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Answer updated on: 24 Feb 2020
Extension/elongation
Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 7580 C, and commonly a temperature of 72 C is used with this enzyme. At this step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTPs that are complementary to the template ...
Answer updated on: 12 Feb 2020
It can act as an exonuclease to cut single-stranded DNATaq polymerase It lacks a 3 to 5 exonuclease proofreading activity. Taq polymerase is a thermostable DNA polymerase. It is often abbreviated to Taq Pol (or simply Taq), and is frequently used in polymerase chain reaction (PCR), methods for greatly amplifying short segments of DNA.
Answer updated on: 12 Feb 2020
Real TimeReal-time polymerase chain reaction is a laboratory technique based on the PCR, which is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification of one or more specific sequences in a DNA sample.
Answer updated on: 12 Feb 2020
Denature DNA, Anneal Primers, Extend DNAPCR relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated ...
Answer updated on: 12 Feb 2020
Polymerase Chain ReactionThe polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
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