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Cards In This Set
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What does aseptic technique mean?
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Method of handling microbes and materials in a way that minimizes contamination
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What does inoculation mean?
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Process of transferring a microbe from one medium to another
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What is the sample being transferred called?
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Inoculum
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What instrument is a most common one and one that we will use a lot in class?
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Inoculating loop
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What is the inoculating needle used for?
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Used for stabbing or for needing only a small amount of bacteria
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What are the inoculating loop and needle made of?
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Nichrome, a metal alloy that heats up quickly
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What do you use to steralize the loop and needle?
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Bunsen burner
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What color should the flame be on the Bunsen burner?
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Blue
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What is the hottest part of the flame of the Bunsen burner?
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The area just above the inner blue cone
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When removing an inoculum from a liquid culture, how do you resuspend the organisms?
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Roll the tube back and forth between palms, DO NOT SHAKE tube up and down
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To remove the cap of a test tube, which fingers do you use?
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Pinkie and ring finger, be sure to sterilize tube after opening and before recapping
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Which instrument is always used when transferring organisms from a liquid medium?
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Loop
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Why do we tilt a test tube when taking out liquid culture?
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To prevent the culture from pouring out and minimizes the surface area of the tube exposed to contaminants in the air
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How do we remove an inoculum form a slant?
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Keep loop hand still, pull the tube away from the loop, the loop should scrape no more than 1/4 full of the bacteria
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How do we lift the lid from a Petri dish?
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Only about a 45-degree angle, NEVER lift the lid off completely to minmize contamination
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