What is a hot start? - ProProfs Discuss
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What is a hot start?





A. When all primers anneal to the open DNA template simultaneously
B. When a high temperature is maintained before polymerase is added (reduces non-specific primer binding)
C. When primers are added after the polymerase
D. When the starting temperature is between 110-120 degrees Celsius

This question is part of PCR quiz
Asked by Drcoetzer, Last updated: Nov 01, 2019

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2 Answers

A. Lucius

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A. LuciusSenior Content writer, Diploma in Literature, Dover, Delaware
Senior Content writer, Diploma in Literature, Dover, Delaware

Answered on Nov 01, 2019

The correct answer to the question is option B – When a high temperature is maintained before polymerase is added (reduces nonspecific primer binding). A hot start is a form of modification of traditional Polymerase Chain Reaction (PCR). It is one of the most advanced modifications of PCR. In the hot start, DNA polymerase is inactivated at low temperatures, which avoid nonspecific amplification of DNA.

Nonspecific binding was a major cause of concern in PCR because of the possible mispriced of a false prime target. This caused an increase in false results. Hot start prevents the nonspecific binding step, and this leads to a reduction information of primer dimmer and nonspecific priming. At the end of it all, the hot start will lead to better and increase product yield.

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drcoetzer

Drcoetzer

Answered on Nov 20, 2017

When a high temperature is maintained before polymerase is added (reduces non-specific primer binding)

Hot-start PCR: a technique that reduces non-specific amplification during the initial set up stages of the PCR.
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