Answer These Perls Prussian Blue of Histology Flashcards

Study and answer the Perls Prussian Blue of Histology in a smart manner with these flashcards and revise the concepts related to it. Learn the important terms, definitions, and much more briefly with these Perls Prussian Blue of Histology flashcards quizzes.  

55 cards   |   Total Attempts: 191
  

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What is hemosiderin?
Granules of non-heme iron which have formed water-insoluble complexes with lipids, proteins, and carbohydrates.
How do you make Perl's Incubating mix for the Perl's Prussian Blue stain?
30 mL 2% potassium ferrocyanide 30 mL 0.2 N HCl
How do you prepare the iron counterstain for the Perl's Prussian Blue stain?
100 mL nuclear fast red 50 mL basic fuchsin (pararosaniline)
What is the purpose of the Hgb H stain?
To aid in the diagnosis of alpha thalassemia.
What is the composition of Hgb H?
A tetramer of Beta chains
Why might Hgb F be quantified by alkali denaturation?
To aid in the diagnosis of a thalassemia or hemoglobinopathy. Hgb F is especially high in Hb SS (Sickle cell), unstable hgb, double heterozygous conditions (SC disease, SD, S-thal), and in some cases of aplastic anemia, leukemia and pregnancy.
The Sickle Cell Screening Test: Deoxygenated hemoglobin is insoluble in the presence of a _____ buffer solution and forms a turbid suspension. ____ is used to lyse the red blood cells.
1. phosphate 2. saponin
Explain the heat stability test.
Unstable hemoglobins will frm a flocculent precipitate within one hour of incubation at 50 degrees celsius. Lyse blood with H2O before incubation.
In the visual fluoresence screening test for G6PD deficiency, blood is incubated with ____ and _____. Drops of blood are removed at five minute intervals and spotted on a filter paper and viewed under long wave ultra violet light. What fluoresces, the normal blood are the deficient blood?
Incubate the blood with G6PD and NADP. Normal blood will fluoresce.
Drabkin's reagent is used in the determination of Hgb to convert Hgb to ____ and then to _____.
Methemoglobin, cyanmethemoglobin
What are some conditions that elevate the erythrocyte sedimentation rate?
Lab conditions: - Having a centrifuge on the same bench - Heat - Humidity - Tube at an angle - High anticoagulant to blood ratio Patient conditions: Bacterial infx, multiple myeloma, polycythemia rubra vera
What is the refractive index of coverslip glass and immersion oil?
1.52
Why is NaCl often a diluent in automated cell counters?
It does not corrode glass and tubing, there is a smaller chance of precipitate forming and blocking the tubing, and it allows the particles to become charged so that they can be measured
What does a left shift in an RBC histogram indicate?
Microcytosis
Finding Heinz bodies indicates:
- Chemical poisoning - drug intoxication - G6PD deficiency - Other hemolytic anemia - Unstable hgb (ex Hgb Koln) - Post splenectomy