What does polymerase chain reaction(PCR) do? - ProProfs Discuss
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What does polymerase chain reaction(PCR) do?



A. Makes it possible to create huge numbers of copies of tiny pieces of DNA
B. Utilizes RNA messenger RNA molecules from small pieces of DNA
C. Can create messenger RNA molecules from small pieces of DNA
D. Enables researchers to determine the sequence of a complementary strand of DNA when they have only single-stranded DNA

This question is part of Chapter 5 DNA, Gene, Expression, and biotechnology
Asked by Alishacashwell, Last updated: Nov 01, 2019

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2 Answers

A. Lucius

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A. LuciusSenior Content writer, Diploma in Literature, Dover, Delaware
Senior Content writer, Diploma in Literature, Dover, Delaware

Answered on Nov 01, 2019

The correct answer is option A – Make it possible to create huge numbers of copies of tiny pieces of DNA. Polymerase Chain Reaction (PCR) is the most common lab technique that we use to amplify DNA. We use this technique mostly in molecular biology for creating multiple copies of DNA from a sample. The process of PCR occurs in 3 phases . The denaturation phase, annealing phase and the extension (elongation) phase. The first phase is the denaturation phase. In this phase; temperature is raised to 92 degree Celsius.

The heat helps breakdown hydrogen bonds in the strands. In the annealing phase, the temperature is lowered to about 50 to 60 degree Celsius. This will allow primers to attach to specific regions on the DNA through hydrogen bonding. The elongation step is the final step in polymerase chain reaction. The temperature is raised again to allow the synthesis of new DNA strands.

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alishacashwell

Alishacashwell

Answered on Nov 19, 2017

Makes it possible to create huge numbers of copies of tiny pieces of DNA
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