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Molecular Biology Exam #3

50 Questions  I  By Ekanye
Molecular Biology Quizzes & Trivia
Third Exam!

  
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Question Excerpt

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1.  Please put the following steps of RNA Cap synthesis in the correct order. (1) N7 of the capping guanine is methylated(2) The terminal phosphate is removed from the pre-mRNA.(3) A capping GMP is added to the pre-mRNA(4) the 2'-O-methyl group of the penultimate nucleotide is methylated 
A.
B.
C.
D.
E.
2.  When does capping of the pre-mRNA occur? 
A.
B.
C.
D.
E.
3.  You have isolated a new species of mRNA using an oligo dT column. You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns. Below is a representation of the electron micrograph results. Which of the following can you conclude from your experiment? 
A.
B.
C.
D.
E.
4.  Which of the following experiments was useful in determining the existence of introns in the adenovirus genome? 
A.
B.
C.
D.
E.
5.  The first two bases and the last two bases in the splicing signal consensus sequences are 
A.
B.
C.
D.
E.
6.  Which of the following is the name for the yeast 40S particle where mRNA splicing takes place? 
A.
B.
C.
D.
E.
7.  "Snurps" or snRNPs are composed of 
A.
B.
C.
D.
E.
8.  Bioinformatics databases include all EXCEPT 
A.
B.
C.
D.
E.
9.  DNA microarrays can be used to study
A.
B.
C.
D.
E.
10.  Genomic sequence information can be applied to study which of the following? 
A.
B.
C.
D.
E.
11.  The catalytic center of the spliceosome appears to include 
A.
B.
C.
D.
E.
12.  Which of the following snRNP is mismatched with its function? 
A.
B.
C.
D.
E.
13.  Spliceosomal lariat is 
A.
B.
C.
D.
E.
14.  Which of the followign is NOT a function of the mRNA Cap? 
A.
B.
C.
D.
E.
15.  Although snRNA U1 functions in the nucleus, it receives a 5' Cap to enhance its 
A.
B.
C.
D.
E.
16.  Which of the following is the mRNA polyadenylation signal motif found in mammalian cells? 
A.
B.
C.
D.
E.
17.  Which of the following was the first free living organism to have its genome sequenced? 
A.
B.
C.
D.
E.
18.  Which of the following free living organisms have the smallest genome, containing about only 470 genes? 
A.
B.
C.
D.
E.
19.  Which is not correct about annotated genes? 
A.
B.
C.
D.
E.
20.  Which of the following is not required for mammalian cell polyadenylation of pre-mRNA? 
A.
B.
C.
D.
E.
21.  The Shine-Dalgarno sequence can be found in 
A.
B.
C.
D.
E.
22.  Which of the following is not a finding from analysis of the complete DNA sequence of human chromosome 22? 
A.
B.
C.
D.
E.
23.  The estimated total number of genes in the human genome is 
A.
B.
C.
D.
E.
24.  The shine-Dalgarno sequence is 
A.
B.
C.
D.
E.
25.  Put the followign steps of prokaryotic translation initiation in the correct order (1) Binding of IF1, IF2, and GTP to the 30s subunit (2) Binding of IF3 to the 30S subunit (3) Binding of the 50S subunit and loss of IF1 and IF3(4) Dissociation of the 70S ribosome (5) Formation of the 70S initiation complex by dissociation of IF2 and GTP hydrolysis (6) formation of the 30S initiation complex 
A.
B.
C.
D.
E.
26.  Which of the following statements is not true concerning the genetic code? 
A.
B.
C.
D.
E.
27.  Genetic code has all EXCEPT 
A.
B.
C.
D.
E.
28.  About direction of translation, which is likely? 
A.
B.
C.
D.
E.
29.  Which of the following initiation factors prevents re-association of the 70s ribosome by binding to the free 30s subunit? 
A.
B.
C.
D.
E.
30.  Which of the following is not part of the 30S initiation complex? 
A.
B.
C.
D.
E.
31.  Which of the following is the best definition of translation? 
A.
B.
C.
D.
E.
32.  Which if false? 
A.
B.
C.
D.
E.
33.  Function of EF-Tu is to 
A.
B.
C.
D.
E.
34.  Please place the steps of translation elongation in the correct order (1) Peptidyl transferase forms a peptide bond betwen the peptide in the P site and the newly arrived aminoacyl-tRNA in the A site (2) EF-G with GTP, translocates peptidyl-tRNA to the P site(3) EF-Tu, with GTP, binds an aminoacyl-tRNA to the ribosomal A site 
A.
B.
C.
D.
E.
35.  Which is correct? 
A.
B.
C.
D.
E.
36.  Which of the following are the three naturally 
A.
B.
C.
D.
E.
37.  Which is false? 
A.
B.
C.
D.
E.
38.  Which of the following enzymes is used to search for CpG island? 
A.
B.
C.
D.
E.
39.  How were the genetic markers that are linked to Huntington disease genes screened originally? 
A.
B.
C.
D.
E.
40.  The binding of which of the following snRNPs to spliceosome requires ATP? 
A.
B.
C.
D.
E.
41.  How were Huntington disease genes identified? 
A.
B.
C.
D.
E.
42.  Regarding to identifying the gene for Huntington disease (HD), which is not correct? 
A.
B.
C.
D.
E.
43.  Which of the following processes was used to locate the gene responsible for Huntington disease? 
A.
B.
C.
D.
E.
44.  With respect to human genome project, which approach is wrong to take? 
A.
B.
C.
D.
E.
45.  Which of the following are techniques used for genomic functional profiling? 
A.
B.
C.
D.
E.
46.  Why proteomics is useful? Because of 
A.
B.
C.
D.
E.
47.  This initiation factor is involved in binding tRNA to the ribosome 
A.
B.
C.
D.
E.
48.  This initiation factor binds to the 40s subunit and inhibits re-association of the 40S and 60s subunits 
A.
B.
C.
D.
E.
49.  This initation factor is a Cap binding protein 
A.
B.
C.
D.
E.
50.  This initiation factor binds to the 60s subunit and inhibits reassociation of the 40S and 60s subunits. 
A.
B.
C.
D.
E.
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