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Cards In This Set
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What is the principle of the catalase test?
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See if org has catalase enzyme which converts H2O2 into H2O + O2 (look for the O2 bubbles)
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What is the principle of the tube coagulase test?
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See if the bug has free coag. if yes, combines with a CRF, then w/ fibrinogen to form a fibrin clot.
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What is the principle of the slide coagulase test?
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See if the bug has bound coag. If yes, the coag can interact directly w/ fibrinogen to form a clot.
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What is the principle of the DNAse Toluidine blue method?
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See if the bug has the enzyme. If yes, it cleaves the DNA. dye is no longer attached and becomes clear.
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What is the principle of the HCL flood plate DNAse test?
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Intact DNA is cloudy. if bug has DNase, it will cleave it and make it soluble (clear)
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What is the principle of the CAMP test?
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See if the bug has CAMP which interacts with staph hemolysin.
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What is the principle of the latex agglutination acid method?
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Add acid, test isolate, neutralizer, Ab coated latex particles. will bind to specific Ag if present. works for all gr except D
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What is the principle of the latex agglutination enzyme extraction method?
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Use pronase B enzyme + test isolate. incubate, add Ab coated latex particles. will bind to Ag if present. works for all grs.
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What is the principle of the PYR test?
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Look to see if bug has PYR enzyme which turns Sutter's reagent (cinnamaldehyde) into B-naphthylamine (pink)
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What is the principle of the hippurate test?
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See if the bug can use hippurate to form glycine which interacts with ninhydrin to form a purple complex.
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What constitutes a positive result in the columbia agar deep test? what bug is positive for this test?
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Orange pigment in the stab line (used the excess starch in the media); gr B strep
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Where do you incubate the Optochin/Taxo P test? What result is considered 'S'? 'R'?
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CO2 incubator; = 14 is susceptible
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What is the principle of the bile solubility test?
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See if the organism can dissolve in bile
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What is the principle of the BEA test? how much bile is in this media?
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See if the bug can withstand 40% bile; see if the bug can hydrolyze esculin to esculetin.
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What is the principle of the NaCl test?
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See if the bug can withstand salt.
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