Microbiology Exam II (chapter 8 )

58 Questions  I  By Ekanye
These are quiz question derived from the notes and book good luck! These Quiz Question only cover Chapter 8

  
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1.  Diversity in a bacteria can be generated via..
A.
B.
C.
2.  Modification in the sequence of DNA is a gene often resulting in an alteration in the protein encoded gene is known as..
A.
B.
C.
D.
3.  Define Mutation
A.
B.
C.
4.  What are mutations that occur in the cell's natural environment
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B.
C.
5.  Spontaneous Mutations are...
A.
B.
C.
6.  Vertical gene transfer
A.
B.
C.
7.  Reversion are...
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B.
C.
8.  Spontaneous mutation can occur as a result of what?...
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B.
C.
D.
E.
9.  Base Substitution are.
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B.
C.
D.
E.
10.  Point Mutations
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B.
C.
D.
11.  Missense mutation
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B.
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D.
12.  Nonsense mutation
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B.
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D.
13.  Null or knockout mutation
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D.
14.  What does the removal or addition of Nucleotides do?
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B.
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D.
E.
15.  What are the effects of a frameshift mutation?
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D.
E.
16.  What are transposable elements
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B.
C.
17.  Another name for Transposable elements
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18.  What happens to the gene after transposon inserts..
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E.
19.  What are induced mutations?
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20.  Mutations can be induced via...
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E.
21.  Define chemical Mutagens
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22.  What are Base Analogs...
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23.  What are intercalating agents?
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B.
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D.
24.  What is a common intercalating agent?
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25.  Characteristic of Ethidium Bromide
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B.
C.
D.
26.  Transpositon...
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B.
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D.
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27.  Genes that move from one replicon to another site in the same replicon, or to another replicon on the same cell
A.
B.
C.
D.
E.
28.  Genes that receives the transposon will undergo a knockout mutation termed?
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B.
C.
D.
E.
29.  Name two type of Radiaton in Induced Mutations
A.
B.
C.
D.
E.
30.  Define Ultraviolet light * (please choose three of the correct answers)
A.
B.
C.
D.
E.
31.  Define X-rays (please choose two of the correct answer)
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B.
C.
D.
E.
32.  Major problem in induced mutation is identifying bacteria with desired mutation, name the techniques used...
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B.
C.
D.
E.
33.  Define Direct Selection...
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B.
C.
D.
34.  Indirect selection...
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35.  Testing for cancer causing chemical (carcinogens), many mutagens are also carcinogens, microbes used to test potential carcinogenic activity, test are based on effect chemicals has on mibrobial DNA. What is a common chemical carcinogen test?
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B.
C.
D.
E.
36.  Ames test (check all that are correct/ more than one can be correct)
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B.
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D.
E.
37.  Genes transferred from one cell to another...
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B.
C.
D.
38.  Genes are naturally transferred between bacteria using mechanisms what are the mechanisms?
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D.
E.
39.  Involves the transfer of naked DNA from the environment to the recipent cell, cells rupture during the stationary and death phase, the chromosome breaks into small pieces and explodes through the ruptured cell wall, recipeitn "competent" cell picks up piece of the naked DNA, the naked DNA is integrated onto the recipeint chromosome, (replaces the homologous gene on the cromose to the recipeint cell). What type mechanisms of Gene transfer is that?
A.
B.
C.
40.  DNA-mediated transformation; natural transformation occurs when bacterial cells are compentent, bacterial cells are capable of taking up and integrating larger fragments of DNA. Natural transformtion occurs in stages what are they. (choose all that apply)
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B.
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D.
E.
41.  In Natural transformation this "stage"... only single strands enter, double strands are degraded
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B.
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D.
E.
42.  In Natrural transformation this "stage"... donor DNA is integrated by hydrogen bonding, enzymes cleave recipient DNA, Donor DNA is put in place
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B.
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D.
E.
43.  In Natural transformation this "stage"... repair mechanism removes either donor or recipient DNA that doesn't match, repairs with correct nucleotides
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44.  In Natural transformation this "stage"... transformed cells multiply under selective conditions in which non-transfored cells will not grow
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E.
45.  Mechanisms of Gene Transfer... Bacterial DNA that is transferred from donor to recipeint via a bacterial virus (bacteriophage)
A.
B.
C.
D.
46.  Is a mispackaging of DNA during viral replication, the mis-packaged phage infects a new bacterial cell and inserts the donor DNA inot the recipient cell, the donor DNA is integrated into the cell by homologous recombination. Is what type of mechanisms of gene transfer?
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B.
C.
D.
47.  Mechanisms of Gene transfer; only form ofgene exchagne in which donor survives
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B.
C.
D.
48.  Conjuagtion is mediated by a plasmid
A.
B.
49.  Plasmid is self replication extrachromosomal piece of DNA (can code for traits that give bacteria advantage)
A.
B.
50.  Conjugation requires direct contact between cells, cells must be of opposite mating types
A.
B.
51.  Mechanisms of Gene Transfer: Group of plasmids that confer resistance to many antimicrobial agents
A.
B.
C.
D.
52.  Self- transmissible
A.
B.
53.  Mobilizable
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B.
54.  During conjugation, the plasmid is replicated in the donor cell and is transferred to the recipient
A.
B.
55.  After, plasmid is transferred F-cell becomes F+ some cells can be cured, spontaneously lose thier plasmid.
A.
B.
56.  In some F+ cell, the F factor integrates onto the host chromosome (converts F+ Hfr)
A.
B.
57.  Conjuagtion between Hfr adn F- cell results in only a portion of the F factor being replicated and transferred (F- remain F-, F- has new information but may not have the F factor gene)
A.
B.
58.  Plasmid of HFr strain can be excised from chromosome resulting in the excision of a small section of the bacterial chromosome, has transfer properties of the F plasmid but carries chromosomal DNA.
A.
B.
C.
D.
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