What Are Various Methods of Sterilization Flashcards

This is the use of specific methods and sterile materials to exclude contaminating microorganisms from an environment.

This is a device employing steam under pressure used for sterilizing materials that are stable to heat and moisture. (15 psi, 121 C for 15 minutes.)

Liquid media used to grow bacteria.

A visible mass of bacterial cells arising from a single cell.

The process of removing or destroying all microorganisms and viruses on or in a product.

Thin wire with a loop on the end used in transferring bacteria to a culture medium.

Thin straight wire used for stabbing agar (to get culture away from oxygen), or for fine manipulations

Population of organisms descending from more than one kind of cell

These are colonies separated from other colonies on a petri dish.

This is a population of organisms descending from a single cell.

The simplest and most commonly used technique for isolating bacteria, a series of successive streak patterns is used to sequentially dilute an inoculum on the surface of an agar plate (non-quantitative dilution.)

Mix culture in a drop of water to give a smooth, even suspension (used for making smears from solid media; i.e. plate or slant)

This technique helps to retain the cells on the slide throughout the staining process.

This is a staining technique that employs one basic dye to impart color to cells.

This is the technique in which the cell is stained but the background remains unstained. Examples: Gram Stain, Spore Stain....