Which of the following is not true about Taq DNA polymerase?
A. It functions at extremely high temperatures B. It adds nucleotide triphosphates to form a new DNA strand C. It requires a salt environment (buffer) to work D. It can Act as an exonuclease to cut single-stranded DNA
The correct answer to this question is option D – It can ACT as an exonuclease to cut single stranded DNA Taq DNA polymerase also as Taq pol is a DNA polymerase commonly used in PCR to amplify segments of DNA. The optimal level of activity of Taq pol is seen at approximately 72 degrees Celsius.
This means it functions at extremely high temperature (option A). It functions to add nucleotide triphosphates to form a new DNA strand (option B). For Taq pol to work, PCR must be done in a buffer (salt) environment. This help create a suitatable chemical environment for Taq pol to function (option C) Taq pol does not have 3’ to 5’ exonuclease activity. It only exhibits 5’ exonuclease activity.
It can act as an exonuclease to cut single-stranded DNA
Taq polymerase It lacks a 3 to 5 exonuclease proofreading activity. Taq polymerase is a thermostable DNA polymerase. It is often abbreviated to Taq Pol (or simply Taq), and is frequently used in polymerase chain reaction (PCR), methods for greatly amplifying short segments of DNA.