Microbiology test 2 chp. 5-7

119 cards

micro chapters 5-7 


 
  
Created Jul 7, 2012
by
bruinaller1910

 

 
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1
The sum of all the chemical processes carried out by living organisms 
 
metabolism 
2
reactions that release energy by breaking complex , molecules into simpler ones that...
 
catabolism 
3
taking simple molecules together to make compound molecules 
 
anabolism
4
the loss or removal of electrons
 
oxidation
5
gain of electrons
 
reduction
6
a reducing agent is also known as 
 
an electron donor 
7
an oxidizing agent is also known as 
 
electron receptor 
8
self feeding; use carbon dioxide to synthesize organic molecules 
 
autotrophy
9
other feeding; get their carbon from ready-made organic molecules which they obtain from other...
 
heterotrophy 
10
get their energy from light 
 
photoheterotrophs  (plants) in autotroph 
11
get their energy by oxidizing simple inorganic molecules such as sulfides and nitrites 
 
chemoautotrophs (bacteria) in autotrophs 
12
a series of chemical reactions in which the product of one reaction serves as the substrate for...
 
metabolic pathway 
13
capture energy in a form cells can use 
 
catabolic pathway 
14
makes the complex molecules that form the structure of cells, enzymes, and other molecules...
 
anabolic pathway
15
the energy required to start an enzyme-controlled reaction 
 
activation energy 
16
a certain area on the surface of an enzyme where binding takes place
 
active site 
17
the item upon which an enzyme acts 
 
substrate 
18
lower activation energy 
 
enzymes 
19
do use oxygen (more efficient) 
 
aerobes
20
organisms that do not use oxygen 
 
anaerobes 
21
server tissue damage (gangrene)
 
Butyric-butylic fermentation 
22
wine production 
 
alcohol fermentation 
23
produces cheese like products 
 
homolactic acid fermentation 
24
a way pyruvic acid is metabolized in the absence of oxygen 
 
fermentation 
25
metabolic pathway used by most autotrophs and heterotrophsb(sugar spliting)
 
glycolysis 
26
when a non-subtrate molecule binds at a site other than the active site, and causing the active...
 
non-competitive inhibitors 
27
a non-subtrate molecule that competes with the subtrate for the active site on an enzyme 
 
competitive inhibitor 
28
usually an inorganic ion, such as magnesium, zinc,, or maganese (minerals)
 
cofactor 
29
a nonprotein organic molecule bound to or loosely associated with an enzyme (vitamins)
 
coenzyme
30
synthesized in a cell but crosses the cell membrane to act in the periplasmic space or in the...
 
exoenzymes
31
act within the cell that produces them 
 
endoenzyme 
32
refers to an enzymes ability to catalyze only one type of reactions, and act on only one substrate 
 
specificity 
33
three names by which the aerobic respiration is known 
 
originally pyruvic acid cyclekrebs cycletricarboxylic acid cycle citric acid cycle 
34
the process leading to the transfer of electrons from the substrate to oxygen 
 
electron transport chain 
35
how many ATP are formed in the electron transport chain 
 
30 ATP 
36
respiration that does not have free oxygen as the final electron 
 
anaerobic respiration 
37
the capture of energy from light and the use of this energy to manufracture carbohydrates from...
 
photosynthesis
38
small group of bacteria that can use energy from light but require organic substances such...
 
photoheterotrophy
39
bacteria, which are unable to carry out photosynthesis, but can oxidize inorganic sustances...
 
chemo autotrophy
40
most bacteria move by means of flagella, but some move by gliding or creping or in a corkscrew...
 
bacterial movement 
41
the ability of an organism to emit light; appears to have envolved as a byproduct of aerobic...
 
bioluminescence
42
many light emmitting organisms have the _____along with other components of the electron transport...
 
enzyme luciferase (light bringer)
43
original cell, doubled in size before divided
 
mother cell
44
the name for the two cells after the mother cell divided 
 
daughter cells 
45
not cell size but the increase in the number of cells, which occurs by cell division
 
microbial growth 
46
when a cell duplicates its components into 2 cells. The daughter cells become independent when...
 
binary fission 
47
in ____________ the DNA synthesis is continuous and replicated the single bacterial chromosomes...
 
prokaryotic cells 
48
cuboidal groups with four cocci
 
tetrads
49
groups of eight cocci in a cuboidal packet
 
sarcinae
50
some Bacilli always form __________ or __________
 
chains or filaments 
51
_______ form chains when grown on artificial media but exist as single or paired cells when...
 
streptococci
52
when a small new cell develops from the surface of an existing cell and subsequently separates...
 
budding 
53
the four phases of growth 
 
1. lag phase2. log phase3. the stationary phase4. the decline or death phase
54
the 4 phases of growth are known as the??_____
 
standard bacterial growth curve
55
the organism does not increase significantly in number, they are metabolic active. individuals...
 
lag phase
56
the organisms divide at their most rapid rate--generation time the population doubles 
 
log phase 
57
cell division decreases, new cells are produced at the rate of old cells, the number remains...
 
stationary phase 
58
the number of live cells decreases at a logarithmic rate many cells undergo involution, meaning...
 
