Phlebotomy 5 - Laboratory, Order Of Draw, "Test Tubes, Additives And Tests"

For The Test On Friday
Created Jun 24, 2009
by tsablay
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Side ASide B
The process by which blood vessels are repaired after an injury. It starts from vascular contraction...
Four stages of Hemostatis
1. Vascular phase2. Platelet phase3. Coagulation phase4. Fibrinolysis
Stage one of hemostatis
Injury to a blood vessel causes it to constrict, slowing the flow of blood
Stage two of hemostatis
Injury to the endothelial lining causes platelets to adhere to it.Additional platelets stick...
Stage three - coagulation phase
Involves a cascade of interactions of coagulation factors that converts the temporary platelet...
Stage four - Fibrinolysis
This is the breakdown and removal of the clot. As tissue repair starts, plasmin (an enzyme)...
Warmed Specimen
Some tests require that the specimen collected be kept warm until the serum is separated from...
Chilled Specimens
Some test require that the specimen collected be chilled immediately after collection in an...
Light-sensitive specimens
wrap in aluminum foild immediately after they are drawn.Exposure to light coudl alter the test...
Cold Agglutinins
C.A. are antibodies produced in response to Mycoplasma pneumoniae infection (atypical pneumonia)....
Chilled specimens
Some tests require that the specimen collected be chilled immediately after collection in crushed...
Specimens that require Chilling
- ammonia- Arterial Blood Gases (also determine PH)- Gastrin - Glucagon- Lactic Acid- Parathyroid...
Specimens that require protection from Light
- Bilirubin-Carotene-Red Cell Folate- Serum Folate- Urine Porphyrins- Vitamin B12
Specimens requiring 37 degree Celsius Collection and transportation
- Cold agglutinin- Cryofibrinogen- Cryoglobulin (Cryoprotein)
Order of draw recommended by National Healthcareer Association
1. blood culture or vials2. sodium citrate tubes (e.g. blue tops)3. Serum tubes with or without...
what should you do if a light blue is the only tube to be drawn
draw a red top tube first to eliminate potential contamination from the skin or needle.This...
Color Tube for EDTA
Lavender top
Color tube for heparin
Green top
color for Serum tube with or without clot activator or gel
red top
Color for sodium citrate tube
blue top
Color for oxalate/fluoride tube
Gray top
Lavender top tube additives
ADDITIVES- contains EDTA, which inhibits coagulation by binding to calcium present in the specimen.-...
Light-blue top tube
ADDITIVE- Contains the anticoagulant Sodium Citrate, which also prevents coagulation by binding...
Green top tube
ADDITIVE- sodium heparin- lithium heparin- ammonium heparinGENERAL- for plasma determinations...
Gray top tube
ADDITIVE- Potassium oxalate (prevents clotting by binding calcium)- Sodium fluoride (preserves...
Red/gray (speckled) top tube
ADDITIVE- Clot activator (glass particles, silica and celite which hastens clot formation -...
Red top
ADDITIVE- none - additive or anticoagulantGENERAL- plain vaccum tube- no inversions- collected...
Pink top
ADDITIVES- contains potassium EDTAGENERAL- used by Blood Banks for molecular and testing of...
Royal Blue
ADDITIVES- Sodium heparin (8 inversions)- Na2 EDTA (8 inversions)- NONECOMMON TESTS- for trace...
Brown test tube
ADDITIVE- Sodium heparin (8 inversions)COMMON TESTS- For lead determinations.- certified to...
Yellow tube
ADDITIVE- Sodium polyanetholesulfonate (8 inversions)GENERAL - For blood culture specimen...

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