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Cards In This Set
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What are the components of blood agar?
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5% Sheep Blood
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What is the purpose of using blood agar?
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Grows Gram + and Gram - bacteriaShows hemolytic reactions ( alpha, beta, and gamma)
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What are the components of Chocolate agar?
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2% hgb or IsoVitale X (BBL)Contains X and V Factors
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What are the X and V Factors
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X = HeminV = NAD
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What is the purpose of using Chocolate agar?
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To cultivate Haemophilus sp. (Need X and V)and pathogenic Neisseria sp.
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What are the components of MacConkey agar?
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LactoseCrystal violet and bile salts
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What do crystal violet and bile salts inhibit?
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Gram + bacteria
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What is the purpose of using MacConkey agar?
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To select from Gram - bacteria like EnterobacteriaceaeTo differentiate Lactose and non-Lactose fermenters
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What color do lactose fermenting and non-lactose fermenting colonies appear on MacConkey agar?
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Lactose fermenters = Pink = non-pathogenNon-lactose fermenters = clear = pathogen
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What are the components of MacConkey Sorbitol agar?
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D- sorbitolCrystal violet and bile salts
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What is the purpose of using MacConkey Sorbitol agar?
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To select for E. coli 1057:H7
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What are the components of Campy-Blood agar?
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Vancomycin, trimethoprim, polymixin B, amphotericin B, and cephalothin
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What is the purpose of using Campy-blood agar?
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To select for Campylobacter sp.
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What are the components of Columbia CNA agar?
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Columbia agar with colistin, nalidixic acid and 5% sheep blood
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What does colistin inhibit?
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Gram - bacteria
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