Hematology Quiz # 2 - Calcs and Manual Analysis

Hematology Quiz # 2

73 cards   |   Total Attempts: 185
  

Cards In This Set

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How do you perform a WBC estimate? Which objective do you use? What is the formula used to calculate it? What are the units?
10x obj. # counted/5 fields x 0.15 = estimated # of WBC. units = 10^9/L
Your WBC count should be within +/- how much % of the MaxM analyzer count?
20%
How do you perform a plt estimate? What obj is used? What is the formula? What are the units
100x oil obj. # of plts counted / 5 fields x 10 = plt estimate. units = 10^9/L
What is the formula to calculate hct? What are the units?
HCT (L/L) = (RBC X MCV) / 1000
What is the formula for calculating MCV? units?
MCV (fL) = Hct/RBC x 1000
What is the formula for calculating MCH? units?
MCH (pg) = Hg/RBC
What is the formula for calculating MCHC? units?
MCHC (g/L) = Hg/Hct
What is the formula for calculating RDW
RDW (%) = SD/MCV x 100
What is RDW-SD? does it depend on MCV?
The width of the RC distn curve at the 20th percentile height; it is independent of MCV.
In what condition would RDW-SD be high? what condition would it be low?
High with iron deficiency anemia. Low with thalassemia.
Is methylene blue a basic or acidic dye? What does it stain?
Basic dye. stains acidic (basophilic) components (like DNA)
Is eosin a basic or acidic dye? What does it stain?
Acidic dye. stains basic (eosinophilic) components (like some granules).
What are the 3 ranges that are established by labs?
Normal, Action, Critical
What are the units for a RBC count?
10^12/L
What is the Electrical Impedance method aka? What kind of info does it give?
Coulter Principle. Info on relative # of cells and cell size only.