Microbiology Module 4

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Microbiology Quizzes & Trivia
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  • 1. 
    Viruses that infect bacteria are referred to as
    • A. 

      Viralcidens

    • B. 

      Bacteriocidins

    • C. 

      Bacterialogens

    • D. 

      Bacteriophages


  • 2. 
    A virion is a(n)
    • A. 

      Pathogenic virus

    • B. 

      Suviral particle

    • C. 

      Individual virus particle

    • D. 

      Enveloped virus particle


  • 3. 
    A virion is composed of
    • A. 

      Protein,and both RNA and DNA

    • B. 

      Protein, and either RNA or DNA

    • C. 

      Protein

    • D. 

      DNA


  • 4. 
    The protein coat of a virus
    • A. 

      Is called a capsomere

    • B. 

      Is called a capsid

    • C. 

      Protects the nucleic acid

    • D. 

      Is involved in recognition of host cell receptors

    • E. 

      Is called a capsid, protects the nucleic acid AND is involved in recognition of host cell receptors


  • 5. 
    Which does not refer to the shape of a virus?
    • A. 

      Icosahedral (isometric)

    • B. 

      Helical

    • C. 

      Complex

    • D. 

      Bacillus


  • 6. 
    The shape of the virus is determined by its
    • A. 

      Nucleic acid

    • B. 

      Capsid

    • C. 

      Envelope

    • D. 

      Tail


  • 7. 
    The viral envelope closely resembles the
    • A. 

      Prokaryotic cell wall

    • B. 

      Capsomere

    • C. 

      Eukaryotic cell membrane

    • D. 

      Cytoplasm


  • 8. 
    A limiting factor for viral infection is
    • A. 

      Internal metabolic temperature of the host cell

    • B. 

      Nutrition of the host cell

    • C. 

      Stage of cell cycle of the host cell

    • D. 

      Presence of specific receptor molecules on the host cell


  • 9. 
    If the infecting phage lacks some critical pieces of DNA necessary for replication it is called
    • A. 

      Incomplete

    • B. 

      Mutated

    • C. 

      Defective

    • D. 

      Vegetative


  • 10. 
    Specialized transduction
    • A. 

      Involves the random transmission of any gene

    • B. 

      Involves the transfer of a few specific genes

    • C. 

      Utilizes a defective virus

    • D. 

      Only involves genes near the viral DNA integration site

    • E. 

      Involves the transfer of a few specific genes, utilizes a defective virus AND only involves genes near the viral DNA integration site


  • 11. 
    The replicative form of nucleic acid in filamentous phages is
    • A. 

      DsDNA

    • B. 

      DsRNA

    • C. 

      Positive ssRNA

    • D. 

      Negative ssDNA


  • 12. 
    Filamentous phage
    • A. 

      Only infect E.coli that have pili

    • B. 

      Only infect E.coli lacking pili

    • C. 

      Infect E.coli regardless of the presence of pili

    • D. 

      Do not infect E. coli


  • 13. 
    Once inside the host cell, phage DNA
    • A. 

      Is replicated

    • B. 

      Is transcribed

    • C. 

      May get degraded by bacterial nucleases

    • D. 

      All of the choice are correct


  • 14. 
    Most phages that contain single-stranded DNA
    • A. 

      Are extruded

    • B. 

      Contain a positive-sese DNA strand

    • C. 

      Have their DNA transformed to double-stranded DNA before replication and transcription occur

    • D. 

      All of the choice are correct


  • 15. 
    Phages that can either replicate and cause cell lysis or can integrate their DNA into the host DNA are called
    • A. 

      Lysogenic phages

    • B. 

      Lytic phages

    • C. 

      Virulent phages

    • D. 

      Segmented phages


  • 16. 
    One of the most intensively studied virulent phages which infects E.coli is
    • A. 

      T9

    • B. 

      T4

    • C. 

      Beta

    • D. 

      Gamma


  • 17. 
    During attachment of phage to E.coli, the phage
    • A. 

      Actively seek out bacteria

    • B. 

