Nucleic Acid Amplification

Name the 7 Components of a Typical PCR Reaction.
1. _*_ direct DNA synthesis to the desired region
2. ___ building blocks to extend the primer sequence
3. ___ monovalent cation (salt) for optimal hybridization of primers to template
4. ___ PCR buffer to maintain optimal pH for the enzyme reaction
5. ___ divalent cation required by enzyme
6. ___ enzyme to extend primer sequence
7. ___ DNA to be tested

Name the 7 Components of a Typical PCR Reaction.
1. ___ direct DNA synthesis to the desired region
2. _*_ building blocks to extend the primer sequence
3. ___ monovalent cation (salt) for optimal hybridization of primers to template
4. ___ PCR buffer to maintain optimal pH for the enzyme reaction
5. ___ divalent cation required by enzyme
6. ___ enzyme to extend primer sequence
7. ___ DNA to be tested

Name the 7 Components of a Typical PCR Reaction.
1. ___ direct DNA synthesis to the desired region
2. ___ building blocks to extend the primer sequence
3. _*_ monovalent cation (salt) for optimal hybridization of primers to template
4. ___ PCR buffer to maintain optimal pH for the enzyme reaction
5. ___ divalent cation required by enzyme
6. ___ enzyme to extend primer sequence
7. ___ DNA to be tested

Name the 7 Components of a Typical PCR Reaction.
1. ___ direct DNA synthesis to the desired region
2. ___ building blocks to extend the primer sequence
3. ___ monovalent cation (salt) for optimal hybridization of primers to template
4. _*_ PCR buffer to maintain optimal pH for the enzyme reaction
5. ___ divalent cation required by enzyme
6. ___ enzyme to extend primer sequence
7. ___ DNA to be tested

Name the 7 Components of a Typical PCR Reaction.
1. ___ direct DNA synthesis to the desired region
2. ___ building blocks to extend the primer sequence
3. ___ monovalent cation (salt) for optimal hybridization of primers to template
4. ___ PCR buffer to maintain optimal pH for the enzyme reaction
5. _*_ divalent cation required by enzyme
6. ___ enzyme to extend primer sequence
7. ___ DNA to be tested

Name the 7 Components of a Typical PCR Reaction.
1. ___ direct DNA synthesis to the desired region
2. ___ building blocks to extend the primer sequence
3. ___ monovalent cation (salt) for optimal hybridization of primers to template
4. ___ PCR buffer to maintain optimal pH for the enzyme reaction
5. ___ divalent cation required by enzyme
6. _*_ enzyme to extend primer sequence
7. ___ DNA to be tested

Name the 7 Components of a Typical PCR Reaction.
1. ___ direct DNA synthesis to the desired region
2. ___ building blocks to extend the primer sequence
3. ___ monovalent cation (salt) for optimal hybridization of primers to template
4. ___ PCR buffer to maintain optimal pH for the enzyme reaction
5. ___ divalent cation required by enzyme
6. ___ enzyme to extend primer sequence
7. _*_ DNA to be tested

PCR is an in vitro method to _*_ specific DNA sequences and analyze it at the nucleotide level.

In less than 2 hours, PCR produces copies of amplified DNA sequence called _*_.

The three steps of PCR are _*_, ___ and ___.

The three steps of PCR are ___, _*_ and ___.

The three steps of PCR are ___, ___ and _*_.

The Denaturation step of PCR _*_ the DNA into two strands by heating the sample at ___°C. The length of time for initial denaturation ___ for larger DNA template fragments.

The Denaturation step of PCR ___ the DNA into two strands by heating the sample at _*_°C. The length of time for initial denaturation ___ for larger DNA template fragments.

The Denaturation step of PCR ___ the DNA into two strands by heating the sample at ___°C. The length of time for initial denaturation _*_ for larger DNA template fragments.