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The Indole Test involves which medium?
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A medium containing Tryptophan.
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What is Tryptophan?
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An amino acid abundant in casein.
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What is the reagent used in the indole test?
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Kovac's reagent.
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What is produced in the indole test?
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Indole and NH3
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A red ring in the indole test indicates what?
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Pyruvic acid.
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Give the steps of reduction in the Nitrate Reduction test.
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NO3- is reduced to NO2- (Nitrite) which is reduced to NH3, NO and N2O.
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What 2 reagents are used in the Nitrate Reduction test?
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Sulfonica acid reagent and dimethyl alpha naphthalamine.
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Red indicates a positive test in the nitrate reduction test, If there is no color, what should you add next?
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Zinc. Afterwards, no color is a positive test and red indicates a negative test.
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What is the purpose behind the citrate utilization test?
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To test if the organism can use citrate as the sole organic source of carbon.
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What is the indicator dye for the citrate utilization test? Also, what idicates a positive and negative reaction?
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Bromthymol blue. Green means a negative reaction. Blue means a positive reaction.
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What is the alternative name for the urease test?
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Phenylalanine.
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If FeCl3 is added to the phenylaline test, what indicates a positive reaction.
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Green color.
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What is used in the catalase test? What indicates a positive reaction?
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Hydrogen peroxide. Bubbles.
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What are the products of a catalase test?
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H2O and O2.
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Do anaerobes produce catalase?
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No
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