Flashcard Set Preview
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| 1 |
The sum of all the chemical processes carried out by living organisms
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metabolism
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reactions that release energy by breaking complex , molecules into simpler ones that...
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catabolism
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taking simple molecules together to make compound molecules
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anabolism
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the loss or removal of electrons
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oxidation
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gain of electrons
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reduction
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a reducing agent is also known as
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an electron donor
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an oxidizing agent is also known as
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electron receptor
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self feeding; use carbon dioxide to synthesize organic molecules
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autotrophy
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other feeding; get their carbon from ready-made organic molecules which they obtain from other...
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heterotrophy
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get their energy from light
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photoheterotrophs (plants) in autotroph
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get their energy by oxidizing simple inorganic molecules such as sulfides and nitrites
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chemoautotrophs (bacteria) in autotrophs
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a series of chemical reactions in which the product of one reaction serves as the substrate for...
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metabolic pathway
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capture energy in a form cells can use
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catabolic pathway
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makes the complex molecules that form the structure of cells, enzymes, and other molecules...
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anabolic pathway
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the energy required to start an enzyme-controlled reaction
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activation energy
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a certain area on the surface of an enzyme where binding takes place
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active site
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the item upon which an enzyme acts
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substrate
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lower activation energy
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enzymes
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do use oxygen (more efficient)
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aerobes
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organisms that do not use oxygen
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anaerobes
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server tissue damage (gangrene)
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Butyric-butylic fermentation
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wine production
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alcohol fermentation
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produces cheese like products
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homolactic acid fermentation
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a way pyruvic acid is metabolized in the absence of oxygen
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fermentation
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metabolic pathway used by most autotrophs and heterotrophsb(sugar spliting)
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glycolysis
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when a non-subtrate molecule binds at a site other than the active site, and causing the active...
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non-competitive inhibitors
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a non-subtrate molecule that competes with the subtrate for the active site on an enzyme
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competitive inhibitor
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usually an inorganic ion, such as magnesium, zinc,, or maganese (minerals)
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cofactor
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a nonprotein organic molecule bound to or loosely associated with an enzyme (vitamins)
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coenzyme
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synthesized in a cell but crosses the cell membrane to act in the periplasmic space or in the...
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exoenzymes
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act within the cell that produces them
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endoenzyme
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refers to an enzymes ability to catalyze only one type of reactions, and act on only one substrate
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specificity
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three names by which the aerobic respiration is known
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originally pyruvic acid cyclekrebs cycletricarboxylic acid cycle citric acid cycle
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the process leading to the transfer of electrons from the substrate to oxygen
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electron transport chain
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how many ATP are formed in the electron transport chain
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30 ATP
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respiration that does not have free oxygen as the final electron
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anaerobic respiration
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the capture of energy from light and the use of this energy to manufracture carbohydrates from...
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photosynthesis
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small group of bacteria that can use energy from light but require organic substances such...
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photoheterotrophy
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bacteria, which are unable to carry out photosynthesis, but can oxidize inorganic sustances...
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chemo autotrophy
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most bacteria move by means of flagella, but some move by gliding or creping or in a corkscrew...
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bacterial movement
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the ability of an organism to emit light; appears to have envolved as a byproduct of aerobic...
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bioluminescence
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many light emmitting organisms have the _____along with other components of the electron transport...
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enzyme luciferase (light bringer)
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original cell, doubled in size before divided
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mother cell
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the name for the two cells after the mother cell divided
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daughter cells
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not cell size but the increase in the number of cells, which occurs by cell division
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microbial growth
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when a cell duplicates its components into 2 cells. The daughter cells become independent when...
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binary fission
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in ____________ the DNA synthesis is continuous and replicated the single bacterial chromosomes...
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prokaryotic cells
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cuboidal groups with four cocci
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tetrads
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groups of eight cocci in a cuboidal packet
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sarcinae
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some Bacilli always form __________ or __________
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chains or filaments
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_______ form chains when grown on artificial media but exist as single or paired cells when...
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streptococci
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when a small new cell develops from the surface of an existing cell and subsequently separates...
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budding
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the four phases of growth
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1. lag phase2. log phase3. the stationary phase4. the decline or death phase
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the 4 phases of growth are known as the??_____
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standard bacterial growth curve
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the organism does not increase significantly in number, they are metabolic active. individuals...
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lag phase
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the organisms divide at their most rapid rate--generation time the population doubles
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log phase
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cell division decreases, new cells are produced at the rate of old cells, the number remains...
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stationary phase
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the number of live cells decreases at a logarithmic rate many cells undergo involution, meaning...
