Microbiology: Chapter 3

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1.  Which is the third step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
2.  Safranin colors the entire bacterium except for the (blank)l
3.  Which is the second step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
4.  Which of these is a correct statement for resolution?
A.
B.
C.
5.  What is the maximum resolving power and maximum magnification for a compound microscope?
A.
B.
C.
D.
6.  How small are they? A Virus is what?
A.
B.
C.
7.  Dyes are salts composed of which of the following?
A.
B.
C.
D.
E.
8.  In this type of microscopy, a specimen appears bight against a dark background, and is often used for diagnostic purposes. This is what?
A.
B.
C.
D.
9.  Which is the first step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
10.  What is another word for when acidic dyes cause a negative staining with bacteria?
11.  Which of these is the first step in performing a gram stain procedure? 
A.
B.
C.
D.
12.  Which stain, preferentially distinguishes bacteria of the genus Mycobacterium, which causes tuberculosis?
A.
B.
C.
D.
13.  Specimens are stained with "fluorochromes", which absorb and radiate UV light in Fluorescence microscopy. (T/F)
A.
B.
14.  Which of these relates to the statement "chromophore is negative ion."
A.
B.
15.  The definition for a differential stain is...
A.
B.
C.
16.  How small are they? A eukaryotic cells is what?
A.
B.
C.
17.  After a gram stain procedure is complete, gram-positive cells should look purple and gram-negative cells look red. 
A.
B.
18.  The gram stain was developed by Hans Christian Gram in 1884 in order to aid in bacterial identification?
A.
B.
19.  Because bacteria are somewhat negatively charges, acidic dyes...
A.
B.
C.
D.
20.  Which of these is the final step in a gram staining procedure?
A.
B.
C.
D.
21.  Which choice best fits this description? Electrons bounce off the surface of the specimen, revealing a three-dimensional image, has the best pictures and magnifications are normally 10,000X, resolution 20nM, this is similar in idea to darkfield microscopy
A.
B.
22.  Which choice best fits this description? Beam penetrates sample just like in light microscopy, samples must be very thin, the highest magnification and resolution is up to 1,000,000 x, 1nM, and is similar in idea to brightfield microscopy
A.
B.
23.  In this type of microscopy, a specimen appears light against a dark background and requires no staining. This is what?
A.
B.
C.
D.
24.  Which of these is the third step in performing a gram stain procedure? 
A.
B.
C.
D.
25.  An official definition, as stated here; "the ability of an optical system to distinguish between two adjacent points" relates to what? 
A.
B.
C.
26.  Which of the following types of staining techniques will improve binding of a dye to the sample with use of Mordant?
A.
B.
C.
D.
E.
27.  What are the two types of Electron microscopy? 
28.  If a microscope magnifies a specimen by 100X. Does this mean the object looks 100 times as large as it really is?
A.
B.
29.  Because bacteria are somewhat negatively charged, basic dyes....
A.
B.
C.
30.  What is a reason that after a gram stain procedure, some bacteria react differently? 
A.
B.
C.
31.  Which of the following are staining techniques? 
A.
B.
C.
D.
E.
32.  Is it true that magnification can be expressed as a number combined with X (times)?
A.
B.
33.  Resolving power has to do with
A.
B.
C.
34.  Which is the fourth step in the procedure for an Acid-fast stain?
A.
B.
C.
D.
35.  Immersion oil must be used under high magnification to/because... (Choose all that apply)
A.
B.
C.
D.
36.  Special stains are used to...
A.
B.
C.
D.
E.
F.
37.  The compound light microscope uses visible light.
A.
B.
38.  Capsules are relate to the (blank) of pathogens.
39.  What two specific things are increased when specimens observed under a compound microscope are stained?
A.
B.
C.
40.  Which of these is the second step in performing a gram stain procedure? 
A.
B.
C.
D.
41.  The definition for a simple stain is...
A.
B.
C.
42.  Upon completion of an Acid-fast stain, acid-fast cells look red, and non acid-fast cells are yellow.
A.
B.
43.  Is it True that Electron microscopy has three types?
A.
B.
44.  Which of these must be done prior to staining?
A.
B.
C.
45.  The definition for the gram stain is...
A.
B.
C.
46.  Which of these are common objective lenses found on a compound microscope? (Choose all that apply)
A.
B.
C.
D.
E.
47.  Which of these dyes relates to the statement "chromophore is positive ion."
A.
B.
48.  How small are they? A bacteria is what?
A.
B.
C.
49.  Which is a correct statement for magnification?
A.
B.
C.
50.  In Electron microscopy...(Choose all that apply)
A.
B.
C.
D.
E.
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