Answer updated on: 05 Jun 2019
Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
Answer updated on: 15 Nov 2018
Kary received a Nobel Prize in chemistry in 1993, for his invention of the polymerase chain reaction (PCR). The process, which Kary Mullis conceptualized in 1983, is hailed as one of the monumental scientific techniques of the twentieth century.
Answer updated on: 09 Sep 2018
Typically, PCR consists of a series of 20-40 repeated temperature changes. This is called expontential amplification because each PCR cycle doubles the number of target DNA products.
Answer updated on: 23 Apr 2018
The original polynucleotide strand of DNA or RNA serves as a template to guide the synthesis of the new complementary polynucleotide of DNA or RNA.
Answer updated on: 14 Mar 2018
Real-time polymerase chain reaction is a laboratory technique based on the PCR, which is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification of one or more specific sequences in a DNA sample.
Answer updated on: 05 Mar 2018
Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 7580 °C, and commonly a temperature of 72 °C is used with this enzyme. At this step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTPs that are complementary to the ...
Answer updated on: 14 Feb 2018
Primers bind to the newly separated DNA strand
Lowering the temperature allows primers to anneal to single standed DNA. Stable DNA-DNA hydrogen bonds are only formed when the primer sequence very closely matches the template sequence.
Answer updated on: 05 Feb 2018
It can act as an exonuclease to cut single-stranded DNA
Taq polymerase It lacks a 3 to 5 exonuclease proofreading activity. Taq polymerase is a thermostable DNA polymerase. It is often abbreviated to Taq Pol (or simply Taq), and is frequently used in polymerase chain reaction (PCR), methods for greatly amplifying short segments of DNA.
Answer updated on: 20 Nov 2017
When a high temperature is maintained before polymerase is added (reduces non-specific primer binding)
Hot-start PCR: a technique that reduces non-specific amplification during the initial set up stages of the PCR.
Answer updated on: 19 Nov 2017
Denature DNA, Anneal Primers, Extend DNA
PCR relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and ...
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