Microbiology: Chapter 3

50 Questions  I  By Kaiello

  
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1.  The definition for a simple stain is...
A.
B.
C.
2.  Specimens are stained with "fluorochromes", which absorb and radiate UV light in Fluorescence microscopy. (T/F)
A.
B.
3.  How small are they? A Virus is what?
A.
B.
C.
4.  What are the two types of Electron microscopy? 
5.  In this type of microscopy, a specimen appears light against a dark background and requires no staining. This is what?
A.
B.
C.
D.
6.  Which of the following types of staining techniques will improve binding of a dye to the sample with use of Mordant?
A.
B.
C.
D.
E.
7.  What is another word for when acidic dyes cause a negative staining with bacteria?
8.  Which of these relates to the statement "chromophore is negative ion."
A.
B.
9.  After a gram stain procedure is complete, gram-positive cells should look purple and gram-negative cells look red. 
A.
B.
10.  Because bacteria are somewhat negatively charged, basic dyes....
A.
B.
C.
11.  What two specific things are increased when specimens observed under a compound microscope are stained?
A.
B.
C.
12.  Is it true that magnification can be expressed as a number combined with X (times)?
A.
B.
13.  Which of these dyes relates to the statement "chromophore is positive ion."
A.
B.
14.  In this type of microscopy, a specimen appears bight against a dark background, and is often used for diagnostic purposes. This is what?
A.
B.
C.
D.
15.  An official definition, as stated here; "the ability of an optical system to distinguish between two adjacent points" relates to what? 
A.
B.
C.
16.  Safranin colors the entire bacterium except for the (blank)l
17.  Is it True that Electron microscopy has three types?
A.
B.
18.  Special stains are used to...
A.
B.
C.
D.
E.
F.
19.  Which of these is the third step in performing a gram stain procedure? 
A.
B.
C.
D.
20.  Which is the fourth step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
21.  Immersion oil must be used under high magnification to/because... (Choose all that apply)
A.
B.
C.
D.
22.  Which choice best fits this description? Electrons bounce off the surface of the specimen, revealing a three-dimensional image, has the best pictures and magnifications are normally 10,000X, resolution 20nM, this is similar in idea to darkfield microscopy
A.
B.
23.  How small are they? A bacteria is what?
A.
B.
C.
24.  Which of these is the first step in performing a gram stain procedure? 
A.
B.
C.
D.
25.  How small are they? A eukaryotic cells is what?
A.
B.
C.
26.  Which of these are common objective lenses found on a compound microscope? (Choose all that apply)
A.
B.
C.
D.
E.
27.  The gram stain was developed by Hans Christian Gram in 1884 in order to aid in bacterial identification?
A.
B.
28.  Because bacteria are somewhat negatively charges, acidic dyes...
A.
B.
C.
D.
29.  Which is a correct statement for magnification?
A.
B.
C.
30.  Which of these is the final step in a gram staining procedure?
A.
B.
C.
D.
31.  Dyes are salts composed of which of the following?
A.
B.
C.
D.
E.
32.  Which is the second step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
33.  Which of these must be done prior to staining?
A.
B.
C.
34.  Upon completion of an Acid-fast stain, acid-fast cells look red, and non acid-fast cells are yellow.
A.
B.
35.  Resolving power has to do with
A.
B.
C.
36.  What is a reason that after a gram stain procedure, some bacteria react differently? 
A.
B.
C.
37.  Which of these is a correct statement for resolution?
A.
B.
C.
38.  Which is the first step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
39.  Which is the third step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
40.  The compound light microscope uses visible light.
A.
B.
41.  Which stain, preferentially distinguishes bacteria of the genus Mycobacterium, which causes tuberculosis?
A.
B.
C.
D.
42.  Which of the following are staining techniques? 
A.
B.
C.
D.
E.
43.  The definition for a differential stain is...
A.
B.
C.
44.  In Electron microscopy...(Choose all that apply)
A.
B.
C.
D.
E.
45.  Which of these is the second step in performing a gram stain procedure? 
A.
B.
C.
D.
46.  Which choice best fits this description? Beam penetrates sample just like in light microscopy, samples must be very thin, the highest magnification and resolution is up to 1,000,000 x, 1nM, and is similar in idea to brightfield microscopy
A.
B.
47.  The definition for the gram stain is...
A.
B.
C.
48.  If a microscope magnifies a specimen by 100X. Does this mean the object looks 100 times as large as it really is?
A.
B.
49.  Capsules are relate to the (blank) of pathogens.
50.  What is the maximum resolving power and maximum magnification for a compound microscope?
A.
B.
C.
D.
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