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Cards In This Set
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What is the equation for doing a cell count on fluids using a hematocytometer?
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# of cells counted x (dilution x 10/# of squares) = cell count
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How do you dilute a very bloody sample?
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1:20, 950 microliters of diluent to 50 microliters of sample
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If you see cells on the hematocytometer, what do you do next?
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Do a differential
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How is a differential performed on a fluid?
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Cytospin 1-4 drops of fluid dispersing on amt of cells in the fluid and count 100 cells. Dilute the sample if necessary.
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What are normal cells seen in a spinal fluid?
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Lymphs, monos, choroid plexus cells, segs
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What are cells that can be found in spinal fluid during a disease state?
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Plasma cell, macrophage, lipophage, increased amount of segs, pyknotic neutrophils, eos, clusters = malignancy, bacteria, malignant cells
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What are normal cells seen in pleural and pericardial fluid?
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Mesothelial cells, segs, lymphs, monos, macrophage
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What are cells that can be seen in pleural and pericardial fluid during a disease state?
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Pyknotic cells, macrophage, LE cells, carcinoma cells, clumps = malignancy
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What are normal cells seen in synovial fluid?
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Synovial lining cells, segs, lymphs
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What are cells that can be seen in synovial fluid during a disease state?
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LE cells, crystals, clumps
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What is the calculation for a manual WBC?
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Number of cells counted x (20 x 10/4) = number of cells
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How is a manual WBC done?
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Dilute the blood by 20, 950 microliters diluent and 50 microliters of blood. count 4 corners, check with bottom and average
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What is the calculation for nucleated rbcs?
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A=BC/C+DA=WBC count corrected for nrbcsB=WBC countC=Constant (100)D=# of nrbcs
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What tests are done to diagnose pernicious anemia?
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Schilling's Test, B12/Folate studies, Intrinsic Factor antibody(also retic ct)
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What tests are done to diagnose Hereditary spherocytosis?
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Osmotic fragility (membrane protein studies may also be done?)
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