      Randomly bump into bacteria

    • C. 

      Attach to proteins or carbohydrates on the bacterial surface

    • D. 

      Attach to the bacterial RNA

    • E. 

      Randomly bump into the bacteria AND attach to proteins or carbohydrates on the bacterial surface


  • 18. 
    What part of the E. coli T4 phage attaches to the host cell receptors?
    • A. 

      Capsid fragments around the nucleic acid

    • B. 

      Protein fibers at the end of the phage tail

    • C. 

      Pili of the envelope

    • D. 

      Spikes of the envelope


  • 19. 
    During penetration of E. coli by the T4 phage
    • A. 

      Lysozyme is used to allow entry of the phage capsid

    • B. 

      The tail acts as a "hypodermic needle",injecting the phage DNA into the cell

    • C. 

      The protein fibers digest a hole in the cell wall

    • D. 

      The bacterial receptor molecules open a hole through the cell wall


  • 20. 
    Phage-encoded proteins are
    • A. 

      Coded for by host DNA

    • B. 

      Coded for by phage DNA

    • C. 

      Proteins normally present in the uninfected cell

    • D. 

      Early proteins

    • E. 

      Coded for by phage DNA AND early proteins


  • 21. 
    Phage-encoded enzymes are
    • A. 

      All produced simultaneously

    • B. 

      Produced in a sequential manner

    • C. 

      Strictly host enzymes

    • D. 

      Used to customize the cell for viral production

    • E. 

      Produced in a sequential manner AND used to customize the cell for viral production


  • 22. 
    The protein projection on the surface of a virus that are involved in attachment to the host cell are called
    • A. 

      Suckers

    • B. 

      Pili

    • C. 

      Cilia

    • D. 

      Spikes

    • E. 

      Hooks


  • 23. 
    Outside of living cells, viruses are
    • A. 

      Scavenging glucose

    • B. 

      Slowly stockpiling ATP from the mitochondria

    • C. 

      Using cilia to move to the next host

    • D. 

      Metabotically inert


  • 24. 
    Viruses
    • A. 

      Probably keep the number of bacteria in check

    • B. 

      Habe no effect on the number of bacteria

    • C. 

      Increase the number of bacteria

    • D. 

      Are active in passing DNA from one bacterium to another

    • E. 

      Probably keep the numbers of bacteria in check AND are active in passing DNA from one bacterium to another


  • 25. 
    What part of the attached bacteriophage enters through the host cell wall?
    • A. 

      The entire virus

    • B. 

      Only the enzymes necessary for replication

    • C. 

      The nucleic acid

    • D. 

      The nucleic acid and capsid

    • E. 

      The capsid only


  • 26. 
    A phage that replicates inside the host cell and then lyses its host during its release is a
    • A. 

      Virulent or lytic phage

    • B. 

      Latent phage

    • C. 

      Lysogenic phage

    • D. 

      Dormant phage


  • 27. 
    The correct order for the stages of a phage infection are
    • A. 

      Penetration,transcription,attachment,replication of nucleic acid and protein,assembly,release

    • B. 

      Attachment,penetration,transcription,replication of nucleic acid and protein,assembly,release

    • C. 

      Attachment,replication of nucleic acid and protein penetration,transcription,assembly,release

    • D. 

      Transcription, attachment replication of nucelic acid and protein,assembly,penetration,release.


  • 28. 
    Once integrated,phage DNA can remain in the prophage state as long as
    • A. 

      The bacteria is frequently plated on new media

    • B. 

      Certain phage genes are excised

    • C. 

      Certain phage genes are expressed

    • D. 

      Bacterial repressor genes are activated


  • 29. 
    Lysogenic cells
    • A. 

      Are immune to any further infection by any virus

    • B. 

      Are immune to infection by the same virus

    • C. 

      May have new properties

    • D. 

      Respond to infection with the SOS response

    • E. 

      Are immune to infection by the same virus AND may have new properties


  • 30. 
    The filamentous phages all contain
    • A. 

      Single-stranded DNA

    • B. 

