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Step one (beginning of energy investment phase)
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Glucose is phosphorylated and thus energized by hexokinase, using 1ATP. More reactive now.
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Step two
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Glucose-6-phosphate is rearrange by phosphoglucoisomerase and converted to fructose-6-phosphate
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Step three
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Another ATP is added by phosphofructokinase. Sugar now very unstable and has phosphate groups at each end
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Step four
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Sugar splits (aldolase) into 3-C sugars: PGAL, and its isomer dihydroxyacetone phosphate (basically 2 PGALs)
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Step five
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Enzyme catalyzes the conversion of dihydroxyacetone phosphate to PGAL bc the next enz. in glycolysis is unreceptive to isomer.
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Step six (beginning of energy yielding phase)
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2reactions by 1enz. 1: Sugar is oxidized, forming NADH, v. exergonic. (REMOVES) 2: Phosp. grp is attached to oxidized substrate (ADDS)
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Step seven (by end of this, 2 ATPs per glucose are made, zero net gain)
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Phos. grp. just added is removed and joined to ADP (substrate level phosp.). remain 3-phosphoglycerate is an acid.
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Step eight
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Enz. relocates the remaining phosphate group in order to prepar the substrate for the next rxn
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Step nine
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An enzyme forms a double bond in the substrate by removing H2O. Makes phosphoenolpyruvate (PEP)
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Step ten
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PEP transfers a phosphate group to ADP to make ATP, pyruvate is left. this is called substrate level phosphorylation
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