Microbiology Chapter 3

•Inoculation –Addition of microorganism to supporting medium •Incubation –Proper growth temperature and time •Isolation –Creating pure culture from a mixed population •Inspection –Macroscopic and microscopic observations •Identification

•To identify the agent that causes a specific disease requires that microorganisms be isolated and cultivated or cultured

–Inoculum (sample to be cultured)•Environmental specimens (water, air, soil)•Clinical specimens (obtained from patient)•Stored specimens (previously cultured)

–Medium(collection of nutrients allowing growth of inoculum)•Liquid broth •Solid media

–Incubation (placing inoculated media at proper temperature for specific period of time)•Observable growth appears in or on medium after incubation is known as a culture

•A clinical specimen is a sample of human material to be examined or tested for the presence of a pathogen

•Must take care not to contaminate the sample with organisms from the environment or other region’s of the patients body •Must be labeled properly •Must be transported quickly to the lab to avoid death of microbe and minimize growth of normal microbiota

–Technique for isolating the suspected pathogen from normal microbiota in a culture

–refers to a cultures composed of cells arising from a single progenitor (parent or original cell) •Progenitor is termed a CFU (colony forming unit)

Refers to a set of instructions used to prevent further contamination by microbes

•Streak plates •Pour plates

–Used to support the growth of microorganism or as a means of identification

•Liquid •Semi solid •Solid

•Defined media •Complex media •Selective media •Differential media •Anaerobic media •Transport media

–Water-based solution –Growth appears as cloudiness or turbidity