Microbiology: Chapter 3

50 Questions  I  By Kaiello

  
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1.  Which of these must be done prior to staining?
A.
B.
C.
2.  If a microscope magnifies a specimen by 100X. Does this mean the object looks 100 times as large as it really is?
A.
B.
3.  The definition for the gram stain is...
A.
B.
C.
4.  How small are they? A Virus is what?
A.
B.
C.
5.  The gram stain was developed by Hans Christian Gram in 1884 in order to aid in bacterial identification?
A.
B.
6.  What is a reason that after a gram stain procedure, some bacteria react differently? 
A.
B.
C.
7.  Which of these is a correct statement for resolution?
A.
B.
C.
8.  Which of these is the third step in performing a gram stain procedure? 
A.
B.
C.
D.
9.  Which of these is the final step in a gram staining procedure?
A.
B.
C.
D.
10.  Is it True that Electron microscopy has three types?
A.
B.
11.  What is the maximum resolving power and maximum magnification for a compound microscope?
A.
B.
C.
D.
12.  Safranin colors the entire bacterium except for the (blank)l
13.  Which of the following are staining techniques? 
A.
B.
C.
D.
E.
14.  Resolving power has to do with
A.
B.
C.
15.  Capsules are relate to the (blank) of pathogens.
16.  An official definition, as stated here; "the ability of an optical system to distinguish between two adjacent points" relates to what? 
A.
B.
C.
17.  After a gram stain procedure is complete, gram-positive cells should look purple and gram-negative cells look red. 
A.
B.
18.  In this type of microscopy, a specimen appears light against a dark background and requires no staining. This is what?
A.
B.
C.
D.
19.  Which is the third step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
20.  Which is the fourth step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
21.  In this type of microscopy, a specimen appears bight against a dark background, and is often used for diagnostic purposes. This is what?
A.
B.
C.
D.
22.  The definition for a simple stain is...
A.
B.
C.
23.  Which stain, preferentially distinguishes bacteria of the genus Mycobacterium, which causes tuberculosis?
A.
B.
C.
D.
24.  Dyes are salts composed of which of the following?
A.
B.
C.
D.
E.
25.  Which of these are common objective lenses found on a compound microscope? (Choose all that apply)
A.
B.
C.
D.
E.
26.  What two specific things are increased when specimens observed under a compound microscope are stained?
A.
B.
C.
27.  The definition for a differential stain is...
A.
B.
C.
28.  How small are they? A eukaryotic cells is what?
A.
B.
C.
29.  Special stains are used to...
A.
B.
C.
D.
E.
F.
30.  Which of these is the second step in performing a gram stain procedure? 
A.
B.
C.
D.
31.  How small are they? A bacteria is what?
A.
B.
C.
32.  Which choice best fits this description? Electrons bounce off the surface of the specimen, revealing a three-dimensional image, has the best pictures and magnifications are normally 10,000X, resolution 20nM, this is similar in idea to darkfield microscopy
A.
B.
33.  Which choice best fits this description? Beam penetrates sample just like in light microscopy, samples must be very thin, the highest magnification and resolution is up to 1,000,000 x, 1nM, and is similar in idea to brightfield microscopy
A.
B.
34.  In Electron microscopy...(Choose all that apply)
A.
B.
C.
D.
E.
35.  Because bacteria are somewhat negatively charged, basic dyes....
A.
B.
C.
36.  Which is a correct statement for magnification?
A.
B.
C.
37.  Which of these is the first step in performing a gram stain procedure? 
A.
B.
C.
D.
38.  Which of these relates to the statement "chromophore is negative ion."
A.
B.
39.  Immersion oil must be used under high magnification to/because... (Choose all that apply)
A.
B.
C.
D.
40.  Which is the second step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
41.  Which is the first step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
42.  What are the two types of Electron microscopy? 
43.  What is another word for when acidic dyes cause a negative staining with bacteria?
44.  Which of the following types of staining techniques will improve binding of a dye to the sample with use of Mordant?
A.
B.
C.
D.
E.
45.  Upon completion of an Acid-fast stain, acid-fast cells look red, and non acid-fast cells are yellow.
A.
B.
46.  Because bacteria are somewhat negatively charges, acidic dyes...
A.
B.
C.
D.
47.  Specimens are stained with "fluorochromes", which absorb and radiate UV light in Fluorescence microscopy. (T/F)
A.
B.
48.  The compound light microscope uses visible light.
A.
B.
49.  Which of these dyes relates to the statement "chromophore is positive ion."
A.
B.
50.  Is it true that magnification can be expressed as a number combined with X (times)?
A.
B.
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