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Name 2 types of stains for staining retics |
New methylene Blue, Brilliant Cresyl Blue |
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What is the equation for a manual platelet count? |
number of plts counted x 1000 (x10^9) |
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When would a manual WBC or platelet count be done? |
Body fluid analysis (CSF, pleural fluid, etc), Errors or flags from automated instruments,
Interfering substances |
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What is a supravital stain? |
Stains residual RNA in the cytoplasm of Retics |
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Which optics are used for counting Retics, WBCs and RBCs manually? |
WBC: x10,
RBCs: x40,
Platelets: x40 |
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What are 4 common sources of error in manual counting methods? |
Incorrect dilution,
Faulty equipment,
Technique (over or under filling the chamber),
Nature of the sample (no clots, thoroughly mixed) |
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What area on a hemocytometer is used for WBC counts? |
All 9 large squares |
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What is the equation for a manual WBC count? |
Total WBC (mm3) = WBCs counted x 10 x 100/9
Or Add 10% to the total # WBCs counted and multiply by 100 |
