Flashcard Set Preview
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| 1 |
What limits the possible folding of proteins?
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1) Planar nature of the peptide bond
2) Steric hindrance causes some angles of rotation of...
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| 2 |
In the quaternary structure of a protein, what are oligomers hled by?
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Non-covalent linkages like hydrogen bonding or hydrophobic interactions.
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| 3 |
Name three types of oligomers that can exist in the quaternary structure of a protein?
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alpha 2: homodimer
alpha-Beta: heterodimer
alpha2-Beta2: heterotetramer (2 alpha and 2 Beta...
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| 4 |
What happens to the protein structure if you have an amino acid that isn't a helix former/breaker...
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you get a random coil
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| 5 |
Give me a general definition for chromatography
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It's a method for separation of a mixture made up of molecules by passing them through a packed...
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| 6 |
Describe Gel Filtration Chromatography
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Large proteins are excluded from the beads and they travel more rapidly than the small proteins,...
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| 7 |
Describe Ion-Exchange Chromatography
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Certain oppositely charged proteins are attracted to the bead and travel more slowly (or not...
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| 8 |
Affinity Chromatography
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Add a specific binding agent to extract the protein from the matrix.
Then elute by adjusting...
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| 9 |
Hydrophobic Chromatography
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The packed beads are hydrophobic so they denature the protein. The greater the amount of hydrophobic...
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| 10 |
Give me a general definition for Electrophoresis.
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A method for seperating mixtures of molecules based on their movement through an electric field.
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| 11 |
Native PAGE
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Proteins move from the negative to the positive electrode based on how much charge they carry,...
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| 12 |
SDS PAGE
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Move from negative to positive electrode based only on their molecular size. You mix the detergent...
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| 13 |
Isoelectric focusing
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Size doesn't matter; migrate based only on their charge. They move through an "ampholite media"...
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| 14 |
2D Electrophoresis
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A completed Isoelectric focusing gel is used to load an SDS PAGE gel. Combination of charge...
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| 15 |
Give me a general definition for Centrifugation.
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Seperation of mixtures of molecules based on their density.
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| 16 |
Density gradient ultracentrifugation
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Based on density and friction as they "fall" through the tube in a density gradient. The tube...
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| 17 |
Precipitation
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Addition of organic solvents or high salt concentration causes the protein to precipitate at...
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| 18 |
Protein Dialysis
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A method to remove contaminating small molecules from samples of large proteins. A protein...
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| 19 |
What is the key to purification?
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You must consider how the protein you want is different than the contaminating molecules.
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