decline or death phase 
59
cell divided exponentially with all the descendants of the original cell 
 
colony
60
all phases of growth curve occur simultaneously in a colony. that is growth is_______________ 
 
nonsynchronous
61
a method of measurement in which successive 1:10 dilutions are made from an original...
 
serial dilution 
62
made by first adding 0.1ml of a diluted culture to 9ml of melted nutrient agar 
 
pour plate 
63
keeps all the cells on the surface of the agar plate, making identification of individual colonies...
 
spread plate method 
64
a number of colonies usually between 30 and 300 on a plate that can be counted with relative...
 
countable number
65
where actual numbers of a bacteria are counted using specially designed slides (put on a slide)
 
direct microscopic count 
66
a statistical method of measuring growth, used when samples contain too few organisms to give...
 
most probable number 
67
method of estimating bacterial numbers where a known volume of air or water is drawn...
 
Filtration
68
(cloudy appearance) in a culture indicates the presence or organisms; estimates can be made...
 
turbidity
69
protons of hydrogen
 
pH
70
acid loving organisms or environment 
 
aicidophiles 
71
like neutral acid environments, human disease causing 
 
neutrophiles
72
alkaline or base loving organisms, way above 7 (ex: vibro chloraea)
 
alkaliphiles
73
most species of bacteria can grow over a ____ range 
 
30 degree temp
74
cold loving organisms 
 
psychrophiles
75
includes most bacteria groan between 25 and 40, most human pathogens grow best near normal...
 
mesophiles
76
heat loving organisms 
 
thermophiles
77
means that the organisms must have the specified environmental conditions 
 
obligate 
78
means the organism is able to adjust to and tolerate the environmental condition but can also...
 
faculative 
79
cannot grow above 20 degrees 
 
obligate psychronphiles
80
grow best below 20 degrees, but can also grow at higher temps 
 
facultative psychronphiles
81
grown only at temps above 37 degrees 
 
obligate thermophiles 
82
can grow above and below 37 degrees 
 
faculttative thermophiles
83
lowest temp at which cells can divide 
 
minimum growth temp
84
highest temp at which cells can divide 
 
maximum growth temp
85
the temp at which cell divide most rapidly, the shortest generation time 
 
optimum growth temp
86
must have free oxygen for aerobic respiration (humans)
 
obligate aerobes 
87
are killed by free oxygen 
 
obligate anerobes 
88
grow best in the presence of a small amount of free oxygen 
 
microaerophiles 
89
carbon dioxide loving organisms or environment carbon dioxide increasedoxygen decreased 
 
capnophiles
90
carry on aerobic respiration when oxygen is present, but they shift over to anerobic metabolism...
 
Facultative aerobes 
91
survive in the presence of oxygen but dont use it in metabolism
 
aerotoleramt anerobes 
92
the pressure exerted by standing water in proportion to its depth 
 
hydrostic pressure 
93
bacteria that live in high pressure, but die in a normal atmosphere 
 
barophiles 
94
cell shrinking 
 
plasmolysis 
95
plasmoxysis 
 
cell enlargement 
96
bacteria that have a special diet or nutritional needs 
 
fastidious 
97
Nutrient Sources 
 
Carbon SourcesNitrogen SourcesSulfur and Phosphorus Trace elements ( copper, zinc, iron,...
98
number of nutrients a bacteria must obtain to grow, determined by the number and kind of enzymes 
 
Nutritional complexity 
99
usually produced by gram -positive rods
 
extracelluar enzymes 
100
usually produced by gram-negative organisms 
 
periplasmic enzymes 
101
the formation of endospores, when nutrients become limiting, a highly resistant endospore forms...
 
sporulation
102
living part of the endospore 
 
core
103
spherical, thick-walled cells, that resemble endospores
 
cysts 
104
a culture that contains only single species of organism 
 
pure culture 
105
bacteria are spread over the surface of an agar plate 
 
streak plate 
106
series of dilutions, a sample of bacteria is induced media in a tube of melted agar and the...
 
pour plate 
107
a medium prepared in the lab from the materials of precise and reasonably well-defined composition 
 
synthetic media
108
a media that contains known specific kinds and amounts of chemical substances 
 
defined synthetic medium 
109
(chemically non-defined medium)- one that contains reasonably familiar materials...
 
complex medium 
110
a preserved culture that maintains the organisms with the characteristics as originally defined 
 
reference culture
111
a culture in which organisms are maintained in a dormant state 
 
preserved culture
112
minimize the chances that culture will be contaminated by organisms from the environment or...
 
aseptic techniques
113
a way of maintaining a pure culture, after it has been isolated 
 
stock culture 
114
contains special nutrients that allow growth of a particular organism that might not be present...
 
enrichment media 
115
has a constituent that causes an observable change (color or pH) when a particular biochemial...
 
differential media 
116
one that encourages the growth of some organisms but suppress the growth of others 
 
selective media 
117
a product of enzyme digestion protein 
 
peptone 
118
the liquid part of the blood after clotting factors have been removed 
 
serum
119
made from heated blood, it just looks like chocolate
 
chocolate agar 

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