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decline or death phase
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cell divided exponentially with all the descendants of the original cell
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colony
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all phases of growth curve occur simultaneously in a colony. that is growth is_______________
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nonsynchronous
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a method of measurement in which successive 1:10 dilutions are made from an original...
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serial dilution
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made by first adding 0.1ml of a diluted culture to 9ml of melted nutrient agar
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pour plate
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keeps all the cells on the surface of the agar plate, making identification of individual colonies...
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spread plate method
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a number of colonies usually between 30 and 300 on a plate that can be counted with relative...
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countable number
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where actual numbers of a bacteria are counted using specially designed slides (put on a slide)
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direct microscopic count
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a statistical method of measuring growth, used when samples contain too few organisms to give...
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most probable number
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method of estimating bacterial numbers where a known volume of air or water is drawn...
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Filtration
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(cloudy appearance) in a culture indicates the presence or organisms; estimates can be made...
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turbidity
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protons of hydrogen
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pH
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acid loving organisms or environment
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aicidophiles
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like neutral acid environments, human disease causing
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neutrophiles
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alkaline or base loving organisms, way above 7 (ex: vibro chloraea)
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alkaliphiles
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most species of bacteria can grow over a ____ range
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30 degree temp
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cold loving organisms
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psychrophiles
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includes most bacteria groan between 25 and 40, most human pathogens grow best near normal...
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mesophiles
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heat loving organisms
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thermophiles
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means that the organisms must have the specified environmental conditions
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obligate
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means the organism is able to adjust to and tolerate the environmental condition but can also...
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faculative
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cannot grow above 20 degrees
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obligate psychronphiles
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grow best below 20 degrees, but can also grow at higher temps
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facultative psychronphiles
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grown only at temps above 37 degrees
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obligate thermophiles
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can grow above and below 37 degrees
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faculttative thermophiles
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lowest temp at which cells can divide
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minimum growth temp
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highest temp at which cells can divide
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maximum growth temp
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the temp at which cell divide most rapidly, the shortest generation time
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optimum growth temp
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must have free oxygen for aerobic respiration (humans)
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obligate aerobes
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are killed by free oxygen
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obligate anerobes
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grow best in the presence of a small amount of free oxygen
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microaerophiles
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carbon dioxide loving organisms or environment carbon dioxide increasedoxygen decreased
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capnophiles
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carry on aerobic respiration when oxygen is present, but they shift over to anerobic metabolism...
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Facultative aerobes
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survive in the presence of oxygen but dont use it in metabolism
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aerotoleramt anerobes
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the pressure exerted by standing water in proportion to its depth
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hydrostic pressure
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bacteria that live in high pressure, but die in a normal atmosphere
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barophiles
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cell shrinking
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plasmolysis
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plasmoxysis
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cell enlargement
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bacteria that have a special diet or nutritional needs
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fastidious
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Nutrient Sources
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Carbon SourcesNitrogen SourcesSulfur and Phosphorus Trace elements ( copper, zinc, iron,...
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number of nutrients a bacteria must obtain to grow, determined by the number and kind of enzymes
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Nutritional complexity
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usually produced by gram -positive rods
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extracelluar enzymes
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usually produced by gram-negative organisms
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periplasmic enzymes
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the formation of endospores, when nutrients become limiting, a highly resistant endospore forms...
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sporulation
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living part of the endospore
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core
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spherical, thick-walled cells, that resemble endospores
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cysts
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a culture that contains only single species of organism
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pure culture
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bacteria are spread over the surface of an agar plate
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streak plate
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series of dilutions, a sample of bacteria is induced media in a tube of melted agar and the...
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pour plate
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a medium prepared in the lab from the materials of precise and reasonably well-defined composition
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synthetic media
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a media that contains known specific kinds and amounts of chemical substances
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defined synthetic medium
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(chemically non-defined medium)- one that contains reasonably familiar materials...
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complex medium
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a preserved culture that maintains the organisms with the characteristics as originally defined
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reference culture
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a culture in which organisms are maintained in a dormant state
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preserved culture
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minimize the chances that culture will be contaminated by organisms from the environment or...
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aseptic techniques
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a way of maintaining a pure culture, after it has been isolated
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stock culture
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contains special nutrients that allow growth of a particular organism that might not be present...
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enrichment media
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has a constituent that causes an observable change (color or pH) when a particular biochemial...
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differential media
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one that encourages the growth of some organisms but suppress the growth of others
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selective media
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a product of enzyme digestion protein
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peptone
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the liquid part of the blood after clotting factors have been removed
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serum
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made from heated blood, it just looks like chocolate
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chocolate agar
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