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Catalyze chemical reactions
With only four types of nucleotides, it was a very simple, repetitive molecule that could not account for 20 different amino acids.
Is had been shown that proteins were more important in transferring genetic information than nucleic acids.
Proteins and carbohydrates were of more interest.
It was thought that RNA was more likely to be the genetic material.
Transfer of genes between two bacteria.
infection with a virus called bacteriophage
Production of a cancer cell.
The mating of two different kinds of live bacteria.
Watson and Crick
Sulfur is present in amino acids in the protein coat of bacteria.
Phosphorus is present in high amounts in DNA.
Sulfur is not present in DNA
Phosphorus is not present in amino acids in the protein coat of bacteria.
All of the above were critical to demonstrating DNA is involved in genetic material.
A + T = G + C
A + G = T + C
A = G, T = C
A = C, T = G
Indicating that DNA has a double helix structure.
Showing equal number of purines and pyrimidines.
Showing the bases of DNA were held together by hydrogen bonds.
Revealing the structure of the deoxyribose sugar.
Of the location of each adenine, guanine, cytosine, and thymine.
A purine and a pyrimidine
A sugar and a phosphate molecule
Unzipping of the DNA molecule occurs as hydrogen bonds break.
Replication occurs as each base is paired with another exactly like it.
The process is known as semiconservative replication because one old strand is conserved in the new molecule.
The enzyme that catalyzes DNA replication is DNA polymerase.
Complementary base pairs are held together with hydrogen bonds.
Recognize the free nucleotide that pairs with the base on the template strand of DNA
Read the strand of template DNA and recognize the base there
Proofread to ensure that the proper base has been incorporated
Make the proper nucleotide to match with the base read on the template strand
Cut out an improperly paired nucleotide and replace it with the proper one
) It proceeds in a 5' to 3' direction only
One strand of new DNA is replicated faster than the other strand at the replication fork.
DNA can only replicate at one point on a chromosome at one time.
It occurs more rapidly in bacteria than in eukaryotes.
Replication can only begin at a special origin of replication.
Replication begins at a single origin of replication.
Replication is bidirectional from the origin(s).
Replication occurs at about 1 million base pairs per minute.
Since bacterial cells replicate so rapidly, a second round of replication may begin before the first has completed.
There are numerous different bacterial chromosomes, with replication occurring in each at the same time.
Long strands of nucleotides
A "backbone" of sugar and phosphate