Define Laboratory Techniques in Microbiology Flashcards

Thin layer of cells on a glass microscope slide

Broth culture and slant culture

A technique that permanently adheres bacterial cells to the slide and preserves them in a non-living state without cell distortion of cell morphology.

Heat fixation

Serratia marcescens and Staphylococcus aureus

The solid smear will involve using diluted water

A good smear will be a thin, whitish layer or film. It will aslo have the microbes separated well so that the shape and grouping can be seen.

It will permanently adhere the bacterial cells to the slide and also preserve them in a non-living state with no distortion of cell morphology

Passing the bacterial smear 2-3 times over a medium flame of a Bunsen burner then allowing it to cool before staining

Since all cells have a large percentage of water, the cells are very transparent when seen under a light microscope. Staining makes the contrast more visible

Simple, Differential and Special

1 dye is used and all cells retain the stain. Can determine cell morphology and cell grouping.

2 different dyes. Cells differentiated by end color. Can determine cell morphology, cell grouping, and distinguish one category of bacteria from another category. (gram staining and acid-fast staining.)

Used to highlight and identify bacterial structures. (endospore, flagella, capsule staining.)

Sherical round coccus. Spiral spirillum. Rod bacillus. Curved rod vibrio.