Flashcard Set Preview
| Side A | Side B | ||
| 1 |
Live on above ground surfaces like leaves. (on the plant)
|
Epiphytic bacteria
|
|
| 2 |
Live inside plants (e.g. in vascular tissue)
|
Endophytic bacteria
|
|
| 3 |
Live on the surface of roots
|
Rhizoplane
|
|
| 4 |
Bacteria in the region 'influenced' by roots
|
Rhizosphere
|
|
| 5 |
What is the region 'influenced' by roots?
|
-20% of plant fixed CARBON exuded from root.
-Within mm's of root the bact. pop is higher.
-Diff....
|
|
| 6 |
Fact:
Rhizosphere communities are diff. in composition from bulk soil comm.
|
Fact:
Rhizosphere communities from different plant species are different from each...
|
|
| 7 |
In canola, what is:
a) taken up
b) given
|
a) Gamma-proteobacter is taken in.
b) Holophaga/Acidobacterium is given
|
|
| 8 |
a)Rhizosphere border cells arise from?
b) Are these cells alive or dead?
c) purpose of these...
|
a) Sloughed off plant root tips (root cap/meri-stematic cells)
b) Alive.
c) Possibly a diversionary...
|
|
| 9 |
a) Meaning of epiphyte?
b) Where is an epiphyte?
|
a) epi- "upon" / -phyte "plant"
b) Live on surface (usually on leaves).
|
|
| 10 |
What is DGGE?
|
Denaturing Gradient Gel Electrophoresis.
Uses 16S PCR products. Separates bact. frags. by...
|
|
| 11 |
What is the difference between a regular gel and DGGE?
|
Regular gel separates only by size.
DGGE separates based on size AND sequence.
|
|
| 12 |
What do you use DGGE for?
|
16S fragments from Different Bact.
Different sequences, same size.
|
|
| 13 |
How does DGGE work?
|
Gradient made with denaturant (sep. DNA strands when reaching a 'threshold' (determ. by sequence....
|
|
| 14 |
Diagram DGGE pathway
|
Plant tissue (2 samples)-->PCR amplify 16S-->Seqence AND DGGE
|
|
| 15 |
What can you do with DGGE gel?
|
Excise band--> extract from gel-->insert in plasmid--> express, etc.
|
No comments yet! Be the first to add a comment below!
Please login to post comments.
After login, we will forward you back to this flashcard.