditional PCR mostly in biological science, genetic expression analysis, and genetic testing.

 

drcoetzer

Drcoetzer

Answered Mar 14, 2018

Real Time

Real-time polymerase chain reaction is a laboratory technique based on the PCR, which is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification of one or more specific sequences in a DNA sample.
 

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