Staphylococcus and Micrococcus

Staphylococcus and Micrococcus epidemiology, morphology, culture characteristics, diagnostic biochemical tests and serology.

35 cards   |   Total Attempts: 183
  

Cards In This Set

Front Back
What is the Gram and microscopic arrangement of both staph and micrococcus?
Gram +Cocci in clusters, pairs or tetrads
Are staph and micrococcus catalase positive or negative?
Positive
What sites of infection are staph infections associated with?
Various skin and mucosal surfaces when strain gains entrance to normally sterile site
Is S. aureus coagulase negative or positive?
Positive
What are 3 diseases associated with Staph aureus?
Impetigo - skin infectionScalded skin syndrome - affects babiesToxic shock syndrome - systemic
What 3 species of Staph are assoc. with nosocomial infections other than S. aureus?
S. epidermidisS. haemolyticusS. lugdunenis
Are S. epidermidus, S. haemolyticus, and S. lugdunenis coag neg or pos?
Negative
What two characteristics of S. epidermidis enhances its likelihood of infection?
Its production of an exopolysaccharide that enhances attachment and its ability to acquire resistance to most antimicrobial agents used in the hospital
How is S. lugdunensis differentiated from other coag neg staph from sterile sites?
PYR pos and pos for ornithine decarboxylase
Why does S. lugdunensis need to be differentiated from other coag neg staph from sterile sites?
Because it has different interpretative criteria for oxacillin
What infection is S. saprophyticus most commonly assoc. with?
Community acquired urinary tract infections in young sexually active females
What media can be used to cultivate staph and micrococcus?
5% sheep blood agar, Chocolate agar, broth blood cultures, Nutrient broths
What selective media is used to isolate staph?
Mannitol salt agar
What would a colony with a yellow halo on an MSA plate indicate?
That it is a staph colony that can ferment mannitol
What are the incubation conditions to cultivate staph on BAP, chocolate and MSA?
BAP and Chocolate - 35oC in CO2 or ambient air from 24 hrMSA 48-72 hr