Quiz Online 6 Chapter 7 The Polymerase Chain Reaction

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Quiz Online 6 Chapter 7 The Polymerase Chain Reaction
Belajar tanpa berpikir tidak ada gunanya, sedangkan berpikir tanpa belajar adalah berbahaya. Mohon diperhatikan untuk perkuliahan besok (9 Juni 2009). Setiap mahasiswa diharuskan menyiapkan pertanyaan terkait topik kuliah PCR. Pertanyaan harus merujuk pada materi kuliah Chapter 7 dengan menyebutkan nomor halaman sesuai dengan pertanyaan yang diajukan. Jika pertanyaan tidak sesuai dengan materi kuliah maka nilai kuis akan dikurang satu nomor.

  
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  • 1. 
    The product of a polymerase chain reaction.
    • A. 

      Amplicon

    • B. 

      Oligonucleotide

    • C. 

      Primer extension

    • D. 

      DNA templates

    • E. 

      Thermal cycler


  • 2. 
    Synthesis of a copy of a nucleic acid from a primer.
    • A. 

      Primer extension

    • B. 

      Amplicon

    • C. 

      Oligonucleotide

    • D. 

      DNA templates

    • E. 

      Thermal cycler


  • 3. 
    Heating/cooling system for PCR applications. Enables denaturation, primer binding, and extension cycles to be programmed and automated.
    • A. 

      Primer extension

    • B. 

      Amplicon

    • C. 

      Oligonucleotide

    • D. 

      DNA templates

    • E. 

      Thermal cycler


  • 4. 
    Thermostable DNA polymerases such as Taq polymerase have enabled automation of the PCR process without the need for adding fresh polymerase after the denaturation stage of each cycle.
    • A. 

      TRUE

    • B. 

      FALSE


  • 5. 
    The key requirements for the PCR are a DNA template, a pair of suitable oligonucleotide primers, and a DNA polymerase.
    • A. 

      TRUE

    • B. 

      FALSE


  • 6. 
    The length of the target sequence is defined by the 5’ ends of the primers.
    • A. 

      TRUE

    • B. 

      FALSE


  • 7. 
    The primers should flank the target sequence.
    • A. 

      TRUE

    • B. 

      FALSE


  • 8. 
    In PCR, DNA polymerase is purified from the thermophilic bacterium Thermus aquaticus which inhabits hot springs.
    • A. 

      TRUE

    • B. 

      FALSE


  • 9. 
    Duplex DNA is heat denatured to give single strands, and two oligonucleotide primers are annealed to their complementary sequences on the target DNA.
    • A. 

      TRUE

    • B. 

      FALSE


  • 10. 
    Nested PCR involves using two sets of primers.
    • A. 

      TRUE

    • B. 

      FALSE


  • 11. 
    RAPD-PCR based on lowstringency annealing of primers.
    • A. 

      TRUE

    • B. 

      FALSE


  • 12. 
    Once the PCR process has been completed, the DNA fragments that have been amplified can be analysed by using agarose gel electrophoresis.
    • A. 

      TRUE

    • B. 

      FALSE


  • 13. 
    The PCR can be used to clone specific sequences.
    • A. 

      TRUE

    • B. 

      FALSE


  • 14. 
    The PCR can be used to clone genes from one organism by using priming sequences from another if some sequence data are available for the gene.
    • A. 

      TRUE

    • B. 

      FALSE


  • 15. 
    Amplified fragment length (AFLP) polymorphism Genome analysis by PCR of restriction digests of genomic DNA.
    • A. 

      TRUE

    • B. 

      FALSE


  • 16. 
    One use of RT-PCR is in determining the amount of mRNA in a sample.
    • A. 

      TRUE

    • B. 

      FALSE


  • 17. 
    A typical temperature profile of PCR steps would be denaturation at 95◦C, primer annealing at 55◦C, and extension by Taq polymerase at 72◦C.
    • A. 

      TRUE

    • B. 

      FALSE


  • 18. 
    Polymerase chain reaction (PCR) is a method for the selective amplification of DNA sequences.
    • A. 

      TRUE

    • B. 

      FALSE


  • 19. 
    Random amplified polymorphic DNA (RAPD) is PCR-based method of DNA profiling that involved amplification of sequences using random primers.
    • A. 

      TRUE

    • B. 

      FALSE


  • 20. 
    RT-PCR Reverse transcriptase (transcription) is PCR, where a  cDNA copy of mRNA is made and then amplified using PCR.
    • A. 

      TRUE

    • B. 

      FALSE


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