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to minimize external contamination of a sample
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1 use an antiseptic-alcohol or betadine(don't use tinture of iodine anymore)2 Use selective...
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Selective media
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inhibits growth of some bacteria and not others
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differential
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differentiates by biochemical reactions of bacteria
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specimen transport
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1 prompt delivery2 sterile container-leak proof is convenient3 transport media- Stuart's, Amies,...
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transport media
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Stuart's-sometimes inhibits growth of gram neg ...
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Alternatives to Prompt delivery/handling
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1 refrigerate-can kill (neisseria and haemophilus)2 special handling-blood at 35-75 degrees,...
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Proper labeling
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NameID numberdatetimespecimen type
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Swabs
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least desirable-dryingtypes-cotton (fa can affect bacteria growth), calcium alginate (can interfere...
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When to use swabs
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skin or mucous membranes-throatcervical, vaginal, urethralanal crypts-gonorrheaerectal swabs...
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Contraindications
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pus or exudate-aspirate-needle and syringesurgical specimen-tissueanaerobes-needle and syringe,...
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