      Double-stranded DNA

    • C. 

      Single-stranded RNA

    • D. 

      Double-stranded RNA


  • 31. 
    Bacteria infected with filamentous phages are termed
    • A. 

      Temperate cells

    • B. 

      Plaque-producing cells.

    • C. 

      Virulent strains

    • D. 

      Carrier cells


  • 32. 
    Transducing virulent phages do not lyse the cells they invade because
    • A. 

      Transformation is taking place in the phage and this is transferred to the bacterium

    • B. 

      Bacterial DNA hass replaced critical viral DNA in the phage

    • C. 

      Their virulence is dependent on the bacteria and virus replicating together

    • D. 

      The lytic genes are unable to enter during penetration and are shed outside the host


  • 33. 
    The groups of organisms that a virus may infect is known as its
    • A. 

      Target circle

    • B. 

      Host range

    • C. 

      Susceptible group

    • D. 

      Receptor type


  • 34. 
    DNA is protected from restriction enzymes by being
    • A. 

      Sequestered in a lysosome

    • B. 

      Turned into RNA

    • C. 

      Methylated

    • D. 

      Made into double stranded RNA


  • 35. 
    Assembly of the T4 phage
    • A. 

      May involve some self-assembly

    • B. 

      May involve the use of scaffolds

    • C. 

      Is completely self-assembly

    • D. 

      Is completely dependent on scaffolds

    • E. 

      May involve some self assembly AND may involve the use of scaffolds


  • 36. 
    An exit method used by viruses which does not immediately destroy the host bacterium is
    • A. 

      Lysis

    • B. 

      Inversion

    • C. 

      Extrusion

    • D. 

      Excising


  • 37. 
    In the replication of phage containing positive sense DNA,
    • A. 

      The host's enzymes are used to make dsDNA

    • B. 

      The host's DNA polymerase uses the phage RNA as a template to make negative-sense DNA

    • C. 

      A phage-encoded DNA polymerase is used to make negative sense RNA using the phage positive-sense RNA as a template

    • D. 

      A phage-encoded DNA polymerase is used to make DNA using the phage positive-sense RNA as a template


  • 38. 
    RNA phages usually contain
    • A. 

      DsRNA

    • B. 

      DsDNA

    • C. 

      SsRNA

    • D. 

      SsDNA


  • 39. 
    Regarding phage replication,
    • A. 

      The majority of phages are temperate

    • B. 

      When intergrated into host DNA, the phage DNA is called a prophage

    • C. 

      Lambda is a good example of a temperate phage

    • D. 

      All of the choice are correct


  • 40. 
    A temperate phage
    • A. 

      May be lysogenic

    • B. 

      May be lytic

    • C. 

      Enters a lysogenic or lytic life cycle shortly after entering the host cell

    • D. 

      Are all RNA viruses

    • E. 

      May be lysogenic AND enters a lysogenic or lytic life cycle shortly after entering the host cell


  • 41. 
    The integration of phage DNA into the bacterial chromosome occurs because of
    • A. 

      Identical DNA sequences in both

    • B. 

      The phages ability to synthesize enzymes to enter the bacterium

    • C. 

      Similar RNA nucleotides in both

    • D. 

      The similarity in enzyme metabolism

    • E. 

      The phages ability to synthesize enzymes to enter the bacterium AND similar RNA nucleotides in both


  • 42. 
    What is a single Virus Particle Called?

  • 43. 
    What does the lytic cycle cause?

  • 44. 
    What activates the lysogenic production of prophage DNA?

  • 45. 
    What is the proper sequence of animal replication in animal cells?
    • A. 

      Transmission, attachment, entry of the virion, targeting,uncoating,replication, maturation,release,shedding

    • B. 

      Attachment,entry of the virion,targeting,uncoating,replication,maturation,release,shedding,transmission

    • C. 

      Attachment,entry of the virion, uncoating, replication,release,shedding,transmission, and targeting

    • D. 

      Attachment, transmission,release,uncoating,entry of the virus, targeting,maturation, release,shedding


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