Microbiology: Chapter 3

50 Questions  I  By Kaiello

  
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1.  Which is the second step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
2.  Immersion oil must be used under high magnification to/because... (Choose all that apply)
A.
B.
C.
D.
3.  Is it True that Electron microscopy has three types?
A.
B.
4.  How small are they? A Virus is what?
A.
B.
C.
5.  Specimens are stained with "fluorochromes", which absorb and radiate UV light in Fluorescence microscopy. (T/F)
A.
B.
6.  Which is a correct statement for magnification?
A.
B.
C.
7.  Because bacteria are somewhat negatively charged, basic dyes....
A.
B.
C.
8.  Which of these is the first step in performing a gram stain procedure? 
A.
B.
C.
D.
9.  Special stains are used to...
A.
B.
C.
D.
E.
F.
10.  Which stain, preferentially distinguishes bacteria of the genus Mycobacterium, which causes tuberculosis?
A.
B.
C.
D.
11.  Because bacteria are somewhat negatively charges, acidic dyes...
A.
B.
C.
D.
12.  The compound light microscope uses visible light.
A.
B.
13.  An official definition, as stated here; "the ability of an optical system to distinguish between two adjacent points" relates to what? 
A.
B.
C.
14.  Safranin colors the entire bacterium except for the (blank)l
15.  What are the two types of Electron microscopy? 
16.  Which choice best fits this description? Beam penetrates sample just like in light microscopy, samples must be very thin, the highest magnification and resolution is up to 1,000,000 x, 1nM, and is similar in idea to brightfield microscopy
A.
B.
17.  Which of these must be done prior to staining?
A.
B.
C.
18.  In this type of microscopy, a specimen appears light against a dark background and requires no staining. This is what?
A.
B.
C.
D.
19.  Which is the third step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
20.  How small are they? A bacteria is what?
A.
B.
C.
21.  Which of these is the final step in a gram staining procedure?
A.
B.
C.
D.
22.  Which of these is the third step in performing a gram stain procedure? 
A.
B.
C.
D.
23.  Which of these relates to the statement "chromophore is negative ion."
A.
B.
24.  The definition for the gram stain is...
A.
B.
C.
25.  Which of these are common objective lenses found on a compound microscope? (Choose all that apply)
A.
B.
C.
D.
E.
26.  Which of these is the second step in performing a gram stain procedure? 
A.
B.
C.
D.
27.  Which of these is a correct statement for resolution?
A.
B.
C.
28.  Capsules are relate to the (blank) of pathogens.
29.  Resolving power has to do with
A.
B.
C.
30.  In this type of microscopy, a specimen appears bight against a dark background, and is often used for diagnostic purposes. This is what?
A.
B.
C.
D.
31.  Which choice best fits this description? Electrons bounce off the surface of the specimen, revealing a three-dimensional image, has the best pictures and magnifications are normally 10,000X, resolution 20nM, this is similar in idea to darkfield microscopy
A.
B.
32.  Is it true that magnification can be expressed as a number combined with X (times)?
A.
B.
33.  How small are they? A eukaryotic cells is what?
A.
B.
C.
34.  In Electron microscopy...(Choose all that apply)
A.
B.
C.
D.
E.
35.  What is a reason that after a gram stain procedure, some bacteria react differently? 
A.
B.
C.
36.  The gram stain was developed by Hans Christian Gram in 1884 in order to aid in bacterial identification?
A.
B.
37.  Which of these dyes relates to the statement "chromophore is positive ion."
A.
B.
38.  Dyes are salts composed of which of the following?
A.
B.
C.
D.
E.
39.  After a gram stain procedure is complete, gram-positive cells should look purple and gram-negative cells look red. 
A.
B.
40.  Which is the fourth step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
41.  Which of the following types of staining techniques will improve binding of a dye to the sample with use of Mordant?
A.
B.
C.
D.
E.
42.  The definition for a differential stain is...
A.
B.
C.
43.  If a microscope magnifies a specimen by 100X. Does this mean the object looks 100 times as large as it really is?
A.
B.
44.  What is the maximum resolving power and maximum magnification for a compound microscope?
A.
B.
C.
D.
45.  Which is the first step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
46.  The definition for a simple stain is...
A.
B.
C.
47.  Upon completion of an Acid-fast stain, acid-fast cells look red, and non acid-fast cells are yellow.
A.
B.
48.  What is another word for when acidic dyes cause a negative staining with bacteria?
49.  Which of the following are staining techniques? 
A.
B.
C.
D.
E.
50.  What two specific things are increased when specimens observed under a compound microscope are stained?
A.
B.
C.
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